Anti-TIMP2抗体[3A4] (ab1828)
Key features and details
- Mouse monoclonal [3A4] to TIMP2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
概述
-
产品名称
Anti-TIMP2抗体[3A4]
参阅全部 TIMP2 一抗 -
描述
小鼠单克隆抗体[3A4] to TIMP2 -
宿主
Mouse -
Tested Applications & Species
Application Species IHC-P Human -
免疫原
Synthetic peptide corresponding to Human TIMP2 (N terminal).
-
阳性对照
- IHC-P: Normal human placenta tissue.
-
常规说明
This antibody clone is manufactured by Abcam. If you require it in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
3A4 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1828 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
---|---|
IHC-P |
Human
|
All applications |
Mouse
Rat
Rabbit
Guinea pig
Cow
|
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (1) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
说明 |
---|
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
-
功能
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. -
序列相似性
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
翻译后修饰
The activity of TIMP2 is dependent on the presence of disulfide bonds. -
细胞定位
Secreted. - Information by UniProt
-
数据库链接
- Entrez Gene: 282093 Cow
- Entrez Gene: 100135629 Guinea pig
- Entrez Gene: 7077 Human
- Entrez Gene: 21858 Mouse
- Entrez Gene: 100008689 Rabbit
- Entrez Gene: 29543 Rat
- Omim: 188825 Human
- SwissProt: P16368 Cow
see all -
别名
- CSC 21K antibody
- CSC-21K antibody
- CSC21K antibody
see all
图片
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP2 antibody [3A4] (ab1828)
IHC image of TIMP2 staining in a section of formalin-fixed paraffin-embedded normal human placenta* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab1828, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
数据表及文件
-
SDS download
-
Datasheet download
文献 (45)
ab1828 被引用在 45 文献中.
- Li S et al. MicroRNA-4500 Inhibits Migration, Invasion, and Angiogenesis of Breast Cancer Cells via RRM2-Dependent MAPK Signaling Pathway. Mol Ther Nucleic Acids 21:278-289 (2020). PubMed: 32615527
- Jiang X et al. Inhibition of Triple-Negative Breast Cancer Tumor Growth by Electroacupuncture with Encircled Needling and Its Mechanisms in a Mice Xenograft Model. Int J Med Sci 16:1642-1651 (2019). PubMed: 31839752
- Li YJ et al. Long noncoding RNA ZNF667-AS1 reduces tumor invasion and metastasis in cervical cancer by counteracting microRNA-93-3p-dependent PEG3 downregulation. Mol Oncol 13:2375-2392 (2019). PubMed: 31420931
- Tian G et al. Ethanol extract of Ligustrum lucidum Ait. leaves suppressed hepatocellular carcinoma in vitro and in vivo. Cancer Cell Int 19:246 (2019). PubMed: 31572063
- Cui LH et al. Saikosaponin d ameliorates pancreatic fibrosis by inhibiting autophagy of pancreatic stellate cells via PI3K/Akt/mTOR pathway. Chem Biol Interact 300:18-26 (2019). PubMed: 30611790