重组Anti-TMS1/ASC抗体[EPR10402(B)] (ab151700)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10402(B)] to TMS1/ASC
- Suitable for: WB, IP
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TMS1/ASC抗体[EPR10402(B)]
参阅全部 TMS1/ASC 一抗 -
描述
兔单克隆抗体[EPR10402(B)] to TMS1/ASC -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human TMS1/ASC. The exact sequence is proprietary.
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阳性对照
- THP1, MCF7, U937 cell lysates; THP1 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR10402(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab151700于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 22 kDa.
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IP |
1/10 - 1/100.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 22 kDa. |
IP
1/10 - 1/100. |
靶标
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功能
Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. -
组织特异性
Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells. -
序列相似性
Contains 1 CARD domain.
Contains 1 DAPIN domain. -
结构域
Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain. -
翻译后修饰
Phosphorylated. -
细胞定位
Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures. - Information by UniProt
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数据库链接
- Entrez Gene: 29108 Human
- Omim: 606838 Human
- SwissProt: Q9ULZ3 Human
- Unigene: 499094 Human
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别名
- Apoptosis associated speck like protein containing a CARD antibody
- Apoptosis-associated speck-like protein containing a CARD antibody
- ASC antibody
see all
图片
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All lanes : Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700) at 1/5000 dilution (purified)
Lane 1 : THP-1 (Human monocytic leukemia monocyte) whole cell lysate
Lane 2 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 22 kDaBlocking and diluting buffer: 5% NFDM/TBST
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: TMS1/ASC knockout HAP1 cell lysate (20 µg)
Lane 3: U937 cell lysate (20 µg)
Lane 4: THP1 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab151700 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.Unpurified ab151700 was shown to specifically react with TMS1/ASC when TMS1/ASC knockout samples were used. Wild-type and TMS1/ASC knockout samples were subjected to SDS-PAGE. Unpurified ab151700 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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ab151700 (purified) at 1:30 dilution (2ug) immunoprecipitating TMS1/ASC in THP-1 (Human monocytic leukemia monocyte) whole cell lysate.
Lane 1 (input): THP-1 (Human monocytic leukemia monocyte) whole cell lysate 10ug
Lane 2 (+): ab151700 & THP-1 (Human monocytic leukemia monocyte) whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab151700 in THP-1 (Human monocytic leukemia monocyte) whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
All lanes : Anti-TMS1/ASC antibody [EPR10402(B)] (ab151700) at 1/1000 dilution (unpurified)
Lane 1 : THP1 cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : U937 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 22 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab151700 被引用在 8 文献中.
- Lun P et al. HOTTIP downregulation reduces neuronal damage and microglial activation in Parkinson's disease cell and mouse models. Neural Regen Res 17:887-897 (2022). PubMed: 34472490
- Luo XY et al. miR-25-3p ameliorates SAE by targeting the TLR4/NLRP3 axis. Metab Brain Dis 37:1803-1813 (2022). PubMed: 35704145
- Xue S et al. Kui Jie Tong Ameliorates Ulcerative Colitis by Regulating Gut Microbiota and NLRP3/Caspase-1 Classical Pyroptosis Signaling Pathway. Dis Markers 2022:2782112 (2022). PubMed: 35832643
- Gao S et al. LncRNA AK148321 alleviates neuroinflammation in LPS-stimulated BV2 microglial cell through regulating microRNA-1199-5p/HSPA5 axis. Life Sci 266:118863 (2021). PubMed: 33301806
- Dai W et al. lncRNA NEAT1 ameliorates LPS-induced inflammation in MG63 cells by activating autophagy and suppressing the NLRP3 inflammasome. Int J Mol Med 47:607-620 (2021). PubMed: 33416115
- Yue R et al. NLRP3-mediated pyroptosis aggravates pressure overload-induced cardiac hypertrophy, fibrosis, and dysfunction in mice: cardioprotective role of irisin. Cell Death Discov 7:50 (2021). PubMed: 33723236
- Sun H et al. Protective role and molecular mechanism of action of Nesfatin-1 against high glucose-induced inflammation, oxidative stress and apoptosis in retinal epithelial cells. Exp Ther Med 22:833 (2021). PubMed: 34149879
- Sun Z et al. VX765 Attenuates Pyroptosis and HMGB1/TLR4/NF-?B Pathways to Improve Functional Outcomes in TBI Mice. Oxid Med Cell Longev 2020:7879629 (2020). PubMed: 32377306