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Signal Transduction Growth Factors/Hormones Hormones

Anti-Thyroid Hormone Receptor beta抗体(ab5622)

  • Datasheet
  • SDS
Submit a review Q&A (6)References (22)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)
  • Immunocytochemistry/ Immunofluorescence - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)
  • Immunocytochemistry/ Immunofluorescence - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)
  • Immunocytochemistry/ Immunofluorescence - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)

Key features and details

  • Rabbit polyclonal to Thyroid Hormone Receptor beta
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG

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Recombinant Human Thyroid Hormone Receptor beta protein (ab82049)

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概述

  • 产品名称

    Anti-Thyroid Hormone Receptor beta抗体
    参阅全部 Thyroid Hormone Receptor beta 一抗
  • 描述

    兔多克隆抗体to Thyroid Hormone Receptor beta
  • 宿主

    Rabbit
  • 特异性

    This antibody does not detect TR alpha-1 or TRv alpha-2.
  • 经测试应用

    适用于: ICC/IF, IHC-Pmore details
  • 种属反应性

    与反应: Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Thyroid Hormone Receptor beta aa 62-81. With an N-terminal added cysteine.
    Sequence:

    IFHLDHDDVNDQSVSSAQTF

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • IHC-P: Human colon, Human thyroid, Rat thyroid tissues. ICC: HeLa, A431 and L6 cells.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度

    Whole antiserum
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Neuroscience
    • Endocrine system
    • Thyroid axis

相关产品

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Thyroid Hormone Receptor beta protein (ab82049)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5622于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/50 - 1/500.
IHC-P
1/100 - 1/500.
说明
ICC/IF
1/50 - 1/500.
IHC-P
1/100 - 1/500.

靶标

  • 功能

    High affinity receptor for triiodothyronine.
  • 疾病相关

    Defects in THRB are the cause of generalized thyroid hormone resistance (GTHR) [MIM:188570, 274300]. GTHR is transmitted as an autosomal dominant trait, but an autosomal recessive form also exists. The disease is characterized by goiter, abnormal mental functions, increased susceptibility to infections, abnormal growth and bone maturation, tachycardia and deafness. Affected individuals may also have attention deficit-hyperactivity disorders (ADHD) and language difficulties. GTHR patients also have high levels of circulating thyroid hormones (T3-T4), with normal or slightly elevated thyroid stimulating hormone (TSH).
    Defects in THRB are the cause of selective pituitary thyroid hormone resistance (PRTH) [MIM:145650]; also known as familial hyperthyroidism due to inappropriate thyrotropin secretion. PRTH is a variant form of thyroid hormone resistance and is characterized by clinical hyperthyroidism, with elevated free thyroid hormones, but inappropriately normal serum TSH. Unlike GRTH, where the syndrome usually segregates with a dominant allele, the mode of inheritance in PRTH has not been established.
  • 序列相似性

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 细胞定位

    Nucleus.
  • Target information above from: UniProt accession P10828 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 7068 Human
    • Entrez Gene: 24831 Rat
    • Omim: 190160 Human
    • SwissProt: P10828 Human
    • SwissProt: P18113 Rat
    • Unigene: 187861 Human
    • Unigene: 728126 Human
    • Unigene: 88692 Rat
    • 别名

      • Avian erythroblastic leukemia viral (v erb a) oncogene homolog 2 antibody
      • C ERBA 2 antibody
      • C ERBA BETA antibody
      • c-erbA-2 antibody
      • c-erbA-beta antibody
      • ERBA 2 antibody
      • ERBA BETA antibody
      • ERBA2 antibody
      • Erythroblastic leukemia viral (v erb a) oncogene homolog 2 avian antibody
      • generalized resistance to thyroid hormone antibody
      • GRTH antibody
      • MGC126109 antibody
      • MGC126110 antibody
      • NR1A2 antibody
      • Nuclear receptor subfamily 1 group A member 2 antibody
      • Oncogene ERBA2 antibody
      • PRTH antibody
      • THB_HUMAN antibody
      • THR1 antibody
      • THRB 1 antibody
      • THRB 2 antibody
      • thrB antibody
      • THRB1 antibody
      • THRB2 antibody
      • Thyroid hormone nuclear receptor beta variant 1 antibody
      • Thyroid hormone receptor beta 1 antibody
      • Thyroid hormone receptor beta 2 antibody
      • Thyroid hormone receptor beta antibody
      • Thyroid hormone receptor, beta (erythroblastic leukemia viral (v erb a) oncogene homolog 2, avian) antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)

