Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (PFA fixed Brain)
Specification
PFA fixed Brain
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Goat A488 anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Blocking 1hr with NGS 5% in PBS-T
Incubation with Primaries and Secondaries with NGS 5% in PBS-T
Secondaries Incubation time = 3hrs at RT
Abcam response
Abcam has not tested ab13556 in IHC for free-floating sections and thus can't say how it should have performed.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 16 2019