Anti-TMS1/ASC抗体(ab2236)
Key features and details
- Goat polyclonal to TMS1/ASC
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TMS1/ASC抗体
参阅全部 TMS1/ASC 一抗 -
描述
山羊多克隆抗体to TMS1/ASC -
宿主
Goat -
特异性
This antibody is expected to recognize all three reported isforms (NP_037390; NP_660183; NP_660184). -
Tested Applications & Species
Application Species IHC-P HumanWB Human -
免疫原
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阳性对照
- HeLa cell lysate Human Liver
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常规说明
This product was previously labelled as TMS1
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
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IHC-P |
Human
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WB |
Human
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应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
1 hour primary incubation is recommended for this product. |
说明 |
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IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. -
组织特异性
Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells. -
序列相似性
Contains 1 CARD domain.
Contains 1 DAPIN domain. -
结构域
Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain. -
翻译后修饰
Phosphorylated. -
细胞定位
Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures. - Information by UniProt
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数据库链接
- Entrez Gene: 29108 Human
- Omim: 606838 Human
- SwissProt: Q9ULZ3 Human
- Unigene: 499094 Human
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别名
- Apoptosis associated speck like protein containing a CARD antibody
- Apoptosis-associated speck-like protein containing a CARD antibody
- ASC antibody
see all
图片
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Anti-TMS1/ASC antibody (ab2236) at 0.2 µg/ml + HeLa lysate
Developed using the ECL technique.
Predicted band size: 22 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody (ab2236)
Immunohistochemical analysis of human liver tissue staining TMS1/ASC with ab2236 at 4µg/ml. Steamed antigen retrieval with citrate buffer pH6, HRP-staining.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab2236 尚未被引用在任何文献中。