Anti-TMS1/ASC抗体(ab2236)

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Western blot - Anti-TMS1/ASC antibody (ab2236)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody (ab2236)

Key features and details

  • Goat polyclonal to TMS1/ASC
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-TMS1/ASC抗体
    参阅全部 TMS1/ASC 一抗

  • 描述

    山羊多克隆抗体to TMS1/ASC

  • 宿主

    Goat

  • 特异性

    This antibody is expected to recognize all three reported isforms (NP_037390; NP_660183; NP_660184).

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide corresponding to Human TMS1/ASC aa 182-195.
    Sequence:

    C-RESQSYLVEDLERS


    (Peptide available as ab22875, ab8395)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • HeLa cell lysate Human Liver

  • 常规说明

     This product was previously labelled as TMS1

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
IHC-P
Human
WB
Human
应用 Ab评论 说明
IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

1 hour primary incubation is recommended for this product.
说明
IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

1 hour primary incubation is recommended for this product.

靶标

  • 功能

    Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.

  • 组织特异性

    Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and Molt 4 lymphocytic leukemia cells.

  • 序列相似性

    Contains 1 CARD domain.
    Contains 1 DAPIN domain.

  • 结构域

    Interacts with CIAS1/PYPAF1 and PYDC1 via the DAPIN domain.

  • 翻译后修饰

    Phosphorylated.

  • 细胞定位

    Cytoplasm. Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Apoptosis associated speck like protein containing a CARD antibody
      • Apoptosis-associated speck-like protein containing a CARD antibody
      • ASC antibody
      • ASC_HUMAN antibody
      • CARD 5 antibody
      • CARD5 antibody
      • Caspase recruitment domain containing protein 5 antibody
      • Caspase recruitment domain protein 5 antibody
      • Caspase recruitment domain-containing protein 5 antibody
      • hASC antibody
      • MGC10332 antibody
      • PYCARD antibody
      • PYD and CARD domain containing antibody
      • PYD and CARD domain containing protein antibody
      • PYD and CARD domain-containing protein antibody
      • Target of methylation induced silencing 1 antibody
      • Target of methylation-induced silencing 1 antibody
      • TMS 1 antibody
      • TMS antibody
      • TMS1 antibody
      see all

    图片

    • Western blot - Anti-TMS1/ASC antibody (ab2236)
      Western blot - Anti-TMS1/ASC antibody (ab2236)
      Anti-TMS1/ASC antibody (ab2236) at 0.2 µg/ml + HeLa lysate

      Developed using the ECL technique.

      Predicted band size: 22 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody (ab2236)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMS1/ASC antibody (ab2236)

      Immunohistochemical analysis of human liver tissue staining TMS1/ASC with ab2236 at 4µg/ml. Steamed antigen retrieval with citrate buffer pH6, HRP-staining.

       

    文献 (0)

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