Anti-Thyroid Hormone Receptor beta抗体(ab5622)
Key features and details
- Rabbit polyclonal to Thyroid Hormone Receptor beta
- Suitable for: ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Thyroid Hormone Receptor beta抗体
参阅全部 Thyroid Hormone Receptor beta 一抗 -
描述
兔多克隆抗体to Thyroid Hormone Receptor beta -
宿主
Rabbit -
特异性
This antibody does not detect TR alpha-1 or TRv alpha-2. -
经测试应用
适用于: ICC/IF, IHC-Pmore details -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide corresponding to Human Thyroid Hormone Receptor beta aa 62-81. With an N-terminal added cysteine.
Sequence:IFHLDHDDVNDQSVSSAQTF
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阳性对照
- IHC-P: Human colon, Human thyroid, Rat thyroid tissues. ICC: HeLa, A431 and L6 cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
1/50 - 1/500.
|
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| IHC-P |
1/100 - 1/500.
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| 说明 |
|---|
|
ICC/IF
1/50 - 1/500. |
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IHC-P
1/100 - 1/500. |
靶标
-
功能
High affinity receptor for triiodothyronine. -
疾病相关
Defects in THRB are the cause of generalized thyroid hormone resistance (GTHR) [MIM:188570, 274300]. GTHR is transmitted as an autosomal dominant trait, but an autosomal recessive form also exists. The disease is characterized by goiter, abnormal mental functions, increased susceptibility to infections, abnormal growth and bone maturation, tachycardia and deafness. Affected individuals may also have attention deficit-hyperactivity disorders (ADHD) and language difficulties. GTHR patients also have high levels of circulating thyroid hormones (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH).
Defects in THRB are the cause of selective pituitary thyroid hormone resistance (PRTH) [MIM:145650]; also known as familial hyperthyroidism due to inappropriate thyrotropin secretion. PRTH is a variant form of thyroid hormone resistance and is characterized by clinical hyperthyroidism, with elevated free thyroid hormones, but inappropriately normal serum TSH. Unlike GRTH, where the syndrome usually segregates with a dominant allele, the mode of inheritance in PRTH has not been established. -
序列相似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
结构域
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 7068 Human
- Entrez Gene: 24831 Rat
- Omim: 190160 Human
- SwissProt: P10828 Human
- SwissProt: P18113 Rat
- Unigene: 187861 Human
- Unigene: 728126 Human
- Unigene: 88692 Rat
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别名
- Avian erythroblastic leukemia viral (v erb a) oncogene homolog 2 antibody
- C ERBA 2 antibody
- C ERBA BETA antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)
ab5622 labelling Thyroid Hormaone Receptor beta in the nucleus of Rat thyroid tissue (right) compared with a negative control (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)ab5622 labelling Thyroid Hormaone Receptor beta in the nucleus of Human thyroid tissue (right) compared with a negative control (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)ab5622 staining Thyroid Hormone Receptor in Human colon tissue sections (right) compared to negative control (left) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% H2O2-methanol for 15 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/20) for 1 hour at 37°C. A HRP-conjugated secondary antibody was used for detection.
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Immunocytochemistry/ Immunofluorescence - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)ab5622 staining Thyroid Hormone Receptor beta in A431 cells (right) compared to negative control (left) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with forrmalin, permeabilized with 0.1% Triton X-100 in TBS and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100) overnight at 4°C. A Dylight-conjugated secondary antibody was used. F-actin stained with red phallodin (red) and nuclei stained with Hoechst (blue).
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Immunocytochemistry/ Immunofluorescence - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)ab5622 staining Thyroid Hormone Receptor beta in Hela cells (right) compared to negative control (left) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with forrmalin, permeabilized with 0.1% Triton X-100 in TBS and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100) overnight at room temperature. A Dylight-conjugated secondary antibody was used. F-actin stained with red phallodin (red) and nuclei stained with Hoechst (blue).
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Immunocytochemistry/ Immunofluorescence - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)ab5622 staining Thyroid Hormone Receptor beta in L6 cells (right) compared to negative control (left) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with forrmalin, permeabilized with 0.1% Triton X-100 in TBS and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100) overnight at 4°C. A Dylight-conjugated secondary antibody was used. F-actin stained with red phallodin (red) and nuclei stained with Hoechst (blue).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (15)
ab5622 被引用在 15 文献中.
- Tang Y et al. Increased GABAergic development in iPSC-derived neurons from patients with sporadic Alzheimer's disease. Neurosci Lett 735:135208 (2020). PubMed: 32615251
- Pörings AS et al. A thyroid hormone network exists in synovial fibroblasts of rheumatoid arthritis and osteoarthritis patients. Sci Rep 9:13235 (2019). PubMed: 31519956
- Harde E et al. EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development. Elife 8:N/A (2019). PubMed: 31868584
- Mahaman YAR et al. Moringa Oleifera Alleviates Homocysteine-Induced Alzheimer's Disease-Like Pathology and Cognitive Impairments. J Alzheimers Dis 63:1141-1159 (2018). PubMed: 29710724
- Yakoub AM & Sadek M Development and Characterization of Human Cerebral Organoids: An Optimized Protocol. Cell Transplant 27:393-406 (2018). PubMed: 29749250