重组Anti-Thyroglobulin抗体[EPR9730] (ab156008)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9730] to Thyroglobulin
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Thyroglobulin抗体[EPR9730]
参阅全部 Thyroglobulin 一抗 -
描述
兔单克隆抗体[EPR9730] to Thyroglobulin -
宿主
Rabbit -
特异性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. -
经测试应用
适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human Thyroglobulin aa 1000-1100. The exact sequence is proprietary.
Database link: P01266 -
阳性对照
- Mouse thyroid, rat thyroid, and Human thyroid lysates, Human thyroid gland follicular carcinoma tissue and Human thyroid gland papillary carcinoma tissue, TT cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR9730 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-Thyroglobulin antibody [EPR9730(IHC)] (ab168344)
- Alexa Fluor® 488 Anti-Thyroglobulin antibody [EPR9730] (ab214872)
- APC Anti-Thyroglobulin antibody [EPR9730] (ab221284)
- Anti-Thyroglobulin antibody [EPR9730] - Low endotoxin, Azide free (ab229449)
- Anti-Thyroglobulin antibody [EPR9730(IHC)] - BSA and Azide free (ab249444)
- Anti-Thyroglobulin antibody [EPR9730] - BSA and Azide free (ab271919)
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab156008于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB |
1/10000 - 1/50000. Detects a band of approximately 294-300 kDa (predicted molecular weight: 305 kDa).
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
|
ICC/IF |
1/50 - 1/100.
|
说明 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
1/10000 - 1/50000. Detects a band of approximately 294-300 kDa (predicted molecular weight: 305 kDa). |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
ICC/IF
1/50 - 1/100. |
靶标
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功能
Precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). -
组织特异性
Thyroid gland specific. -
疾病相关
Defects in TG are the cause of congenital hypothyroidism due to dyshormonogenesis type 3 (CHDH3) [MIM:274700]. A disorder due to thyroid dyshormonogenesis, causing large goiters of elastic and soft consistency in the majority of patients. Although the degree of thyroid dysfunction varies considerably among patients with defective thyroglobulin synthesis, patients usually have a relatively high serum free triiodothyronine (T3) concentration with disproportionately low free tetraiodothyronine (T4) level. The maintenance of relatively high free T3 levels prevents profound tissue hypothyroidism except in brain and pituitary, which are dependent on T4 supply, resulting in neurologic and intellectual defects in some cases.
Variations in TG are associated with susceptibility to autoimmune thyroid disease type 3 (AITD3) [MIM:608175]. AITDs including Graves disease (GD) and Hashimoto thyroiditis (HT), are among the most common human autoimmune diseases. They are complex diseases, which are caused by an interaction between susceptibility genes and nongenetic factors, such as infection. -
序列相似性
Belongs to the type-B carboxylesterase/lipase family.
Contains 11 thyroglobulin type-1 domains. -
翻译后修饰
Sulfated tyrosines are desulfated during iodination. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 7038 Human
- Entrez Gene: 21819 Mouse
- Entrez Gene: 24826 Rat
- Omim: 188450 Human
- SwissProt: P01266 Human
- SwissProt: O08710 Mouse
- SwissProt: P06882 Rat
- Unigene: 654591 Human
see all -
别名
- AITD 3 antibody
- AITD3 antibody
- hTG antibody
see all
图片
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Anti-Thyroglobulin antibody [EPR9730] (ab156008) at 1/50000 dilution (purified) + Rat thyroid lysates at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 305 kDaBlocking and diluting buffer: 5% NFDM/TBST
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Intracellular Flow Cytometry analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling Thyroglobulin with purified ab156008 at 1/70 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunocytochemistry/ Immunofluorescence analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling Thyroglobulin with Purified ab156008 at 1:100 dilution. Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling Thyroglobulin with Purified ab156008 at 1:500 dilution (1.36 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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All lanes : Anti-Thyroglobulin antibody [EPR9730] (ab156008) at 1/50000 dilution (purified)
Lane 1 : Mouse thyroid lysates
Lane 2 : ICR thyroid lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Predicted band size: 305 kDaBlocking and diluting buffer: 5% NFDM/TBST
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Anti-Thyroglobulin antibody [EPR9730] (ab156008) at 1/10000 dilution (purified) + Human thyroid lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 305 kDaBlocking and diluting buffer: 5% NFDM/TBST.
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Unpurified ab156008 staining Thyroglobulin in mouse thyroid cells by intracellular flow cytometry. Cells were fixed with formaldehyde and permeabilized with permeabilization buffer. The sample was incubated with the primary antibody (1/100 in FACS buffer) for 30 minutes at 24°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Gating Strategy: Epithlial cells. Red line shows unlabeled sample, blue line shows labeled sample.
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All lanes : Anti-Thyroglobulin antibody [EPR9730] (ab156008) at 1/10000 dilution (unpurified)
Lane 1 : Mouse thyroid lysate
Lane 2 : Rat thyroid lysate
Lane 3 : Human thyroid lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 305 kDa
Observed band size: 294-300 kDa why is the actual band size different from the predicted? -
Immunohistochemical analysis of paraffin embedded Human thyroid gland follicular carcinoma tissue labeling Thyroglobulin with unpurified ab156008 antibody at 1/250.
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Immunohistochemical analysis of paraffin embedded Human thyroid gland papillary carcinoma tissue labeling Thyroglobulin with unpurified ab156008 antibody at 1/250.
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Immunofluorescent analysis of TT cells labeling Thyroglobulin with unpurified ab156008 at 1/50.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (17)
ab156008 被引用在 17 文献中.
- Zhang P et al. Targeting myeloid derived suppressor cells reverts immune suppression and sensitizes BRAF-mutant papillary thyroid cancer to MAPK inhibitors. Nat Commun 13:1588 (2022). PubMed: 35332119
- Hamada K et al. MIEAP and ATG5 are tumor suppressors in a mouse model of BRAFV600E-positive thyroid cancer. Front Endocrinol (Lausanne) 13:932754 (2022). PubMed: 36187114
- Zhang X et al. Thyroid hormone synthesis continues despite biallelic thyroglobulin mutation with cell death. JCI Insight 6:N/A (2021). PubMed: 33914707
- Wu H et al. Novel insights into di-(2-ethylhexyl)phthalate activation: Implications for the hypothalamus-pituitary-thyroid axis. Mol Med Rep 23:N/A (2021). PubMed: 33649816
- Shi L et al. The effect of the area proportion of the metastatic lesion within the central metastatic lymph node on response to therapy in papillary thyroid carcinoma. Oncol Lett 21:284 (2021). PubMed: 33732360