Anti-TFEB抗体(ab2636)

Reviews (4)Q&A (1)References (35)
Western blot - Anti-TFEB antibody (ab2636)
  • Western blot - Anti-TFEB antibody (ab2636)

Key features and details

  • Goat polyclonal to TFEB
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

重组抗体的批间可重复性更强

Product image
Anti-TFEB antibody [EPR22940-151] (ab270604)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术，可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-TFEB抗体
    参阅全部 TFEB 一抗

  • 描述

    山羊多克隆抗体to TFEB

  • 宿主

    Goat

  • 特异性

    This antibody is raised against TCFEB. However a customer reports that this antibody cross reacts with mouse TFE3 and MITF (other members of transcription factor family Mi/Tfe) in over-expressing cell lines.

  • 经测试应用

    适用于: WBmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Pig, Chinese hamster

  • 免疫原

    Synthetic peptide corresponding to Human TFEB aa 2-14 (N terminal).
    Sequence:

    ASRIGLRMQLMRE


    Database link: P19484
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • 阳性对照

  • 常规说明

    Based on sequence homology, TFEB isoforms 1 and 2 (NP_009093.1 and NP_001161299.2) with MW of 52.9 and 54.2 kDa respectively can be detected by this antibody

    Principal Names - TFEB; transcription factor EB; TCFEB; T-cell transcription factor EB Official Gene Symbol - TFEB GenBank Accession Number – NP_009093 LocusLink ID - 7942 (human); 21425 (mouse)  

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

应用

Our Abpromise guarantee covers the use of ab2636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml.

Primary incubation 1 hour at room temperature. Blocking in milk. 

Detects band(s) of approximately 53 kDa. Predicted molecular weights of TFEB isoforms 1 and 2 are 52.9 and 54.2 kDa respectively. 

 

靶标

  • 功能

    Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta.

  • 序列相似性

    Belongs to the MiT/TFE family.
    Contains 1 basic helix-loop-helix (bHLH) domain.

  • 结构域

    The leucin zipper region is essential for homo- or heterodimerization and high-affinity DNA binding. DNA binding is mediated by the basic region.

  • 翻译后修饰

    Sumoylated; does not affect dimerization with MITF.

  • 细胞定位

    Cytoplasm. Nucleus. Mainly present in the cytoplasm. Under aberrant lysosomal storage conditions, it translocates from the cytoplasm to the nucleus.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Alpha TFEB antibody
    • AlphaTFEB antibody
    • bHLHe35 antibody
    • Class E basic helix-loop-helix protein 35 antibody
    • T cell transcription factor EB antibody
    • TCFEB antibody
    • TFEB antibody
    • TFEB_HUMAN antibody
    • Transcription factor EB antibody
    see all

图片

  • Western blot - Anti-TFEB antibody (ab2636)
    Western blot - Anti-TFEB antibody (ab2636)
    Lane 1 : Anti-TFEB antibody (ab2636) at 0.5 µg/ml
    Lane 2 : Anti-TFEB antibody (ab2636) at 0.3 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate


    Primary incubation was 1 hour. Detected by chemiluminescence.

  • Western blot - Anti-TFEB antibody (ab2636)
    Western blot - Anti-TFEB antibody (ab2636)
    Anti-TFEB antibody (ab2636) at 1 µg/ml + Human thymus lysate in RIPA buffer at 35 µg

    Observed band size: 60 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.

文献 (35)

发表研究结果有使用 ab2636？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab2636 被引用在 35 文献中.

  • Kim M  et al. Comprehensive Immunoprofiles of Renal Cell Carcinoma Subtypes. Cancers (Basel) 12:N/A (2020). PubMed: 32150988
  • Zhou J  et al. Thyroid Hormone Status Regulates Skeletal Muscle Response to Chronic Motor Nerve Stimulation. Front Physiol 10:1363 (2019). PubMed: 31736784
  • Kojima F  et al. Aggressive TFEB-rearranged renal cell carcinoma mimicking chromophobe and clear cell renal cell carcinoma. Pathol Int 69:51-53 (2019). PubMed: 30620430
  • Liu N  et al. Lactate inhibits ATP6V0d2 expression in tumor-associated macrophages to promote HIF-2a-mediated tumor progression. J Clin Invest N/A:N/A (2019). PubMed: 30431439
  • Magini A  et al. Curcumin Analogue C1 Promotes Hex and Gal Recruitment to the Plasma Membrane via mTORC1-Independent TFEB Activation. Int J Mol Sci 20:N/A (2019). PubMed: 30889901
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-TFEB antibody - ChIP Grade

 Good
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - nuclear (MEFs)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
MEFs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C
Read More

Abcam user community

Verified customer

提交于 Jul 04 2018

Western blot abreview for Anti-TFEB antibody - ChIP Grade

 Average
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (NuPAGE® Novex® 10% gel)
Loading amount
30 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Dr. Insu Kwon

Verified customer

提交于 Dec 10 2015

Western blot abreview for Anti-TFEB antibody - ChIP Grade

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
35 µg
Specification
Brain
Blocking step
Li-Cor Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
Read More

Abcam user community

Verified customer

提交于 Jan 22 2015

Question

Hello, I recently purchased Anti-TFEB antibody - ChIP Grade (ab2636). Since it says that it is "ChIP grade,' my lab is planning on doing a few experiments involving ChIP, but before we do so we were wondering what genes/promotors abcam has used to verify that this antibody is suitable for ChIP? As much detailed information including controls, cell/tissue types probed, reaction conditions and buffers, etc. would be extremely helpful.

Read More
Answer

Thank you for your inquiry.

We do not batch test this antibody for ChIP in-house, but this antibody has been successfully used in ChIP as described in the following papers:

Esumi N et al. VMD2 promoter requires two proximal E-box sites for its activity in vivo and is regulated by the MITF-TFE family. J Biol Chem 282:1838-50 (2007). PMID: 17085443.

Huan C et al. Renal Carcinoma-associated Transcription Factors TFE3 and TFEB Are Leukemia Inhibitory Factor-responsive Transcription Activators of E-cadherin. J Biol Chem 280:30225-35 (2005). PubMed: 15994295

We would guarantee this antibody to work in ChIP in accordance with our Abpromise.

I hope this information helps. Please contact us with any other questions.

Read More

Western blot abreview for Anti-TFEB antibody - ChIP Grade

 Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - nuclear (breast cancer cell lines)
Loading amount
40 µg
Specification
breast cancer cell lines
Gel Running Conditions
Reduced Denaturing (8 % acrylamide gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Sep 01 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com