Anti-TIAL1抗体(ab129499)
Key features and details
- Rabbit polyclonal to TIAL1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-TIAL1抗体
参阅全部 TIAL1 一抗 -
描述
兔多克隆抗体to TIAL1 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus
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免疫原
Synthetic peptide, corresponding to a region within amino acids 325-375 of Human TIAL1 (NP_003243.1).
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阳性对照
- HeLa, 293T, and Jurkat whole cell lysates.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab129499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 41 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
靶标
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功能
RNA-binding protein. Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis. -
序列相似性
Contains 3 RRM (RNA recognition motif) domains. -
翻译后修饰
Phosphorylated by MAPK14 following DNA damage, releasing TIAR from GADD45A mRNA. -
细胞定位
Cytoplasm. Nucleus. Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). Cytoplasmic granules of cytolytic T-lymphocytes. - Information by UniProt
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数据库链接
- Entrez Gene: 771327 Chicken
- Entrez Gene: 614540 Cow
- Entrez Gene: 477841 Dog
- Entrez Gene: 101143159 Gorilla
- Entrez Gene: 100058604 Horse
- Entrez Gene: 7073 Human
- Entrez Gene: 21843 Mouse
- Entrez Gene: 100158186 Pig
see all -
别名
- Aging associated gene 7 protein antibody
- Nucleolysin TIAR antibody
- T cluster binding protein antibody
see all
图片
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Western blot - Anti-TIAL1 antibody (ab129499)All lanes : Anti-TIAL1 antibody (ab129499) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-TIAL1 antibody (ab129499)Detection of TIAL1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab129499 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
Detection: Chemiluminescence with an exposure time of 30 seconds.
数据表及文件
文献
ab129499 被引用在 1 文献中.
- Wang Q et al. miR-223-3p/TIAL1 interaction is involved in the mechanisms associated with the neuroprotective effects of dexmedetomidine on hippocampal neuronal cells in vitro. Mol Med Rep 19:805-812 (2019). PubMed: 30569136
