Anti-Thrombospondin抗体[A6.1] (ab1823)
Key features and details
- Mouse monoclonal [A6.1] to Thrombospondin
- Suitable for: Flow Cyt, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Sheep, Horse, Cow, Dog, Human, Pig
- Isotype: IgG1
概述
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产品名称
Anti-Thrombospondin抗体[A6.1]
参阅全部 Thrombospondin 一抗 -
描述
小鼠单克隆抗体[A6.1] to Thrombospondin -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Sheep, Horse, Cow, Dog, Human, Pig -
免疫原
BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.
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阳性对照
- Tonsil
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
纯度
IgG fraction -
克隆
单克隆 -
克隆编号
A6.1 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab1823 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ICC/IF | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. |
靶标
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功能
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. -
序列相似性
Belongs to the thrombospondin family.
Contains 3 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 1 TSP N-terminal (TSPN) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain. - Information by UniProt
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数据库链接
- Entrez Gene: 281530 Cow
- Entrez Gene: 487486 Dog
- Entrez Gene: 100057478 Horse
- Entrez Gene: 7057 Human
- Entrez Gene: 21825 Mouse
- Entrez Gene: 492313 Pig
- Entrez Gene: 445442 Rat
- Entrez Gene: 100294615 Sheep
see all -
别名
- THBS 1 antibody
- THBS antibody
- Thbs1 antibody
see all
图片
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)](https://www.abcam.cn/ps/products/1/ab1823/Images/ab1823-275744-ab1823-Supplier-IHC5.png)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)Immunohistochemical analsysis of formalin-fixed paraffin-embedded human placenta, labeling Thrombospondin with ab1823.
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![Flow Cytometry - Anti-Thrombospondin antibody [A6.1] (ab1823)](https://www.abcam.cn/ps/products/1/ab1823/Images/Thrombospondin-Primary-antibodies-ab1823-39.jpg)
Flow Cytometry - Anti-Thrombospondin antibody [A6.1] (ab1823)Overlay histogram showing PC12 cells stained with ab1823 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab1823, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. -
![Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody [A6.1] (ab1823)](https://www.abcam.cn/ps/products/1/ab1823/Images/Thrombospondin-Primary-antibodies-ab1823-11.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody [A6.1] (ab1823)This image is courtesy of an anonymous Abreviewab1823 staining Thrombospondin in a primary culture of rat astrocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton and blocked with 3% BSA for 15 minutes at 20°C. Samples were incubated with primary antibody (1/50: 3% BSA; PBS) for 30 minutes at 20°C. A Cy3®-conjugated-Donkey polyclonal to mouse IgG (1/500) was used as secondary antibody. -
![Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody [A6.1] (ab1823)](https://www.abcam.cn/ps/products/1/ab1823/Images/Thrombospondin-Primary-antibodies-ab1823-7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin antibody [A6.1] (ab1823)ICC/IF image of ab1823 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1823, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)](https://www.abcam.cn/ps/products/1/ab1823/Images/ab1823_2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin antibody [A6.1] (ab1823)ab1823 at a 1/25 dilution staining human Glioblastoma Multiform tissue sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval the tissue was stained with the antibody for 1 hour. Bound antibody was detected using biotin anti-mouse probe .
This image is courtesy of an Abreview submitted by Francisca Wu on 14 March 2006.
实验方案
文献 (52)
ab1823 被引用在 52 文献中.
- Qu S et al. MicroRNA-194 reduces inflammatory response and human dermal microvascular endothelial cells permeability through suppression of TGF-ß/SMAD pathway by inhibiting THBS1 in chronic idiopathic urticaria. J Cell Biochem 121:111-124 (2020). PubMed: 31190349
- Semba S et al. Synthetic PAMPS gel induces chondrogenic differentiation of ATDC5 cells via a novel protein reservoir function. J Biomed Mater Res A N/A:N/A (2020). PubMed: 32496623
- Wang TY et al. Maggot excretions/secretions promote diabetic wound angiogenesis via miR18a/19a - TSP-1 axis. Diabetes Res Clin Pract 165:108140 (2020). PubMed: 32277954
- Fu Y et al. Connexin 43 Modulates the Cellular Resistance to Paclitaxel via Targeting ß-Tubulin in Triple-Negative Breast Cancer. Onco Targets Ther 13:5323-5335 (2020). PubMed: 32606750
- Shih HJ et al. IGFBP3 inhibits angiogenesis through intracellular regulation of THBS1 expression. Am J Cancer Res 10:1728-1744 (2020). PubMed: 32642286
