重组Anti-TIA1抗体[EPR9304] - BSA and Azide free (ab230829)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9304] to TIA1 - BSA and Azide free
- Suitable for: IP, ICC/IF, IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-TIA1抗体[EPR9304] - BSA and Azide free
参阅全部 TIA1 一抗 -
描述
兔单克隆抗体[EPR9304] to TIA1 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: IP, ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HeLa, HuT-78, Jurkat, Molt4, NIH/3T3 and K562 cell lysates. IHC-P: Human spleen tissue. ICC/IF: HuT-78 cells. IP: HuT-78 cells.
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常规说明
ab230829 is the carrier-free version of ab140595.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR9304 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-TIA1 antibody [EPR9304] (ab140595)
- Alexa Fluor® 488 Anti-TIA1 antibody [EPR9304] (ab196382)
- Alexa Fluor® 647 Anti-TIA1 antibody [EPR9304] (ab311116)
- Alexa Fluor® 594 Anti-TIA1 antibody [EPR9304] (ab311742)
- Alexa Fluor® 568 Anti-TIA1 antibody [EPR9304] (ab313022)
- Alexa Fluor® 555 Anti-TIA1 antibody [EPR9304] (ab313224)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab230829于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.
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说明 |
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IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa. |
靶标
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功能
Involved in alternative pre-RNA splicing and regulation of mRNA translation by binding to AU-rich elements (AREs) located in mRNA 3' untranslated regions (3' UTRs). Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis. -
序列相似性
Contains 3 RRM (RNA recognition motif) domains. -
细胞定位
Cytoplasmic granule. Nucleus. Accumulates in cytoplasmic stress granules (SG) following cellular damage. - Information by UniProt
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数据库链接
- Entrez Gene: 7072 Human
- Entrez Gene: 21841 Mouse
- Omim: 603518 Human
- SwissProt: P31483 Human
- SwissProt: P52912 Mouse
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别名
- Cytotoxic granule associated RNA binding protein 1 antibody
- Cytotoxic granule associated RNA binding protein antibody
- mTIA-1 antibody
see all
图片
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Immunocytochemistry/Immunofluorescence analysis of HuT-78 cells labelling TIA1 with purified ab140595 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140595).
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This IHC data was generated using the same anti-TIA1 antibody clone, EPR9304, in a different buffer formulation (cat# ab140595).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling TIA1 with purified ab140595 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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This WB data was generated using the same anti-TIA1 antibody clone, EPR9304, in a different buffer formulation (cat# ab140595).
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: TIA1 knockout HAP1 cell lysate (40 µg)
Lane 3: Jurkat cell lysate (40 µg)
Lane 4: K562 cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab140595 observed at 43 kDa. Red - loading control, ab18058, observed at 124 kDa.ab140595 was shown to specifically react with TIA1 when TIA1 knockout samples were used. Wild-type and TIA1 knockout samples were subjected to SDS-PAGE. Ab140595 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling TIA1 with unpurified ab140595 at a dilution of 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140595).
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ab140595 (purified) at 1/40 immunoprecipitating TIA1 in HuT-78 whole cell lysate.
Lane 1 (input): HuT-78 whole cell lysate (10µg)
Lane 2 (+): ab140595 + HuT-78 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab140595 in HuT-78 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab140595).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab230829 尚未被引用在任何文献中。