Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (macaque retina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH 6
Permeabilization
No
Specification
macaque retina
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2.5% serum/PBS
Dilution
1/50
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: donkey anti-mouse AlexaFluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
no distinct staining of retina which is known to be TLR5 positive
Abcam response
We have not tested this antibody with Rhesus sections however as the protein sequence is similar therefore theoretically the antibody should work. We recommend revaluating the sections and optimizing the antigen retrieval step. The antigen retrieval step can be optimized as advised in the following link; https://www.abcam.com/protocols/ihc-antigen-retrieval-protocol.
Contact Abcam scientific support team for further guidelines on troubleshooting.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 14 2017