Anti-TLR3抗体[40C1285] (ab13915)


  • 产品名称
    参阅全部 TLR3 一抗
  • 描述
    小鼠单克隆抗体[40C1285] to TLR3
  • 宿主
  • 特异性
    Negative results in WB have been obtained for mouse interstine lysates (human intestine is a positive control). The protein is heavily glycosylated and the observed band size should be higher than the predicted 103kDa. The protein is found at different molecular weight in different tissues so optimization may be necessary.
  • 经测试应用
    适用于: IHC-P, WB, Flow Cyt, IPmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide:


    conjugated to KLH, corresponding to amino acids 55-70 of Human TLR3

  • 阳性对照
    • Human intestine tissue lysate, mouse spleen



Our Abpromise guarantee covers the use of ab13915 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 - 10 µg/ml. Permeabilization of samples might be necessary and overnight incubation with the ab13915 is recommended.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 108 kDa). TLR3 is a single-pass type I membrane protein. We recomend not to boil the samples, instead heat lysates at 60-70C for 10 minutes.
Flow Cyt Use 2-5µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


IP Use 2µg for 106 cells.


  • 功能
    Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific of microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
  • 组织特异性
    Expressed at high level in placenta and pancreas. Also detected in CD11c+ immature dendritic cells. Only expressed in dendritic cells and not in other leukocytes, including monocyte precursors. TLR3 is the TLR that is expressed most strongly in the brain, especially in astrocytes, glia, and neurons.
  • 疾病相关
    Defects in TLR3 are associated with herpes simplex encephalitis type 2 (HSE2) [MIM:613002]. HSE is a rare complication of human herpesvirus 1 (HHV-1) infection, occurring in only a small minority of HHV-1 infected individuals. HSE is characterized by hemorrhagic necrosis of parts of the temporal and frontal lobes. Onset is over several days and involves fever, headache, seizures, stupor, and often coma, frequently with a fatal outcome. Note=TLR3 mutations predispose otherwise healthy individuals to isolated herpes simplex encephalitis through a mechanism that involves impaired IFNs production and reduced immune defense against viral infection in the central nervous system.
  • 序列相似性
    Belongs to the Toll-like receptor family.
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.
    Contains 1 TIR domain.
  • 结构域
    ds-RNA binding is mediated by LRR 1 to 3, and LRR 17 to 18.
  • 翻译后修饰
    Heavily N-glycosylated, except on that part of the surface of the ectodomain that is involved in ligand binding.
  • 细胞定位
    Endoplasmic reticulum membrane. Endosome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD283 antibody
    • CD283 antigen antibody
    • IIAE2 antibody
    • TLR 3 antibody
    • Tlr3 antibody
    • TLR3_HUMAN antibody
    • Toll Like Receptor 3 antibody
    • Toll-like receptor 3 antibody
    see all


  • All lanes : Anti-TLR3 antibody [40C1285] (ab13915) at 3 µg/ml

    Lane 1 : Untransfected HEK293 cell lysate
    Lane 2 : HEK293 cell lysate transfected with human TLR3 cDNA whole cell lysate
    Lane 3 : Human intestine tissue lysate
    Lane 4 : Human placenta tissue lysate
    Lane 5 : Human heart tissue lysate
    Lane 6 : Human ovary tissue lysate

    All lanes : Goat anti-mouse HRP conjugate

    Predicted band size: 108 kDa

  • Immunohistochemical analysis of formalin fixed paraffin embedded tissue section of mouse intestine using ab13915 at 1/500 dilution with HRP-DAB detection and hematoxylin counterstaining. The image shows a punctate staining of the ER and endosomes in a subset of cells in Peyer's patches (organized lymphoid nodules) in the tested section.

  • Flow cytometry analysis of human B cells using ab13915 at 1µg/106 cells followed by FITC conjugated secondary antibody (Red). Green shows isotype control.  

  • Western blot analysis of TLR3 in lysates from untransfected 293 cells (lane A), 293 cells transfected with human TLR3 cDNA (lane B), and 20 µg/lane human intestine tissue lysate (lane C).

    Western blot analysis of TLR3 in lysates from untransfected 293 cells (lane A), 293 cells transfected with human TLR3 cDNA (lane B), and 20 µg/lane human intestine tissue lysate (lane C).
  • Immunohistochemical analysis of formalin fixed paraffin embedded tissue section of mouse colon using ab13915 at 1/500 dilution with HRP-DAB detection and hematoxylin counterstaining. Intense signal was found in subset of cells at the bases of the crypts.

  • ab13915 at 2µg / 1x106 cells staining intracellular TLR3 in human monocytes by Flow Cytometery. A PE-conjugated goat polyclonal to mouse IgG1 was used as secondary. The shaded histogram repersents monocytes without antibody; green repersents isotype control and red histogram shows staining with ab13915. 

  • ab13915 at 5ug/ml staining TLR3 in mouse spleen tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). The left image repersents staining with isotype control and right one repersents staining with ab13915.

  • ab13915 staining TLR3 in SW480, SW620 (colon cancer) and FaDu, Detroit 562 obtained from human cells by Flow Cytometry. Samples were prepared by trypsinizining and washing in PBS and centrifugation in a buffer containing 2% FBS, 0.1% Na-azide and PBS. Cells were fixed in paraformaldehyde and permeabilized in 0.2% Tween. Gating and analysis was performed by WinMDI 2.9 software. The primary antibody was diluted to 1 µg/cells (PBS, 2% FBS and 0.1% Na-azide) and incubated with sample for 30 minutes at 4°C. An FITC conjugated rabbit polyclonal to mouse IgG, diluted to 1/40, was used as secondary. Image represents FACS analysis of TLR3 protein expression in SW480 (A), SW620 (B), FaDu (C) and Detroit 562 (D) cell lines. Cell lines were permeabilized and immunostained with TLR3-specific antibody (thick black l

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This product has been referenced in:
  • Ni YH  et al. Distinct expression patterns of Toll-like receptor 7 in tumour cells and fibroblast-like cells in oral squamous cell carcinoma. Histopathology 67:730-9 (2015). IHC . Read more (PubMed: 25828894) »
  • Allman WR  et al. TACI deficiency leads to alternatively activated macrophage phenotype and susceptibility to Leishmania infection. Proc Natl Acad Sci U S A 112:E4094-103 (2015). Mouse . Read more (PubMed: 26170307) »

See all 13 Publications for this product


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Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Mouse Tissue lysate - whole (liver)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

提交于 Dec 01 2014

Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 25°C
Mouse Cell (Macrophages)
Yes - Triton X 0.1% 5 min

Abcam user community

Verified customer

提交于 Jul 23 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Flow Cytometry
Human Cell (SW480, SW620 (colon cancer) and Fadu, Detroit 562)
SW480, SW620 (colon cancer) and Fadu, Detroit 562
Cell harvesting/tissue preparation method: Cells were trypsinized and washed in PBS.
Sample buffer: 2% FBS/0.1%Na-azide/PBS
Yes - 0.2% tween
Gating Strategy
There was only one population visible after the staining. Gating and analysis was performed by WinMDI 2.9 software.

Mrs. Tanja Matijevic

Verified customer

提交于 Jul 14 2009