重组Anti-Thrombomodulin抗体[EPR18217-209] (ab230010)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18217-209] to Thrombomodulin
- Suitable for: IHC-Fr, ICC/IF, IP, Flow Cyt, IHC-P, WB
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-Thrombomodulin抗体[EPR18217-209]
参阅全部 Thrombomodulin 一抗 -
描述
兔单克隆抗体[EPR18217-209] to Thrombomodulin -
宿主
Rabbit -
经测试应用
适用于: IHC-Fr, ICC/IF, IP, Flow Cyt, IHC-P, WBmore details -
种属反应性
与反应: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Mouse lung and placenta tissue lysates; bEND.3 whole cell lysate. IHC-P: Mouse lung and stomach tissues. IHC-Fr: Mouse embryo E14.5 (developing lung) tissue. ICC/IF: bEND.3 cells. Flow Cyt: bEND.3 cells. IP: bEND.3 whole cell lysate; Mouse lung tissue lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR18217-209 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab230010于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
1/500.
Perform heat mediated antigen retrieval by using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20). |
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ICC/IF |
1/100.
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IP |
1/30.
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Flow Cyt |
1/500.
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Detects a band of approximately 75,105 kDa (predicted molecular weight: 62 kDa).
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说明 |
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IHC-Fr
1/500. Perform heat mediated antigen retrieval by using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20). |
ICC/IF
1/100. |
IP
1/30. |
Flow Cyt
1/500. |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 75,105 kDa (predicted molecular weight: 62 kDa). |
靶标
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功能
Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated. -
组织特异性
Endothelial cells are unique in synthesizing thrombomodulin. -
疾病相关
Defects in THBD are the cause of thrombophilia due to thrombomodulin defect (THR-THBD) [MIM:188040]. A hemostatic disorder characterized by a tendency to thrombosis.
Defects in THBD are a cause of susceptibility to hemolytic uremic syndrome atypical type 6 (AHUS6) [MIM:612926]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. -
序列相似性
Contains 1 C-type lectin domain.
Contains 6 EGF-like domains. -
翻译后修饰
N-glycosylated.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 21824 Mouse
- SwissProt: P15306 Mouse
- Unigene: 24096 Mouse
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别名
- AHUS 6 antibody
- AHUS6 antibody
- BDCA 3 antibody
see all
图片
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All lanes : Anti-Thrombomodulin antibody [EPR18217-209] (ab230010) at 1/1000 dilution
Lane 1 : Mouse lung tissue lysate at 20 µg
Lane 2 : bEND.3 (mouse brain endothelioma cell line) whole cell lysate at 10 µg
Lane 3 : Mouse placenta tissue lysate at 10 µg
Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 2-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 62 kDa
Observed band size: 105,75 kDa why is the actual band size different from the predicted?Exposure times: Lane 1: 1 minute; Lane 2: 20 seconds; Lane 3: 10 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized bEND.3 (mouse brain endothelioma cell line) cells labeling Thrombomodulin with ab230010 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in bEND.3 cell line (PMID: 7622601; PMID: 8223719).
The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution. -
Thrombomodulin was immunoprecipitated from 0.35 mg of mouse lung tissue lysate with ab230010 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230010 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: Mouse lung tissue lysate 10 μg (Input).
Lane 2: ab230010 IP in mouse lung tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230010 in mouse lung tissue lysate.Exposure time: 10 seconds.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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Thrombomodulin was immunoprecipitated from 0.35 mg of bEND.3 (mouse brain endothelioma cell line) whole cell lysate with ab230010 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230010 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: bEND.3 whole cell lysate 10 μg (Input).
Lane 2: ab230010 IP in bEND.3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab230010 in bEND.3 whole cell lysate.Exposure time: 10 seconds.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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Flow cytometric analysis of bEND.3 (mouse brain endothelioma cell line) cells labeling Thrombomodulin with ab230010 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
Gated on total viable cells.
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse embryo E14.5 (developing lung) tissue labeling Thrombomodulin with ab230010 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green). Positive membrane staining in the developing lung in mouse E14.5 embryo (PMID: 28306049) is observed.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Thrombomodulin with ab230010 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on endothelial cells of mouse lung (PMID: 23946288; PMID: 10231031) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Thrombomodulin with ab230010 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on endothelial cells of mouse stomach (PMID: 23946288; PMID: 10231031) is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (2)
ab230010 被引用在 2 文献中.
- Kondo T et al. Immunohistochemical analysis of thrombomodulin expression in myocardial tissue from autopsy cases of ischemic heart disease. Leg Med (Tokyo) 51:101897 (2021). PubMed: 33940278
- Dobie R et al. Single-Cell Transcriptomics Uncovers Zonation of Function in the Mesenchyme during Liver Fibrosis. Cell Rep 29:1832-1847.e8 (2019). PubMed: 31722201