Anti-Tissue kallikrein抗体(ab28289)
Key features and details
- Rabbit polyclonal to Tissue kallikrein
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Tissue kallikrein抗体
参阅全部 Tissue kallikrein 一抗 -
描述
兔多克隆抗体to组织kallikrein -
宿主
Rabbit -
特异性
ab28289 recognises the Kallikrein loop of Tissue kallikrein. It does not react with the other human Kallikreins. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Tissue kallikrein. Corresponding to the the Kallikrein loop area of Tissue kallikrein.
(Peptide available asab41419) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28289于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 28 kDa.
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 28 kDa. |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. -
组织特异性
Isoform 2 is expressed in pancreas, salivary glands, kidney, colon, prostate gland, testis, spleen and the colon adenocarcinoma cell line T84. -
序列相似性
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
翻译后修饰
The O-linked polysaccharides on Ser-93, Ser-104 and Ser-167 are probably the mucin type linked to GalNAc. In PubMed:3163150, GalNAc was detected with the corresponding peptides but not located. - Information by UniProt
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数据库链接
- Entrez Gene: 3816 Human
- Omim: 147910 Human
- SwissProt: P06870 Human
- Unigene: 123107 Human
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别名
- Glandular kallikrein 1 antibody
- hK 1 antibody
- hK1 antibody
see all
图片
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All lanes : Anti-Tissue kallikrein antibody (ab28289) at 1/1000 dilution
Lane 3 : Kallikrein-1 (rhK-1) at 0.05 µg
Lane 4 : Kallikrein-1 (rhK-1) at 0.01 µg
Lane 5 : Kallikrein-1 (rhK-1) at 0.001 µg
Lane 7 : Kallikrein-3 (rhK-3) at 0.05 µg
Lane 8 : Kallikrein-3 (rhK-3) at 0.01 µg
Lane 9 : Kallikrein-3 (rhK-3) at 0.001 µg
Lane 11 : Kallikrein-4 (rhK-4) at 0.05 µg
Lane 12 : Kallikrein-4 (rhK-4) at 0.01 µg
Lane 13 : Kallikrein-4 (rhK-4) at 0.001 µg
Predicted band size: 28 kDa -
IHC image of ab28289 staining in human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab28289, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab28289 被引用在 4 文献中.
- Huang S et al. Co-expression of tissue kallikrein 1 and tissue inhibitor of matrix metalloproteinase 1 improves myocardial ischemia-reperfusion injury by promoting angiogenesis and inhibiting oxidative stress. Mol Med Rep 23:N/A (2021). PubMed: 33355364
- Zhang M et al. Human Tissue Kallikrein 1 Is Downregulated in Elderly Human Prostates and Possesses Potential In Vitro Antioxidative and Antifibrotic Effects in Rodent Prostates. Oxid Med Cell Longev 2021:8877540 (2021). PubMed: 34007408
- Tang SC et al. Bradykinin and high glucose promote renal tubular inflammation. Nephrol Dial Transplant 25:698-710 (2010). ICC/IF ; Human . PubMed: 19923143
- Cassim B et al. Kallikreins, kininogens and kinin receptors on circulating and synovial fluid neutrophils: role in kinin generation in rheumatoid arthritis. Rheumatology (Oxford) 48:490-6 (2009). ICC/IF, IHC ; Human . PubMed: 19254919