Key features and details
- Mouse monoclonal [3A4] to TIMP2
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Rabbit, Guinea pig, Cow, Human
- Isotype: IgG2a
参阅全部 TIMP2 一抗
描述小鼠单克隆抗体[3A4] to TIMP2
经测试应用适用于: IHC-P, IHC-Frmore details
种属反应性与反应: Mouse, Rat, Rabbit, Guinea pig, Cow, Human
Synthetic peptide corresponding to Human TIMP2 (N terminal).
- IHC-P: Normal human placenta tissue.
This antibody clone is manufactured by Abcam. If you require it in a particular buffer formulation or a particular conjugate for your experiments, please contact email@example.com.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.02% Sodium azide
Concentration information loading...
纯度Protein G purified
Our Abpromise guarantee covers the use of ab1828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|IHC-Fr||1/100. PubMed: 24343133|
功能Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
序列相似性Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
翻译后修饰The activity of TIMP2 is dependent on the presence of disulfide bonds.
- Information by UniProt
- CSC 21K antibody
- CSC-21K antibody
- CSC21K antibody
IHC image of TIMP2 staining in a section of formalin-fixed paraffin-embedded normal human placenta* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab1828, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
ab1828 被引用在 41 文献中.
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- Xie X et al. Fe3O4-solamargine induces apoptosis and inhibits metastasis of pancreatic cancer cells. Int J Oncol 54:905-915 (2019). PubMed: 30483763
- Burduk PK et al. Expression of Matrix Metalloproteinase-2/9 and Tissue Inhibitor of Metalloproteinase-1/2 as Predictive Factors in Oropharyngeal Squamous Cell Carcinoma. Iran J Otorhinolaryngol 31:153-161 (2019). PubMed: 31223594