使用敲除细胞株进行验证重组RabMAb

重组Anti-TGF beta 1抗体[EPR21143] (ab215715)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21143] to TGF beta 1
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

概述

  • 产品名称

    Anti-TGF beta 1抗体[EPR21143]
    参阅全部 TGF beta 1 一抗

  • 描述

    兔单克隆抗体[EPR21143] to TGF beta 1

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment within Mouse TGF beta 1 aa 150-400. The exact sequence is proprietary.
    Database link: P04202

  • 阳性对照

    • WB: NIH/3T3, L-929, HeLa, A549, HL-60 and RAW 264.7 whole cell lysates; Mouse spleen lysate. IHC-P: Human thrombocytosis tissue; Mouse and rat spleen tissues.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

应用

Our Abpromise guarantee covers the use of ab215715 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 44 kDa.

Actual band: 13 kDa and 44 kDa
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

靶标

  • 功能

    Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.

  • 组织特异性

    Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.

  • 疾病相关

    Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.

  • 序列相似性

    Belongs to the TGF-beta family.

  • 翻译后修饰

    Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.

  • 细胞定位

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Cartilage-inducing factor antibody
    • CED antibody
    • Differentiation inhibiting factor antibody
    • DPD1 antibody
    • LAP antibody
    • Latency-associated peptide antibody
    • Prepro transforming growth factor beta 1 antibody
    • TGF beta 1 antibody
    • TGF beta antibody
    • TGF beta 1 protein antibody
    • TGF-beta 1 protein antibody
    • TGF-beta-1 antibody
    • TGF-beta-5 antibody
    • TGF-beta1 antibody
    • TGFB antibody
    • Tgfb-1 antibody
    • tgfb1 antibody
    • TGFB1_HUMAN antibody
    • TGFbeta antibody
    • TGFbeta1 antibody
    • Transforming Growth Factor b1 antibody
    • Transforming Growth Factor beta 1 antibody
    • Transforming growth factor beta 1a antibody
    • transforming growth factor beta-1 antibody
    • transforming growth factor, beta 1 antibody
    • Transforming Growth Factor-ß1 antibody
    see all

图片

  • Western blot - Anti-TGF beta 1 antibody [EPR21143] (ab215715)
    Western blot - Anti-TGF beta 1 antibody [EPR21143] (ab215715)
    All lanes : Anti-TGF beta 1 antibody [EPR21143] (ab215715) at 1/1000 dilution

    Lane 1 : Wild-type HeLa whole cell lysate
    Lane 2 : TGFB1 knockout HeLa whole cell lysate
    Lane 3 : A549 whole cell lysate
    Lane 4 : Mouse spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 44 kDa
    Observed band size: 13,44 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab215715 observed at 13 and 44 kDa. Red - loading control, ab7291, observed at 50 kDa.

    ab215715 was shown to specifically react with in wild-type HeLa cells as signal was lost in TGFB1 knockout cells. Wild-type and TGFB1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab215715 and ab7291 (Mouse anti-tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta 1 antibody [EPR21143] (ab215715)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta 1 antibody [EPR21143] (ab215715)

    Immunohistochemical analysis of paraffin-embedded human thrombocytosis tissue labeling TGF beta 1 with ab215715 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining of megakaryocytes in human thrombocytosis (PMID: 25305163). Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta 1 antibody [EPR21143] (ab215715)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta 1 antibody [EPR21143] (ab215715)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling TGF beta 1 with ab215715 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining of megakaryocytes and platelets in rat spleen (PMID: 25305163). Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta 1 antibody [EPR21143] (ab215715)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGF beta 1 antibody [EPR21143] (ab215715)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling TGF beta 1 with ab215715 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining of megakaryocytes and platelets in mouse spleen (PMID: 25305163). Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

  • Western blot - Anti-TGF beta 1 antibody [EPR21143] (ab215715)
    Western blot - Anti-TGF beta 1 antibody [EPR21143] (ab215715)
    All lanes : Anti-TGF beta 1 antibody [EPR21143] (ab215715) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg
    Lane 2 : L-929 (mouse connective tissue fibroblast cell line) whole cell lysate at 20 µg
    Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate at 20 µg
    Lane 4 : HL-60 (human promyelocytic leukemia cell line) whole cell lysate at 20 µg
    Lane 5 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 20 µg
    Lane 6 : Mouse spleen lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 44 kDa
    Observed band size: 12,44 kDa why is the actual band size different from the predicted?



     

    Exposure times: Lanes 1-4: 3 minutes; Lane 5: 41 seconds; Lane 6: 24 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The molecular mass observed is consistent with the literature (PMID 2139036).

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

     

  • Anti-TGF beta 1 antibody [EPR21143] (ab215715)
    Anti-TGF beta 1 antibody [EPR21143] (ab215715)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (6)

发表研究结果有使用 ab215715？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab215715 被引用在 6 文献中.

  • Wang J  et al. Microcystin-LR ameliorates pulmonary fibrosis via modulating CD206+ M2-like macrophage polarization. Cell Death Dis 11:136 (2020). PubMed: 32075954
  • Huo Q  et al. Acetazolamide attenuates cardiac fibrosis induced by aortic constriction through inhibiting transforming growth factor-ß1/Smad2 signaling pathway in mice. Exp Ther Med 17:2317-2321 (2019). PubMed: 30867716
  • Dombrowsky AC  et al. Acute Skin Damage and Late Radiation-Induced Fibrosis and Inflammation in Murine Ears after High-Dose Irradiation. Cancers (Basel) 11:N/A (2019). PubMed: 31130616
  • Kang SH  et al. Effects of tranilast on the epithelial-to-mesenchymal transition in peritoneal mesothelial cells. Kidney Res Clin Pract 38:472-480 (2019). PubMed: 31554027
  • Xing L  et al. Nephroprotective effect of losartan in IgA model rat. J Int Med Res 47:5205-5215 (2019). PubMed: 31638466
  • An H  et al. Stomatin plays a suppressor role in non-small cell lung cancer metastasis. Chin J Cancer Res 31:930-944 (2019). PubMed: 31949395

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TGF beta 1 antibody [EPR21143]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH9
Permeabilization
No
Specification
Spleen
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
Formaldehyde
Read More

Mr. Victor Bernal

Verified customer

提交于 Oct 26 2020

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