参阅全部 Thrombin 一抗
经测试应用适用于: WBmore details
种属反应性与反应: Mouse, Rat, Human
预测可用于: Sheep, Rabbit, Pig, Macaque monkey, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human Thrombin.
- This antibody gave a positive signal in the following lysates: Human Liver Tissue; Mouse Liver Tissue; Rat Liver Tissue; Human Plasma Total Protein; Mouse Plasma
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab92621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 70 kDa).|
功能Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.
组织特异性Expressed by the liver and secreted in plasma.
疾病相关Factor II deficiency
Thrombophilia due to thrombin defect
Pregnancy loss, recurrent, 2
序列相似性Belongs to the peptidase S1 family.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 kringle domains.
Contains 1 peptidase S1 domain.
翻译后修饰The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.
N-glycosylated. N-glycan heterogeneity at Asn-121: Hex3HexNAc3 (minor), Hex4HexNAc3 (minor) and Hex5HexNAc4 (major). At Asn-143: Hex4HexNAc3 (minor) and Hex5HexNAc4 (major).
细胞定位Secreted, extracellular space.
- Information by UniProt
- Coagulation factor II antibody
- Coagulation factor II thrombin antibody
- EC 220.127.116.11 antibody
All lanes : Anti-Thrombin antibody (ab92621) at 1 µg/ml
Lane 1 : Liver (Human) Tissue Lysate
Lane 2 : Liver (Mouse) Tissue Lysate
Lane 3 : Liver (Rat) Tissue Lysate
Lane 4 : Plasma (Human) Total Protein Lysate
Lane 5 : Plasma (Mouse) Total Protein Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab92621 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
This product has been referenced in:
- Kobori T et al. Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis. Front Immunol 9:334 (2018). Read more (PubMed: 29559970) »
- Man-Kupisinska A et al. A New Ligand-Based Method for Purifying Active Human Plasma-Derived Ficolin-3 Complexes Supports the Phenomenon of Crosstalk between Pattern-Recognition Molecules and Immunoglobulins. PLoS One 11:e0156691 (2016). Read more (PubMed: 27232184) »