Anti-TIMP1抗体[2A5] (ab2464)
Key features and details
- Mouse monoclonal [2A5] to TIMP1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-TIMP1抗体[2A5]
参阅全部 TIMP1 一抗 -
描述
小鼠单克隆抗体[2A5] to TIMP1 -
宿主
Mouse -
特异性
This monoclonal antibody binds to human TIMP-1. The antibody was tested for cross-reactivity with TIMP-2 and did not cross-react. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
不与反应: Mouse, Rat, Rabbit -
免疫原
Synthetic peptide:
FQALGDAADIR
, corresponding to amino acids 72-82 of Human TIMP1.(Murray et al., 1998) -
阳性对照
- Recombinant human TIMP1 protein (ab82104) can be used as a positive control in WB. Colon carcinoma.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
2A5 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab2464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | ||
| IHC-P |
WB: Use at an assay dependent concentration.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. -
序列相似性
Belongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain. -
翻译后修饰
The activity of TIMP1 is dependent on the presence of disulfide bonds. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 7076 Human
- Omim: 305370 Human
- SwissProt: P01033 Human
- Unigene: 522632 Human
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别名
- Clgi antibody
- Collagenase inhibitor antibody
- Collagenase inhibitor, Human antibody
see all
图片
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [2A5] (ab2464)](https://www.abcam.cn/ps/products/2/ab2464/Images/ab2464-274904-anti-timp1-antibody-2a5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIMP1 antibody [2A5] (ab2464)This image is courtesy of an anonymous abreview.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue sections labeling TIMP1 with undiluted ab2464. Tissue sections were fixed in formaldehyde; heat-mediated antigen retrieval was performed using a citrate buffer pH 6.0. The tissue was blocked with 3% serum for 30 minutes at 20°C, followed by incubation with Anti-TIMP1 antibody [2A5] (ab2464) for 12 hours at 20 °C in PBS. A biotin conjugate goat anti-mouse secondary antibody was used at 1/100 dilution.
数据表及文件
文献 (2)
ab2464 被引用在 2 文献中.
- Konno R et al. Gene expression profiling of the rat endometriosis model. Am J Reprod Immunol 58:330-43 (2007). PubMed: 17845203
- Murray GI et al. Matrix metalloproteinases and their inhibitors in gastric cancer. Gut 43:791-7 (1998). PubMed: 9824606