      ab5622 labelling Thyroid Hormaone Receptor beta in the nucleus of Rat thyroid tissue (right) compared with a negative control (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)

      ab5622 labelling Thyroid Hormaone Receptor beta in the nucleus of Human thyroid tissue (right) compared with a negative control (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)

      ab5622 staining Thyroid Hormone Receptor in Human colon tissue sections (right) compared to negative control (left) by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 3% H2O2-methanol for 15 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/20) for 1 hour at 37°C. A HRP-conjugated secondary antibody was used for detection.

    • Immunocytochemistry/ Immunofluorescence - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)
      Immunocytochemistry/ Immunofluorescence - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)

      ab5622 staining Thyroid Hormone Receptor beta in A431 cells (right) compared to negative control (left) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with forrmalin, permeabilized with 0.1% Triton X-100 in TBS and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100) overnight at 4°C. A Dylight-conjugated secondary antibody was used. F-actin stained with red phallodin (red) and nuclei stained with Hoechst (blue).

    • Immunocytochemistry/ Immunofluorescence - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)
      Immunocytochemistry/ Immunofluorescence - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)

      ab5622 staining Thyroid Hormone Receptor beta in Hela cells (right) compared to negative control (left) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with forrmalin, permeabilized with 0.1% Triton X-100 in TBS and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100) overnight at room temperature. A Dylight-conjugated secondary antibody was used. F-actin stained with red phallodin (red) and nuclei stained with Hoechst (blue).

    • Immunocytochemistry/ Immunofluorescence - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)
      Immunocytochemistry/ Immunofluorescence - Anti-Thyroid Hormone Receptor beta antibody - ChIP Grade (ab5622)

      ab5622 staining Thyroid Hormone Receptor beta in L6 cells (right) compared to negative control (left) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with forrmalin, permeabilized with 0.1% Triton X-100 in TBS and blocked with 3% BSA for 30 minutes at room temperature. Samples were incubated with primary antibody (1/100) overnight at 4°C. A Dylight-conjugated secondary antibody was used. F-actin stained with red phallodin (red) and nuclei stained with Hoechst (blue).

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (22)

    发表研究结果有使用 ab5622?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5622 被引用在 22 文献中.

    • Ping S  et al. Brain-derived CCR5 Contributes to Neuroprotection and Brain Repair after Experimental Stroke. Aging Dis 12:72-92 (2021). PubMed: 33532129
    • Chen X  et al. Thyroid Hormone-Regulated Expression of Period2 Promotes Liver Urate Production. Front Cell Dev Biol 9:636802 (2021). PubMed: 33869182
    • Teoh J  et al. Arfgef1 haploinsufficiency in mice alters neuronal endosome composition and decreases membrane surface postsynaptic GABAA receptors. Neurobiol Dis 134:104632 (2020). PubMed: 31678406
    • Hathy E  et al. Investigation of de novo mutations in a schizophrenia case-parent trio by induced pluripotent stem cell-based in vitro disease modeling: convergence of schizophrenia- and autism-related cellular phenotypes. Stem Cell Res Ther 11:504 (2020). PubMed: 33246498
    • Tang Y  et al. Increased GABAergic development in iPSC-derived neurons from patients with sporadic Alzheimer's disease. Neurosci Lett 735:135208 (2020). PubMed: 32615251
    View all Publications for this product

    客户评价及客户问答

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    1-6 of 6 Abreviews or Q&A

    Question

    I talked to my adviser and she would like accept your offer.
    We would like to try the western blot again with ab53170.
    Can you send it to us?
    What do you need besides this address below my name?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 08 2012

    Answer

    Thank you for your email.
    Yes, I will arrange for the shipment via the distributor through which the antibody ab5622 was originally purchased.

    On the original order I saw that 2 vials had been ordered. Could you please let me know whether you had ordered 2 or just 1 vial?
    In case you had ordered2 vials, would you like me to send you 2 vials of ab53170?

