Anti-TIA1抗体[TIA-1] (ab2712)

Reviews (6)Q&A (5)References (16)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [TIA-1] (ab2712)

    Key features and details

    • Mouse monoclonal [TIA-1] to TIA1
    • Suitable for: IHC-P
    • Reacts with: Mouse, Human, Monkey
    • Isotype: IgG1

    概述

    • 产品名称

      Anti-TIA1抗体[TIA-1]
      参阅全部 TIA1 一抗

    • 描述

      小鼠单克隆抗体[TIA-1] to TIA1

    • 宿主

      Mouse

    • 经测试应用

      适用于: IHC-Pmore details

    • 种属反应性

      与反应: Mouse, Human, Monkey

    • 免疫原

      Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

    • 阳性对照

      • IHC-P: Human spleen tissue.

    • 常规说明

      TIA-1 (T-cell intracytoplasmic antigen) monoclonal antibody reacts with a 15 kDa cytoplasmic granule-associated protein, expressed in lymphocytes processing cytolytic potential. The expression of TIA-1 was studied in CD30+ anaplastic large cell lymphomas (ALCL), NK cell lymphomas and peripheral T-cell lymphomas and T cell lymphocytosis, B-cell lymphomas and lymphoblastic leukemia, Hodgkin’s etc. Studies showed that 60 to 70% of anaplastic large cell lymphoma reacted with TIA-1. Studies also indicate that TIA-1 reacts with most large granular lymphocytic leukemia’s, hepatosplenic T-cell lymphomas, intestinal T-cell lymphomas, NK-like T-cell lymphomas, NK-cell lymphomas, nasal T/NK-cell lymphomas, subcutaneous T-cell lymphomas, pulmonary angiocentric lymphomas of T or NK phenotype. The author’s concluded from TIA-1 studies that anaplastic large-cell lymphomas are cytotoxic T-or NK-cell neoplasms. All B-cell lymphomas, Hodgkin’s and lymphoblastic leukemias were negative for TIA-1.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • 存储溶液

      Preservative: 0.1% Sodium azide
      Constituent: Proprietary Solution
    • Concentration information loading...

    • 纯度

      Proprietary Purification

    • Primary antibody说明

      TIA-1 (T-cell intracytoplasmic antigen) monoclonal antibody reacts with a 15 kDa cytoplasmic granule-associated protein, expressed in lymphocytes processing cytolytic potential. The expression of TIA-1 was studied in CD30+ anaplastic large cell lymphomas (ALCL), NK cell lymphomas and peripheral T-cell lymphomas and T cell lymphocytosis, B-cell lymphomas and lymphoblastic leukemia, Hodgkin’s etc. Studies showed that 60 to 70% of anaplastic large cell lymphoma reacted with TIA-1. Studies also indicate that TIA-1 reacts with most large granular lymphocytic leukemia’s, hepatosplenic T-cell lymphomas, intestinal T-cell lymphomas, NK-like T-cell lymphomas, NK-cell lymphomas, nasal T/NK-cell lymphomas, subcutaneous T-cell lymphomas, pulmonary angiocentric lymphomas of T or NK phenotype. The author’s concluded from TIA-1 studies that anaplastic large-cell lymphomas are cytotoxic T-or NK-cell neoplasms. All B-cell lymphomas, Hodgkin’s and lymphoblastic leukemias were negative for TIA-1.

    • 克隆

      单克隆

    • 克隆编号

      TIA-1

    • 骨髓瘤

      unknown

    • 同种型

      IgG1

    • 轻链类型

      unknown

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab2712于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    IHC-P (1)
    1/50 - 1/100. Incubate for 30-60 minutes at RT. Use heat-mediated antigen retrieval, an optional pepsin treatment can also improve the signal.
    说明
    IHC-P
    1/50 - 1/100. Incubate for 30-60 minutes at RT. Use heat-mediated antigen retrieval, an optional pepsin treatment can also improve the signal.

    靶标

    • 功能

      Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.

    • 序列相似性

      Contains 3 RRM (RNA recognition motif) domains.

    • 细胞定位

      Cytoplasmic granule. Nucleus. Accumulates in cytoplasmic stress granules (SG) following cellular damage.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Cytotoxic granule associated RNA binding protein 1 antibody
        • Cytotoxic granule associated RNA binding protein antibody
        • mTIA-1 antibody
        • Nucleolysin TIA 1 isoform p40 antibody
        • Nucleolysin TIA-1 isoform p40 antibody
        • Nucleolysin TIA1 isoform p40 antibody
        • p40 TIA 1 antibody
        • p40-TIA-1 (containing p15-TIA-1) antibody
        • p40-TIA-1 antibody
        • RNA binding protein TIA 1 antibody
        • RNA binding protein TIA1 antibody
        • RNA-binding protein TIA-1 antibody
        • T-cell-restricted intracellular antigen-1 antibody
        • TIA 1 antibody
        • TIA 1 cytotoxic granule associated RNA binding protein antibody
        • Tia antibody
        • TIA-1 antibody
        • TIA1 antibody
        • TIA1 cytotoxic granule associated RNA binding protein antibody
        • TIA1 cytotoxic granule associated RNA binding protein like 1 antibody
        • TIA1 protein antibody
        • TIA1_HUMAN antibody
        • TIAL1 antibody
        • TIAR antibody
        • WDM antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [TIA-1] (ab2712)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIA1 antibody [TIA-1] (ab2712)

        Human normal spleen. Staining is observed in the cytoplasm. Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control. Sections were stained using an automated system at room temperature: sections were rehydrated and antigen retrieved with buffers citrate EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked for 10 minutes (casein 0.25% in PBS) then incubated with primary antibody for 20 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

      文献 (16)

      发表研究结果有使用 ab2712？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab2712 被引用在 16 文献中.

      • Lennox AL  et al. Pathogenic DDX3X Mutations Impair RNA Metabolism and Neurogenesis during Fetal Cortical Development. Neuron 106:404-420.e8 (2020). PubMed: 32135084
      • Niu Z  et al. Myopathy With SQSTM1 and TIA1 Variants: Clinical and Pathological Features. Front Neurol 9:147 (2018). IHC-P ; Human . PubMed: 29599744
      • Ghosh S & Geahlen RL Stress Granules Modulate SYK to Cause Microglial Cell Dysfunction in Alzheimer's Disease. EBioMedicine 2:1785-98 (2015). WB, ICC/IF ; Mouse . PubMed: 26870803
      • van Gool IC  et al. POLE Proofreading Mutations Elicit an Antitumor Immune Response in Endometrial Cancer. Clin Cancer Res 21:3347-55 (2015). IHC-P ; Human . PubMed: 25878334
      • Tessier SN  et al. The involvement of mRNA processing factors TIA-1, TIAR, and PABP-1 during mammalian hibernation. Cell Stress Chaperones 19:813-25 (2014). PubMed: 24590458
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TIA1 antibody [TIA-1]

       Excellent
      Abreviews
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Nasopharyngeal carcinoma)
      Specification
      Nasopharyngeal carcinoma
      Fixative
      Formaldehyde
      Antigen retrieval step
      Heat mediated
      Blocking step
      Serum as blocking agent for 10 minute(s) · Concentration: 5%
      Read More

      Mr. Chit Chow

      Verified customer

      提交于 Nov 23 2005

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com