Anti-TIMP1抗体[2A5] (ab2464)

Reviews (1)Q&A (1)References (3)

Key features and details

  • Mouse monoclonal [2A5] to TIMP1
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-TIMP1抗体[2A5]
    参阅全部 TIMP1 一抗

  • 描述

    小鼠单克隆抗体[2A5] to TIMP1

  • 宿主

    Mouse

  • 特异性

    This monoclonal antibody binds to human TIMP-1. The antibody was tested for cross-reactivity with TIMP-2 and did not cross-react.

  • 种属反应性

    与反应: Human
    不与反应: Mouse, Rat, Rabbit

  • 免疫原

    Synthetic peptide:

    FQALGDAADIR

    , corresponding to amino acids 72-82 of Human TIMP1.(Murray et al., 1998)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

    • Recombinant human TIMP1 protein (ab82104) can be used as a positive control in WB. Colon carcinoma.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

  • 应用说明
    IHC-P: Use at an assay dependent concentration. Perform antigen retrieval: microwave 7 min 950W or 20 min 800W in 0.01 M sodium citrate buffer pH 6.0.
    WB: Use at an assay dependent concentration.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • 靶标

    • 功能

      Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.

    • 序列相似性

      Belongs to the protease inhibitor I35 (TIMP) family.
      Contains 1 NTR domain.

    • 翻译后修饰

      The activity of TIMP1 is dependent on the presence of disulfide bonds.

    • 细胞定位

      Secreted.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Clgi antibody
        • Collagenase inhibitor antibody
        • Collagenase inhibitor, Human antibody
        • EPA antibody
        • EPO antibody
        • Erythroid Potentiating Activity antibody
        • Erythroid-potentiating activity antibody
        • Fibroblast collagenase inhibitor antibody
        • FLJ90373 antibody
        • HCI antibody
        • Human Collagenase Inhibitor antibody
        • Metalloproteinase inhibitor 1 antibody
        • Metalloproteinase inhibitor 1 precursor antibody
        • OTTHUMP00000023214 antibody
        • TIMP 1 antibody
        • TIMP antibody
        • TIMP metallopeptidase inhibitor 1 antibody
        • TIMP-1 antibody
        • Timp1 antibody
        • TIMP1 protein antibody
        • TIMP1_HUMAN antibody
        • Tissue Inhibitor of Metalloproteinase 1 antibody
        • Tissue inhibitor of metalloproteinases 1 antibody
        • Tissue inhibitor of metalloproteinases antibody
        • Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor antibody
        see all

      文献 (3)

      发表研究结果有使用 ab2464？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab2464 被引用在 3 文献中.

      • Rong X  et al. Antler stem cells as a novel stem cell source for reducing liver fibrosis. Cell Tissue Res 379:195-206 (2020). PubMed: 31428875
      • Konno R  et al. Gene expression profiling of the rat endometriosis model. Am J Reprod Immunol 58:330-43 (2007). PubMed: 17845203
      • Murray GI  et al. Matrix metalloproteinases and their inhibitors in gastric cancer. Gut 43:791-7 (1998). PubMed: 9824606

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      1-2 of 2 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TIMP1 antibody [2A5]

       Good
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (lung)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6,0
      Specification
      lung
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
      Fixative
      Formaldehyde
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      Abcam user community

      Verified customer

      提交于 Jan 27 2017

      Question

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      Answer

      Thank you for your enquiry. I have contacted the source of this antibody and unfortunately the concentration has not been determined. The starting dilution for IHC should be 1:2, in addition to serial dilutions to determine the optimal concentration. I have also discovered that antigen retrieval (microwave 7 min 950W or 20 min 800W in 0.01 M sodium citrate buffer pH 6.0) should be performed. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

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