概述

  • 产品名称
    Anti-Tissue Factor抗体
    参阅全部 Tissue Factor 一抗
  • 描述
    兔多克隆抗体to组织Factor
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Recombinant fragment, corresponding to amino acids 33 - 295 of Human Tissue Factor (BC011029).

  • 阳性对照
    • WB: Human fetal kidney, brain, skin and lung lysates IHC-P: Human fetal kidney tissue

性能

应用

Our Abpromise guarantee covers the use of ab104513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/100 - 1/500.

靶标

图片

  • All lanes : Anti-Tissue Factor antibody (ab104513) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : Human fetal skin lysate
    Lane 4 : Human fetal lung lysate

    Predicted band size: 33 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

  • ab104513, at a 1/100 dilution, staining Tissue Factor in formalin-fixed, paraffin-embedded Human fetal kidney tissue, showing membrane staining.

文献

This product has been referenced in:
  • Li Y  et al. Mechanism of the protective effects of the combined treatment with rhynchophylla total alkaloids and sinapine thiocyanate against a prothrombotic state caused by vascular endothelial cell inflammatory damage. Exp Ther Med 13:3081-3088 (2017). Read more (PubMed: 28587383) »
  • Abu El-Asrar AM  et al. Coexpression of heparanase activity, cathepsin L, tissue factor, tissue factor pathway inhibitor, and MMP-9 in proliferative diabetic retinopathy. Mol Vis 22:424-35 (2016). WB . Read more (PubMed: 27168718) »
See all 8 Publications for this product

客户评价及客户问答

1-6 of 6 Abreviews or Q&A

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Mouse Cell lysate - other (aortic vascular smooth muscle cells)
Specification
aortic vascular smooth muscle cells
Treatment
Indoxy sulfate 0.25-100 ug/ml
Blocking step
Milk as blocking agent for 45 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C

Dr. Moshe Shashar

Verified customer

提交于 Jul 18 2014

Question
Answer

Thank you for contacting us.


I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid.
(2) Held on the account for use against a future order.
(3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

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Question
Answer

Thanks for the additional information.

I have initiated the request for a replacement of one vial of ab104513. Please contact me again if you have any further questions.

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Question

Inquiry: Order Details: Antibody code: ab104513 Batch number: GR28719-4 General Information: Antibody storage conditions (temperature/reconstitution etc) – sample was aliquoted into smaller volumes and stored in freezer (-20°C) Description of the problem (high background, low signal, non-specific staining etc.) – Non-specific (inappropriate) staining. We noticed color spots on some irrelevant areas of the slide. It seemed like we added some coloured not soluble Ab sample. Sample (Species/Tissue/Cell Type/Cell Line etc.). Human lung cancer paraffin embedded sections Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.). Formalin Antigen retrieval (Enzymatic method, Heat mediated technique etc.). Water bath 60 min 95°C in Zytomed Systems OmniPrep Solution Permeabilization step – we do not need it and didn’t perform Blocking conditions (Buffer/time period, Blocking agent etc.) Protein Block solution from the REVEAL Rabbit Specific AP Conjugate kit (Spring) (0.5% BSA, 0.5% casein) Primary Antibody (Diluent/Dilution/Incubation time, Wash step): aTF diluted 1:100 and 1:40 in the antibody diluent from the kit (PBS-0.1% BSA). Incubation at 4°C, o/n. Washing in 1XPBS/0.1% Tween-20 Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step). REVEAL Rabbit Specific AP Conjugate kit (Spring) – ready to use – 30 min at RT Detection method Naphhtol Phosphate + Fast Red Chromogen (kit) Positive and negative controls used (please specify). Positive/negative controls: rabbit anti human CD31, VEGF, VEGFR, PDGFR, Tie2, normal rabbit IgG Optimization attempts (problem solving): How many times have you tried the IHC? Twice in two different dilutions Have you run a "No Primary" control? Yes – the control of the AP kit, without 1st Ab introduced Do you obtain the same results every time? Yes (twice) What steps have you altered? We didn’t. We are working with patient biopsies where the amount of tissue available is limited and not enough for different staining protocol alterations. In the manufacturer product data sheet not even the incubation terms are recommended Additional Notes: Document Attachment: images of the results may be very helpful. If you wish to add it to the complaint form, please attach them to the reply e-mail.

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Answer

Thank you for submitting your completed questionnaire.

It does appear that this antibody is not working as expected. If you have received this antibody within the past 6 months or so I would be happy to offer a replacement.

In your reply, please include the PO number or Abcam order reference number so I can process your request.

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Application
Flow Cytometry
Sample
Rat Cell (Plasma Microparticles)
Specification
Plasma Microparticles
Preparation
Cell harvesting/tissue preparation method: Centrifuge whole blood 1,500xg 15 min, then centrifuge the supernatant again at 13,000xg 2 min, and retain supernatant
Sample buffer: Annexin Binding Buffer
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
MP samples without label, and sample with secondary antibody only

Abcam user community

Verified customer

提交于 Feb 23 2012

Question
Answer

I am very pleased to hear you would like to accept our offer and test ab104513 in flow cytometry using rat samples. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forflow cytometry using rat samples and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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