重组Anti-TMEM161A抗体[EPR14369] (ab180954)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14369] to TMEM161A
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TMEM161A抗体[EPR14369]
参阅全部 TMEM161A 一抗 -
描述
兔单克隆抗体[EPR14369] to TMEM161A -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HT 1080, HepG2 and Human fetal liver lysates; Human lung adenocarcinoma tissue; Human infiltrating duct carcinoma of breast tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR14369 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180954于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/10000 - 1/50000. Detects a band of approximately 54 kDa (predicted molecular weight: 53 kDa).
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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说明 |
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WB
1/10000 - 1/50000. Detects a band of approximately 54 kDa (predicted molecular weight: 53 kDa). |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
May play a role in protection against oxidative stress. Overexpression leads to reduced levels of oxidant-induced DNA damage and apoptosis. -
序列相似性
Belongs to the TMEM161 family. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 54929 Human
- SwissProt: Q9NX61 Human
- Unigene: 631629 Human
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别名
- Adaptive response to oxidative stress protein 29 antibody
- AROS-29 antibody
- T161A_HUMAN antibody
see all
图片
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Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue labeling TMEM161A with ab180954 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling TMEM161A with ab180954 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Anti-TMEM161A antibody [EPR14369] (ab180954) at 1/20000 dilution + Human fetal liver tissue lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted? -
All lanes : Anti-TMEM161A antibody [EPR14369] (ab180954) at 1/100000 dilution
Lane 1 : HT1080 cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab180954 被引用在 1 文献中.
- Chiou SH et al. Global analysis of shared T cell specificities in human non-small cell lung cancer enables HLA inference and antigen discovery. Immunity 54:586-602.e8 (2021). PubMed: 33691136