Anti-TMEM16A抗体(ab84915)
Key features and details
- Rabbit polyclonal to TMEM16A
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-TMEM16A抗体
参阅全部 TMEM16A 一抗 -
描述
兔多克隆抗体to TMEM16A -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human TMEM16A aa 450-550 conjugated to keyhole limpet haemocyanin.
(Peptide available asab97423) -
阳性对照
- This antibody gave a positive signal in Human Brain membrane tissue lysate.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab84915于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
Use a concentration of 5 µg/ml.
|
|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa).
|
说明 |
---|
ICC/IF
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa). |
靶标
-
功能
Acts as a calcium-activated chloride channel. Required for normal tracheal development. -
组织特异性
Broadly expressed with higher levels in liver and skeletal muscle. -
序列相似性
Belongs to the anoctamin family. -
结构域
The region spanning the fifth and sixth transmembrane domains probably forms the pore-forming region. -
细胞定位
Cell membrane. Cytoplasm. - Information by UniProt
-
数据库链接
- Entrez Gene: 55107 Human
- Omim: 610108 Human
- SwissProt: Q5XXA6 Human
- Unigene: 503074 Human
-
别名
- ANO 1 antibody
- ANO1 antibody
- ANO1_HUMAN antibody
see all
图片
-
ab84915 staining TMEM16A in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab84915 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
-
Anti-TMEM16A antibody (ab84915) at 1 µg/ml + Human brain normal tissue lysate - membrane extract (ab29456) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 114 kDa
Observed band size: 114 kDa
Additional bands at: 28 kDa, 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab84915 被引用在 1 文献中.
- Luo S et al. Activation of TMEM16A Ca2+-activated Cl- channels by ROCK1/moesin promotes breast cancer metastasis. J Adv Res 33:253-264 (2021). PubMed: 34603794