Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Macrophages)
Specification
Macrophages
Fixative
Formaldehyde
Permeabilization
Yes - .1% TritonX-100 in 3% BSA
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA
Secondary antibody
Name
Non-Abcam antibody was used: sc-2783
Host species: Donkey
Clonality: Polyclonal
Conjugation: Texas Red ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Texas Red ®
Dilution
1/100
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Varkha Agrawal
Verified customer
提交于 Oct 18 2012