Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1550] to TIMP1
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
参阅全部 TIMP1 一抗
描述兔单克隆抗体[EPR1550] to TIMP1
经测试应用适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC or IP
Synthetic peptide corresponding to Human TIMP1 aa 50-150.
- HeLa cell lysate; HT1080 + TPA, HL60+TPA, PBMC lysates, human prostate lysate; Human liver tissue; TIMP1 recombinant protein
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.05% BSA
Concentration information loading...
纯度Protein A purified
KO cell lines
Our Abpromise guarantee covers the use of ab109125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 23 kDa.|
|IHC-P||1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For unpurified use at 1/250 - 1/500.
功能Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
序列相似性Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
翻译后修饰The activity of TIMP1 is dependent on the presence of disulfide bonds.
- Information by UniProt
- Clgi antibody
- Collagenase inhibitor antibody
- Collagenase inhibitor, Human antibody
All lanes : Anti-TIMP1 antibody [EPR1550] (ab109125) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TIMP1 knockout HeLa cell lysate
Lane 3 : HT-1080 treated with 200ng/ml 12-O-Tetradecanoylphorbol-13-acetate (TPA) for 24 hours, cell lysate
Lane 4 : Untreated HT-1080 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Lanes 1-4: Merged signal (red and green). Green - ab109125 observed at 26 kDa. Red - loading control ab7291 observed at 50 kDa.
ab109125 Anti-TIMP1 antibody [EPR1550] was shown to specifically react with TIMP1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261740 (knockout cell lysate ab257291) was used. Wild-type and TIMP1 knockout samples were subjected to SDS-PAGE. ab109125 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostatic hyperplasia tissue sections labeling TIMP1 with purified ab109125 at 1:8000 dilution (0.21 μg/ml). Heat mediated antigen retrieval was performed using EDTA buffer, pH 9.0. Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid carcinoma tissue sections labeling TIMP1 with purified ab109125 at 1:8000 dilution (0.21 μg/ml). Heat mediated antigen retrieval was performed using EDTA buffer, pH 9.0. Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Unpurified ab109125, at 1/250 dilution staining TIMP1 in paraffin-embedded human liver tissue, by Immunohistochemistry.
All lanes : Anti-TIMP1 antibody [EPR1550] (ab109125) at 1/1000 dilution (purified)
Lane 1 : Human prostate lysates at 20 µg
Lane 2 : HT-1080 (Human fibrosarcoma epithelial cell) treated with 200ng/ml TPA for 24 hours whole cell lysates at 15 µg
Lane 3 : Untreated with HT-1080 (Human fibrosarcoma epithelial cell) whole cell lysates at 15 µg
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 23 kDa
Blocking and diluting buffer: 5% NFDM/TBST
All lanes : Anti-TIMP1 antibody [EPR1550] (ab109125) at 1/1000 dilution (unpurified)
Lane 1 : HL60+TPA cell lysates
Lane 2 : PBMC cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 23 kDa
Anti-TIMP1 antibody [EPR1550] (ab109125) at 1/1000 dilution (unpurified) + TIMP1 recombinant protein at 10 µg
Predicted band size: 23 kDa
ab109125 被引用在 12 文献中.
- Wang H et al. MicroRNA-96-5p promotes proliferation, invasion and EMT of oral carcinoma cells by directly targeting FOXF2. Biol Open 9:N/A (2020). PubMed: 32014885
- Ambhore NS et al. Differential estrogen-receptor activation regulates extracellular matrix deposition in human airway smooth muscle remodeling via NF-?B pathway. FASEB J 33:13935-13950 (2019). PubMed: 31638834
- Lan Y et al. OPN3 is a key regulator of UVA-induced photoaging in human dermal fibroblast cells. Br J Dermatol N/A:N/A (2019). PubMed: 31380578
- Zhang W et al. Knockdown of KDM1A suppresses tumour migration and invasion by epigenetically regulating the TIMP1/MMP9 pathway in papillary thyroid cancer. J Cell Mol Med 23:4933-4944 (2019). PubMed: 31211500
- Wu X et al. MicroRNA-663 participates in myocardial fibrosis through interaction with TGF-ß1. Exp Ther Med 18:3172-3176 (2019). PubMed: 31572558