    Please let me know and I will arrange for everything.

    I look forward to hear back from you.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    回复于 Aug 08 2012

    Question

    My samples are from human HEK 293 cells.
    I don't know what is the order number, but I remember that the antibody arrived around xxx. And the Lot number is GR49085-1.
    I tried to use only the secondary antibody and i had no luminescence at all. The film was blank.
    What can you do for me?
    I think the problem is your primary antibody, maybe it has expired.

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    Abcam community

    Verified customer

    Asked on Jul 25 2012

    Answer

    Thank you for the additional information.

    I found an order from xxx. Can you please confirm that you ordered the antibody via xxx?

    Our guarantee is valid only for 6 months after the purchase (https://www.abcam.com/Abpromise). But I'd be happy to make a one-time exception and can offer you either a free of charge replacement with another antibody (e.g. Thyroid-Hormone-Receptor-beta-antibody-ab106813.html, Thyroid-Hormone-Receptor-beta-antibody-ab53170.htmlor Thyroid-Hormone-Receptor-beta-antibody-ab33544.html)or a credit.

    Please let me know which option would be best for you.

    I look forward to hear back.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Abcam Scientific Support

    回复于 Jul 25 2012

    Question

    Product code: 5622
    Lot number: GR49085-1
    Inquiry: WB: 100ug of total protein extract
    SDS-Page Gel 10%
    Wet Transfer 2h, 300mA 2 hour
    blocking with milk
    Primary antibody anti-TRbeta 1:1000 (TBS-T) OverNight 4oC
    Wash 2x with TBS-T
    Second antibody anti-Rabbit 1:5000 4oC, 2h
    Wash 3x 10min with TBS-T
    Detection method - ECL Prime (GE)
    Following this protocol I haven't been able to detect clear bands, i see only a lot of background, even with an exposure of 3 seconds. I don't think that the antibody is working.

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    Abcam community

    Verified customer

    Asked on Jul 04 2012

    Answer

    Thank you for contacting us.

    I am sorry to hear that the antibody is not working very well.

    Could you please help me answer a few more questions:
    1) What samples are you using and which species are they from?
    2) Have you tried a no primary control to see if the secondary antibody contributes to or causes the high background?
    3) When did you order the antibody and what is your order number?

    I look forward to hear back from you and assist you further.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Abcam Scientific Support

    回复于 Jul 04 2012

    Question

    Customer called because they had been attempting to use our products ab5622 and ab106813 in WB on human and rat cell lines but had experiences NSB.  They attempted many troubleshooting steps but  were unable to resolve the issue. They have found another product which does work in there assay and ask for a credit.

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    Abcam community

    Verified customer

    Asked on May 22 2012

    Answer

    Thank you for contacting us. Your credit note IDs are XXX. I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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    Abcam Scientific Support

    回复于 May 22 2012

    Question

    The datasheet says "This antibody does not detect TR alpha-1 or TRv alpha-2." but also says " By Western blot, this antibody detects a 55 kDa protein representing recombinant human TRv alpha-2 expressed in E. coli." Is this a datasheet error?

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    Abcam community

    Verified customer

    Asked on Feb 01 2006

    Answer

    I can confirm that the sentence regarding TRv alpha-2 was a datasheet error. Thank you for bringing this to our attention. The information should read, "this antibody detects a 55 kDa protein representing recombinant human TRv Beta-1 expressed in E. coli." and it has been corrected immediately.

    Read More

    Abcam Scientific Support

    回复于 Feb 02 2006

    Question

    I just left a voice message with technical service. Below is the specific question that I have. There are several TR alpha and TR beta specific antibodies that you sell. I was wondering if you also sell the peptides for them. The catalog numbers of the antibodies are: ab5621 ab15544 ab3288 ab5622 ab15545 ab2743 ab2744

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    Abcam community

    Verified customer

    Asked on Dec 09 2004

    Answer

    Thank you for your enquiry. Unfortunately, we don't have the corresponding peptides for these antibodies. If you cannot find what you are looking for within the Abcam catalogue, you can click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogues of all online antibody companies (currently 249). Should you require any further information, please get in touch with us and we will gladly assist you.

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    Abcam Scientific Support

    回复于 Dec 09 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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