存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab121565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/250 - 1/500.|
序列相似性Belongs to the TMEM186 family.
- Information by UniProt
- 2810440M13Rik antibody
- 4432406C05Rik antibody
- AI648175 antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane, cytoskeleton (actin filaments) and cytoskeleton (microtubules). Recommended concentration of ab121565 1-4 µg/ml. Cells treated with PFA/Triton X-100.
ab121565 at 1/75 dilution staining TMEM186 in Paraffin-embedded Human bronchus tissue by Immunohistochemistry.
All lanes : Anti-TMEM186 antibody (ab121565) at 1/250 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : TMEM186 transfected HEK293T over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))
Developed using the ECL technique.
ab121565 has not yet been referenced specifically in any publications.