Name: Anti-TIMP1 antibody [EPR18352]
Brand: RabMAb
SKU: ab211926
Rating: 5 (9 reviews)

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Western blot - Anti-TIMP1 antibody [EPR18352] (ab211926)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18352] to TIMP1
Suitable for: IHC-P, WB, ICC/IF
Knockout validated
Reacts with: Human

产品名称

Anti-TIMP1抗体[EPR18352]
参阅全部 TIMP1 一抗
描述

兔单克隆抗体[EPR18352] to TIMP1
宿主

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

免疫原

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: Human prostate cancer lysate; HeLa, SK-OV-3 and HT-29 whole cell lysates. IHC-P: Human colon, pancreas, lung cancer, medullary thyroid carcinoma and prostate cancer tissues. ICC/IF: SK-OV-3 and HT-29 cells.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR18352
同种型

IgG
研究领域

Alternative Versions
- Anti-TIMP1 antibody [EPR18352] - BSA and Azide free (ab219471)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant human TIMP1 protein (ab82104)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab211926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用	Species
ICC/IF	Human
IHC-P	Human
WB	Human

应用	Ab评论	说明
IHC-P	(3)	1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB	(1)	1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
ICC/IF	(2)	1/500.

说明
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).
ICC/IF 1/500.

功能

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
序列相似性

Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
翻译后修饰

The activity of TIMP1 is dependent on the presence of disulfide bonds.
细胞定位

Secreted.
Target information above from: UniProt accession P01033 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 7076 Human
- Omim: 305370 Human
- SwissProt: P01033 Human
- Unigene: 522632 Human
别名
- Clgi antibody
- Collagenase inhibitor antibody
- Collagenase inhibitor, Human antibody
- EPA antibody
- EPO antibody
- Erythroid Potentiating Activity antibody
- Erythroid-potentiating activity antibody
- Fibroblast collagenase inhibitor antibody
- FLJ90373 antibody
- HCI antibody
- Human Collagenase Inhibitor antibody
- Metalloproteinase inhibitor 1 antibody
- Metalloproteinase inhibitor 1 precursor antibody
- OTTHUMP00000023214 antibody
- TIMP 1 antibody
- TIMP antibody
- TIMP metallopeptidase inhibitor 1 antibody
- TIMP-1 antibody
- Timp1 antibody
- TIMP1 protein antibody
- TIMP1_HUMAN antibody
- Tissue Inhibitor of Metalloproteinase 1 antibody
- Tissue inhibitor of metalloproteinases 1 antibody
- Tissue inhibitor of metalloproteinases antibody
- Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor antibody
see all

Western blot - Anti-TIMP1 antibody [EPR18352] (ab211926)

All lanes : Anti-TIMP1 antibody [EPR18352] (ab211926) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TIMP1 knockout HeLa cell lysate
Lane 3 : HT-1080 treated with 200ng/ml 12-O Tetradecanoylphorbol-13-acetate (TPA) for 24 hours; cell lysate
Lane 4 : Untreated HT-1080 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 23 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab211926 observed at 26 kDa. Red - loading control ab7291 observed at 50 kDa.

ab211926 Anti-TIMP1 antibody [EPR18352] was shown to specifically react with TIMP1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261740 (knockout cell lysate ab257291) was used. Wild-type and TIMP1 knockout samples were subjected to SDS-PAGE. ab211926 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [EPR18352] (ab211926)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling TIMP1 with ab211926 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human lung cancer is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-TIMP1 antibody [EPR18352] (ab211926)

Anti-TIMP1 antibody [EPR18352] (ab211926) at 1/1000 dilution + Human prostate cancer lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 23 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?

Exposure time: 5 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 16517973).
Immunocytochemistry/ Immunofluorescence - Anti-TIMP1 antibody [EPR18352] (ab211926)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-OV-3 (Human ovarian cancer cell line) cells labeling TIMP1 with ab211926 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on SK-OV-3 cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows:
-ve control 1: ab211926 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Western blot - Anti-TIMP1 antibody [EPR18352] (ab211926)

All lanes : Anti-TIMP1 antibody [EPR18352] (ab211926) at 1/1000 dilution

Lane 1 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lane 2 : SK-OV-3 (Human ovarian cancer cell line) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 23 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.

Binding in these cell lines was extremely weak and requires optimization.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [EPR18352] (ab211926)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling TIMP1 with ab211926 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human colon neuroendocrine cell is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-TIMP1 antibody [EPR18352] (ab211926)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (Human colorectal adenocarcinoma cell line) cells labeling TIMP1 with ab211926 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HT-29 cell line. The nuclear counter stain is DAPI (blue).

Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

The negative controls are as follows:
-ve control 1: ab211926 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) (ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [EPR18352] (ab211926)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling TIMP1 with ab211926 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human islet is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [EPR18352] (ab211926)

Immunohistochemical analysis of paraffin-embedded human medullary thyroid carcinoma tissue labeling TIMP1 with ab211926 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human medullary thyroid carcinoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [EPR18352] (ab211926)

Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling TIMP1 with ab211926 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human prostate cancer is observed. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-TIMP1 antibody [EPR18352] (ab211926)

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

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Datasheet download

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发表研究结果有使用 ab211926？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab211926 被引用在 8 文献中.

Tan Y et al. TIMP1 down-regulation enhances gemcitabine sensitivity and reverses chemoresistance in pancreatic cancer. Biochem Pharmacol N/A:114085 (2020). PubMed: 32522594
Sun H & Feng X MicroRNA-367 directly targets PIK3R3 to inhibit proliferation and invasion of oral carcinoma cells. Biosci Rep 40:N/A (2020). PubMed: 32378714
Madjid TH et al. Expression of Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 in Endometriosis Menstrual Blood. Diagnostics (Basel) 10:N/A (2020). PubMed: 32498419
Xiang R et al. Different effects of allergic rhinitis on nasal mucosa remodeling in chronic rhinosinusitis with and without nasal polyps. Eur Arch Otorhinolaryngol 276:115-130 (2019). PubMed: 30446828
Shen DD et al. Liuweibuqi capsules improve pulmonary function in stable chronic obstructive pulmonary disease with lung-qi deficiency syndrome by regulating STAT4/STAT6 and MMP-9/TIMP-1. Pharm Biol 57:744-752 (2019). PubMed: 31679431
Cai J et al. Immune heterogeneity and clinicopathologic characterization of IGFBP2 in 2447 glioma samples. Oncoimmunology 7:e1426516 (2018). PubMed: 29721393
Abu El-Asrar AM et al. Differential expression and localization of human tissue inhibitors of metalloproteinases in proliferative diabetic retinopathy. Acta Ophthalmol 96:e27-e37 (2018). PubMed: 28391660
Han GJ et al. Experimental study of airway pressure release ventilation in the treatment of acute respiratory distress syndrome. Exp Ther Med 14:1941-1946 (2017). PubMed: 28962107

客户评价及客户问答

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1-9 of 9 Abreviews

Application

Immunohistochemistry (Frozen sections)

Sample

Human Tissue sections (Prostate cancer)

Permeabilization

Specification

Prostate cancer

Blocking step

Serum as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 21°C

Fixative

Acetone

Abcam user community

Verified customer

提交于 Dec 14 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Melanoma)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate

Permeabilization

Specification

Melanoma

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

提交于 Mar 27 2020

Application

Immunohistochemistry (Frozen sections)

Sample

Human Tissue sections (Melanoma)

Permeabilization

Specification

Melanoma

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Fixative

Acetone

Abcam user community

Verified customer

提交于 Mar 27 2020

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Melanoma cells)

Permeabilization

Specification

Melanoma cells

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Fixative

Acetone

Abcam user community

Verified customer

提交于 Mar 16 2020

Application

Immunocytochemistry

Sample

Human Cultured Cells (Melanoma cells)

Permeabilization

Specification

Melanoma cells

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Fixative

Acetone

Abcam user community

Verified customer

提交于 Mar 12 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Prostate)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: ab94681 Tris EDTA pH9.0

Permeabilization

Yes - 0.05% Tween 20

Specification

Prostate

Blocking step

Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C

Fixative

Formaldehyde

Abcam user community

Verified customer

提交于 Feb 26 2020

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Kidney H9 Organoids)

Permeabilization

Yes - 0.05% Tween 20

Specification

Kidney H9 Organoids

Blocking step

Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

提交于 Feb 11 2020

Application

Western blot

Sample

Human Cell lysate - whole cell (Hela, ARPE-19)

Gel Running Conditions

Reduced Denaturing (4-12% BisTris Gel)

Loading amount

10 µg

Treatment

IFNa 10 ng/ml, 6 hr

Specification

Hela, ARPE-19

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Aug 13 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Healthy prostate)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Sodium citrate, pH 6.0

Permeabilization

Specification

Healthy prostate

Blocking step

Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

Formaldehyde

Ms. S P

Verified customer

提交于 Mar 06 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

概述

产品名称

描述

宿主

Tested Applications & Species

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

Certificate of Compliance

文献 (8)

客户评价及客户问答

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Immunohistochemistry (Frozen sections) abreview for Anti-TIMP1 antibody [EPR18352]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TIMP1 antibody [EPR18352]

Immunohistochemistry (Frozen sections) abreview for Anti-TIMP1 antibody [EPR18352]

Immunocytochemistry/ Immunofluorescence abreview for Anti-TIMP1 antibody [EPR18352]

Immunocytochemistry abreview for Anti-TIMP1 antibody [EPR18352]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TIMP1 antibody [EPR18352]

Immunocytochemistry/ Immunofluorescence abreview for Anti-TIMP1 antibody [EPR18352]

Western blot abreview for Anti-TIMP1 antibody [EPR18352]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TIMP1 antibody [EPR18352]