Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (4-20% mini gel)
Sample
Mouse Tissue lysate - whole (Penile Tissue)
Specification
Penile Tissue
Blocking step
SuperBlock Blocking Buffer (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: SuperBlock Blocking Buffer (PBS)
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: ECL Anti-mouse IgG
Host species: Sheep
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
Chemiluminescence, ECL
Bands
Specific: ~170 kDa
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Negative control
I did not have a negative control
Positive control
I did not have a positive control
Additional data
Additional Notes
I think this antibody works well but needs to be tweaked a bit. The background seems high and I would probably use a more concentrated primary and less concentrated secondary the next time.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Miss. Gwen Lagoda
Verified customer
提交于 Nov 11 2014