Key features and details
- Rabbit polyclonal to TFIIB (acetyl K238)
- Suitable for: ELISA, ICC/IF
- Isotype: IgG
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
纯度Immunogen affinity purified
Immunizing Peptide (Blocking)
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 5 µg/ml.|
功能General factor that plays a major role in the activation of eukaryotic genes transcribed by RNA polymerase II.
序列相似性Belongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger.
- Information by UniProt
- General transcription factor IIB antibody
- General transcription factor TFIIB antibody
- gtf2b antibody
This antibody gave a positive result in ELISA against the immunising peptide (ab26407) and a negative result using the non-modified equivalent peptide (ab25428). This indicates that it is specific for the modified peptide.
ICC/IF image of ab5210 stained HeLa cells. The cells were 100% methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab5210, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 4% PFA and 100% methanol fixed Hek293, HepG2 and Mcf7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab5210 被引用在 1 文献中.
- Gelev V et al. A new paradigm for transcription factor TFIIB functionality. Sci Rep 4:3664 (2014). WB ; Human . PubMed: 24441171