重组Anti-Thrombospondin 1抗体[EPR22928-10] (ab263905)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22928-10] to Thrombospondin 1
- Suitable for: WB, IHC-Fr, IP, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-Thrombospondin 1抗体[EPR22928-10]
参阅全部 Thrombospondin 1 一抗 -
描述
兔单克隆抗体[EPR22928-10] to Thrombospondin 1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Fr, IP, IHC-Pmore details
不适用于: Flow Cyt (Intra) or ICC/IF -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: 3T3-L1 starved with 0.4% serum for 24 hours, then cultivated with 15% serum for 6 hours, whole cell lysate, Rat and mouse platelet whole cell lysate. IHC-P: Human spleen and bone marrow, Mouse and rat spleen tissue. IHC-Fr: Mouse and rat spleen tissue. IP: Mouse platelet cell lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR22928-10 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab263905于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 129 kDa).
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IHC-Fr |
1/500.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
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IP |
1/30.
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IHC-P |
1/2000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Use at 1/5000 for human, 1/2000 for mouse and rat dilution. |
说明 |
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WB
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 129 kDa). |
IHC-Fr
1/500. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
IP
1/30. |
IHC-P
1/2000 - 1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Use at 1/5000 for human, 1/2000 for mouse and rat dilution. |
靶标
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功能
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Binds heparin. May play a role in dentinogenesis and/or maintenance of dentin and dental pulp (By similarity). Ligand for CD36 mediating antiangiogenic properties. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors. -
序列相似性
Belongs to the thrombospondin family.
Contains 2 EGF-like domains.
Contains 1 laminin G-like domain.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 3 TSP type-1 domains.
Contains 8 TSP type-3 repeats.
Contains 1 VWFC domain. -
细胞定位
Endoplasmic reticulum. Sarcoplasmic reticulum. - Information by UniProt
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数据库链接
- Entrez Gene: 7057 Human
- Entrez Gene: 21825 Mouse
- Entrez Gene: 445442 Rat
- Omim: 188060 Human
- SwissProt: P07996 Human
- SwissProt: P35441 Mouse
- SwissProt: Q8CGB2 Mouse
- Unigene: 164226 Human
see all -
别名
- Thbs1 antibody
- Thrombospondin-1 antibody
- TSP antibody
see all
图片
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat spleen tissue labeling Thrombospondin 1 with ab263905 at 1/500 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining on rat splenic megakaryocytes and platelet (PMID: 28239144) is observed. Nuclear counterstain DAPI.
Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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All lanes : Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905) at 1/1000 dilution
Lane 1 : Mouse platelet whole cell lysate
Lane 2 : Rat platelet whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 129 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBSTExposure time: Lane 1: 59 seconds Lane 2: 15 seconds
The identity of the lower MW bands at approximately 25 kDa is unknown.
The bands beneath the target band (~75-180 kDa) are likely to be degraded target fragments. -
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling Thrombospondin 1 with ab263905 at 1/2000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the megakaryocytes and platelets in the rat spleen. The section was incubated with ab263905 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Thrombospondin 1 was immunoprecipitated from 0.35 mg mouse platelet lysate 10µg with ab263905 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263905 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: Mouse platelet lysate 10µg
Lane 2: ab263905 IP in mouse platelet lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263905 in mouse platelet lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 mins.
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse spleen tissue labeling Thrombospondin 1 with ab263905 at 1/500 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution. Positive staining on mouse splenic megakaryocytes and platelet (PMID: 28239144) is observed. Nuclear counterstain DAPI.
Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Thrombospondin 1 with ab263905 at 1/2000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the megakaryocytes and platelets in the mouse spleen. The section was incubated with ab263905 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Thrombospondin 1 with ab263905 at 1/5000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the platelets in the human spleen (PMID: 28239144). The section was incubated with ab263905 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded human bone marrow tissue labeling Thrombospondin 1 with ab263905 at 1/5000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the megakaryocytes in the human bone marrow (PMID: 28239144). The section was incubated with ab263905 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905) at 1/1000 dilution + Mouse platelet lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/25000 dilution
Predicted band size: 129 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 27588705).
Exposure time: 1 second.
Observed MW: 180 kDa.
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All lanes : Anti-Thrombospondin 1 antibody [EPR22928-10] (ab263905) at 1/1000 dilution
Lane 1 : 3T3-L1 (mouse embryonic fibroblast) starved with 0.4% serum for 30 hours whole cell lysate
Lane 2 : 3T3-L1 starved with 0.4% serum for 24 hours, then cultivated with 15% serum for 6 hours. whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/25000 dilution
Predicted band size: 129 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
TSP 1 is a serum-responsive gene. Its express is elevated in quiescent 3T3 with serum addition.
The molecular weight observed is consistent with what has been described in the literature (PMID: 8422970, 27588705).
Exposure time: 3 minutes.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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