概述

  • 产品名称
    Anti-TLR2抗体[TL2.1]
    参阅全部 TLR2 一抗
  • 描述
    小鼠单克隆抗体[TL2.1] to TLR2
  • 宿主
    Mouse
  • 经测试应用
    适用于: ICC/IF, Flow Cyt, IP, Neutralising, WB, Functional Studiesmore details
  • 种属反应性
    与反应: Dog, Human, Cynomolgus monkey, Rhesus monkey, Common marmoset
  • 免疫原

    Recombinant human TLR2 produced by the CHO/CD14 reporter cell line.

  • 阳性对照
    • WB: THP1 + LPS. IHC-P: Human TLR2. ICC: Human U937 cells. Flow: Human peripheral blood mononuclear cells.
  • 常规说明

    Ab9100 is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human pheriperal blood mononuclear cells.

性能

应用

Our Abpromise guarantee covers the use of ab9100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 - 10 µg/ml.

Fix with PBS/2% formalin for 15 min at 4°C; permeabilize with acetone, 10 min, -20°C (see Flo et al J Leukoc Biol which used a conjugated form of the antibody).

Flow Cyt Use 2-3µg for 105 cells.

Untreated and 0.4 % formaldehyde fixed cells can be used; do not permeabilize the cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.
Neutralising Use at an assay dependent concentration. See Lien et al.
WB 1/50.

Abcam recommends using non-reducing conditions.

Functional Studies Use at an assay dependent concentration.

靶标

  • 功能
    Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage-activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6.
  • 组织特异性
    Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues.
  • 序列相似性
    Belongs to the Toll-like receptor family.
    Contains 14 LRR (leucine-rich) repeats.
    Contains 1 TIR domain.
  • 翻译后修饰
    Glycosylation of Asn-442 is critical for secretion of the N-terminal ectodomain of TLR2.
  • 细胞定位
    Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD282 antibody
    • CD282 antigen antibody
    • TIL 4 antibody
    • TIL4 antibody
    • TLR 2 antibody
    • TLR2 antibody
    • TLR2_HUMAN antibody
    • Toll like receptor 2 antibody
    • Toll like receptor 2 precursor antibody
    • Toll-like receptor 2 antibody
    • Toll/interleukin 1 receptor like 4 antibody
    • Toll/interleukin 1 receptor like protein 4 antibody
    • Toll/interleukin receptor like protein 4 antibody
    • Toll/interleukin-1 receptor-like protein 4 antibody
    see all

图片

  • TLR2 staining on human peripheral blood mononuclear cells by using ab9100. Green: CD3+-cells; Red: CD14+ cells
  • All lanes : Anti-TLR2 antibody [TL2.1] (ab9100) at 1 µg/ml

    Lane 1 : THP1
    Lane 2 : THP1 + LPS

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution

    Performed under reducing conditions.


    This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was ran at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked with 2% BSA for 60 minutes before being incubated with ab9100 (anti-TLR2 antibody; 1 ug/mL) for 18 hours at 4°C. Antibody binding was detected using ab97040 (HRP-labelled goat anti-mouse IgG)  at  1:50,000 dilutions for 1 hour at room temperature.Visualisation was conducted using ECL development solution ab133406.

    This gel was run under reducing conditions resulting in weak signal. We recommend customers to use non-reducing conditions.

  • ab9100 staining TLR2 in human U937 cells by Immunocytochemistry. Cells were fixed with paraformaldehyde and blocking with 2% BSA was done for 30 minutes at 270C. Samples were incubated with primary antibody (1/50) for 1 hour at 27°C. An Alexa Fluor®568-conjugated goat polyclonal to mouse IgG (H&L) was used at dilution at 1/50 as secondary antibody.

    See Abreview

文献

This product has been referenced in:
  • Galoian K  et al. Toll like receptors TLR1/2, TLR6 and MUC5B as binding interaction partners with cytostatic proline rich polypeptide 1 in human chondrosarcoma. Int J Oncol 52:139-154 (2018). Read more (PubMed: 29138803) »
  • Moretti IF  et al. Plasmatic membrane toll-like receptor expressions in human astrocytomas. PLoS One 13:e0199211 (2018). Read more (PubMed: 29912993) »
See all 21 Publications for this product

客户评价及客户问答

1-10 of 30 Abreviews or Q&A

Answer

Thank you for your inquiry.

I can confirm that the storage temperature of our products can be found on the individual datasheets.

Short term storage is usually two weeks. If the products is not used within two weeks of purchase, we in general recommend to aliquot it and to store as stated for the long term storage. As a general rule, freeze/thaw cycle are to be avoided.

Please find below the long term storage conditions for the products you are interested in:

ab9100 : Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

ab3358 : Store at +4°C.

ab3359 : Store at +4°C.

ab30667 : Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

ab86128 : Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Since the vials are small and we want the text to be readable, we strongly suggest to check the datasheets for the storage information. This will have the positive side effect that also all the other valuable information on the datasheet will be seen.

I hope this information is helpful and wish you a sunny day.

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Question
Answer

I am very pleased to hear you would like to accept our offer and test ab9100, ab17044, and ab117645 in rabbit. Each code will give you 1 free primary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forrabbit and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for getting back to me and for getting in touch with BioConnect. I will let you know if I don't hear anything from them shortly, and we will figure out the vial of ab9669. I'll also send you a confirmation email after I work everything out with them.

Thank you for your understanding, and let me know if you have any questions in the meantime.

Read More
Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (macrophage)
Specification
macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Dec 12 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Pig Cell (freshly isolated PBMC)
Permeabilization
No
Gating Strategy
living singlets
Specification
freshly isolated PBMC

Korneel Grauwet

Verified customer

提交于 Aug 01 2014

Answer

Thank you for your enquiry regarding.

I can confirm that ab9100 has been tested for functional and neutralizing assay whilst the other antibody has not (ab8376).

I regret to inform you that we have not conducted any studies to determine whether the antibody (ab9100) retains its functional activity during an experiment for over 4 days. This would depend on the assay conditions and other reagents used in the experimental set up. What we know is that the antibody is stable for a number of days at 37°C.

Furthermore, our own experience for in vitro testing is that the antibody works well in an overnight experiment.

If you need any further assistance, please do not hesitate to contact me.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Rabbit Tissue lysate - whole (Brain)
Loading amount
25 µg
Specification
Brain
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-20% gradient)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

提交于 Apr 13 2012

Answer

Thank you for sending that email.

Unfortunately, none of the protocol information or data came through in the email. Could you please resend your protocol information and data, so that I can try and help resolve the issues you are having.

Looking forward to you reply.

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Answer

Thank you for your reply, and I apologize for this inconvenience.

I certainly understand that ab9858 is not as useful to you as ab73680 would be. I can issue a credit to you.

The vial of ab8020 should be arriving shortly, please keep me updated about the results with this replacement, and let me know if there is anything else that you need!

Read More

Answer

Thank you again for your patience.

I apologize, but ab73680 is out of stock, and it may not be put back into stock for several months as recent lots have not passed quality control testing. Would you be interested in trying another vial of ab9669, or ab9858-

https://www.abcam.com/MCP1-antibody-ab9858.html

Please let me know and I will arrange for this to be sent to you promptly. Have a great day!

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1-10 of 30 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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