Key features and details
- Goat polyclonal to TMS1/ASC
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
参阅全部 TMS1/ASC 一抗
特异性This antibody is expected to recognize all three reported isforms (NP_037390; NP_660183; NP_660184).
经测试应用适用于: IHC-P, WBmore details
- HeLa cell lysate Human Liver
This product was previously labelled as TMS1
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab2236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
1 hour primary incubation is recommended for this product.
功能Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.
组织特异性Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells.
序列相似性Contains 1 CARD domain.
Contains 1 DAPIN domain.
结构域Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain.
细胞定位Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures.
- Information by UniProt
- Apoptosis associated speck like protein containing a CARD antibody
- Apoptosis-associated speck-like protein containing a CARD antibody
- ASC antibody