Anti-TFEB抗体(ab2636)
Key features and details
- Goat polyclonal to TFEB
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
重组抗体的批间可重复性更强
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-TFEB抗体
参阅全部 TFEB 一抗 -
描述
山羊多克隆抗体to TFEB -
宿主
Goat -
特异性
This antibody is raised against TCFEB. However a customer reports that this antibody cross reacts with mouse TFE3 and MITF (other members of transcription factor family Mi/Tfe) in over-expressing cell lines. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow, Dog, Pig, Chinese hamster -
免疫原
Synthetic peptide corresponding to Human TFEB aa 2-14 (N terminal).
Sequence:ASRIGLRMQLMRE
Database link: P19484 -
阳性对照
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常规说明
Based on sequence homology, TFEB isoforms 1 and 2 (NP_009093.1 and NP_001161299.2) with MW of 52.9 and 54.2 kDa respectively can be detected by this antibody
Principal Names - TFEB; transcription factor EB; TCFEB; T-cell transcription factor EB Official Gene Symbol - TFEB GenBank Accession Number – NP_009093 LocusLink ID - 7942 (human); 21425 (mouse)
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab2636 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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WB | Use a concentration of 0.3 - 1 µg/ml. Primary incubation 1 hour at room temperature. Blocking in milk. Detects band(s) of approximately 53 kDa. Predicted molecular weights of TFEB isoforms 1 and 2 are 52.9 and 54.2 kDa respectively.
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靶标
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功能
Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta. -
序列相似性
Belongs to the MiT/TFE family.
Contains 1 basic helix-loop-helix (bHLH) domain. -
结构域
The leucin zipper region is essential for homo- or heterodimerization and high-affinity DNA binding. DNA binding is mediated by the basic region. -
翻译后修饰
Sumoylated; does not affect dimerization with MITF. -
细胞定位
Cytoplasm. Nucleus. Mainly present in the cytoplasm. Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 510032 Cow
- Entrez Gene: 609046 Dog
- Entrez Gene: 7942 Human
- Entrez Gene: 21425 Mouse
- Entrez Gene: 100157940 Pig
- Entrez Gene: 316214 Rat
- Omim: 600744 Human
- SwissProt: P19484 Human
see all -
别名
- Alpha TFEB antibody
- AlphaTFEB antibody
- bHLHe35 antibody
see all
图片
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Lane 1 : Anti-TFEB antibody (ab2636) at 0.5 µg/ml
Lane 2 : Anti-TFEB antibody (ab2636) at 0.3 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysatePrimary incubation was 1 hour. Detected by chemiluminescence.
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Anti-TFEB antibody (ab2636) at 1 µg/ml + Human thymus lysate in RIPA buffer at 35 µg
Observed band size: 60 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
数据表及文件
文献 (35)
ab2636 被引用在 35 文献中.
- Kim M et al. Comprehensive Immunoprofiles of Renal Cell Carcinoma Subtypes. Cancers (Basel) 12:N/A (2020). PubMed: 32150988
- Zhou J et al. Thyroid Hormone Status Regulates Skeletal Muscle Response to Chronic Motor Nerve Stimulation. Front Physiol 10:1363 (2019). PubMed: 31736784
- Kojima F et al. Aggressive TFEB-rearranged renal cell carcinoma mimicking chromophobe and clear cell renal cell carcinoma. Pathol Int 69:51-53 (2019). PubMed: 30620430
- Liu N et al. Lactate inhibits ATP6V0d2 expression in tumor-associated macrophages to promote HIF-2a-mediated tumor progression. J Clin Invest N/A:N/A (2019). PubMed: 30431439
- Magini A et al. Curcumin Analogue C1 Promotes Hex and Gal Recruitment to the Plasma Membrane via mTORC1-Independent TFEB Activation. Int J Mol Sci 20:N/A (2019). PubMed: 30889901
客户评价及客户问答
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