Key features and details
- Rabbit polyclonal to TMEM208
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
参阅全部 TMEM208 一抗
经测试应用适用于: WB, ICC/IF, IHC-Pmore details
- Human rectum tissue.
常规说明The antibody solution should be gently mixed before use. ab139459 is a mono-specific antibody. Working dilution samples should be discarded if not used within 12 hours.
存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab130459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
序列相似性Belongs to the TMEM208 family.
- Information by UniProt
- HSPC171 antibody
- TM208_HUMAN antibody
- tmem208 antibody
- Transmembrane protein 208 antibody
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cell.
Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab130459 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab130459, at 1/20 dilution, staining TMEM208 in paraffin-embedded Human rectum tissue by Immunohistochemistry