Anti-TIAL1抗体(ab129499)

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Western blot - Anti-TIAL1 antibody (ab129499)
  • Immunoprecipitation - Anti-TIAL1 antibody (ab129499)

Key features and details

  • Rabbit polyclonal to TIAL1
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-TIAL1抗体
    参阅全部 TIAL1 一抗

  • 描述

    兔多克隆抗体to TIAL1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IPmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus

  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 325-375 of Human TIAL1 (NP_003243.1).

  • 阳性对照

    • HeLa, 293T, and Jurkat whole cell lysates.

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

应用

Our Abpromise guarantee covers the use of ab129499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
IP Use at 2-10 µg/mg of lysate.

靶标

图片

  • Western blot - Anti-TIAL1 antibody (ab129499)
    Western blot - Anti-TIAL1 antibody (ab129499)
    All lanes : Anti-TIAL1 antibody (ab129499) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 41 kDa


    Exposure time: 30 seconds
  • Immunoprecipitation - Anti-TIAL1 antibody (ab129499)
    Immunoprecipitation - Anti-TIAL1 antibody (ab129499)
    Detection of TIAL1 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab129499 at 6 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
    Detection: Chemiluminescence with an exposure time of 30 seconds.

文献 (1)

发表研究结果有使用 ab129499？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab129499 被引用在 1 文献中.

  • Wang Q  et al. miR-223-3p/TIAL1 interaction is involved in the mechanisms associated with the neuroprotective effects of dexmedetomidine on hippocampal neuronal cells in vitro. Mol Med Rep 19:805-812 (2019). PubMed: 30569136

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