Anti-Thrombospondin抗体[A6.1] (ab1823)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)](https://www.abcam.cn/ps/products/1/ab1823/Images/ab1823-275744-ab1823-Supplier-IHC5.png "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)")
Key features and details
- Mouse monoclonal [A6.1] to Thrombospondin
- Suitable for: Flow Cyt, ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG1
概述
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产品名称
Anti-Thrombospondin抗体[A6.1]
参阅全部 Thrombospondin 一抗 -
描述
小鼠单克隆抗体[A6.1] to Thrombospondin -
宿主
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt RatICC/IF HumanIHC-P Human
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免疫原
BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.
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阳性对照
- Tonsil
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
纯度
IgG fraction -
克隆
单克隆 -
克隆编号
A6.1 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| 应用 | Species |
|---|---|
| Flow Cyt |
Rat
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| ICC/IF |
Human
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| IHC-P |
Human
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| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF | (4) |
Use at an assay dependent concentration.
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| IHC-P | (7) |
Use at an assay dependent concentration.
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| 说明 |
|---|
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Flow Cyt
Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. -
序列相似性
Belongs to the thrombospondin family.
Contains 3 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 1 TSP N-terminal (TSPN) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain. - Information by UniProt
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数据库链接
- Entrez Gene: 7057 Human
- Entrez Gene: 445442 Rat
- Omim: 188060 Human
- SwissProt: P07996 Human
- Unigene: 164226 Human
- Unigene: 185771 Rat
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别名
- THBS 1 antibody
- THBS antibody
- Thbs1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)
Immunohistochemical analsysis of formalin-fixed paraffin-embedded human placenta, labeling Thrombospondin with ab1823.
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![Flow Cytometry - Anti-Thrombospondin antibody [A6.1] (ab1823)](https://www.abcam.cn/ps/products/1/ab1823/Images/Thrombospondin-Primary-antibodies-ab1823-39.jpg)
Flow Cytometry - Anti-Thrombospondin antibody [A6.1] (ab1823)Overlay histogram showing PC12 cells stained with ab1823 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1823, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody [A6.1] (ab1823)](https://www.abcam.cn/ps/products/1/ab1823/Images/Thrombospondin-Primary-antibodies-ab1823-11.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody [A6.1] (ab1823)This image is courtesy of an anonymous Abreviewab1823 staining Thrombospondin in a primary culture of rat astrocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/50: 3% BSA; PBS) for 30 minutes at 20°C. A Cy3®-conjugated-Donkey polyclonal to mouse IgG (1/500) was used as secondary antibody. -
![Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody [A6.1] (ab1823)](https://www.abcam.cn/ps/products/1/ab1823/Images/Thrombospondin-Primary-antibodies-ab1823-7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody [A6.1] (ab1823)ICC/IF image of ab1823 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1823, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)](https://www.abcam.cn/ps/products/1/ab1823/Images/ab1823_2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)ab1823 at a 1/25 dilution staining human Glioblastoma Multiform tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval the tissue was stained with the antibody for 1 hour. Bound antibody was detected using biotin anti-mouse probe .
This image is courtesy of an Abreview submitted by Francisca Wu on 14 March 2006.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (52)
ab1823 被引用在 52 文献中.
- Qu S et al. MicroRNA-194 reduces inflammatory response and human dermal microvascular endothelial cells permeability through suppression of TGF-ß/SMAD pathway by inhibiting THBS1 in chronic idiopathic urticaria. J Cell Biochem 121:111-124 (2020). PubMed: 31190349
- Semba S et al. Synthetic PAMPS gel induces chondrogenic differentiation of ATDC5 cells via a novel protein reservoir function. J Biomed Mater Res A N/A:N/A (2020). PubMed: 32496623
- Wang TY et al. Maggot excretions/secretions promote diabetic wound angiogenesis via miR18a/19a - TSP-1 axis. Diabetes Res Clin Pract 165:108140 (2020). PubMed: 32277954
- Fu Y et al. Connexin 43 Modulates the Cellular Resistance to Paclitaxel via Targeting ß-Tubulin in Triple-Negative Breast Cancer. Onco Targets Ther 13:5323-5335 (2020). PubMed: 32606750
- Shih HJ et al. IGFBP3 inhibits angiogenesis through intracellular regulation of THBS1 expression. Am J Cancer Res 10:1728-1744 (2020). PubMed: 32642286