Application
Western blot
Sample
Rat Cell lysate - whole cell (MCF-7, Rat brain E19, Cortical neurons DIV6/12)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
40 µg
Specification
MCF-7, Rat brain E19, Cortical neurons DIV6/12
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: RABBIT IgG (H&L) Antibody IRDye800
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Odyssey Imager
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 65-70 kDa Non-specific: 120-125, >150, <25 kDa
Positive control
MCF-7
Additional data
Additional Notes
Lysis buffer used: RIPA except where mentioned. WB membrane - Odyssey Nitrocellulose Membrane
Lane 1: Ladder (Kaleidoscope BIO-RAD)
Lane 2: MCF-7 (Lysis buffer - 1% SDS)
Lane 3: MCF-7
Lane 4: Rat Brain E19
Lane 5: Primary rat cortical neurons day in vitro 6
Lane 6: Primary rat cortical neurons day in vitro 12
Odyssey - Intensity 8.0
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提交于 Jun 15 2016