Texas Red ® Anti-Biotin抗体(ab6653)

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Western blot - Texas Red ® Anti-Biotin antibody (ab6653)

    Key features and details

    • Texas Red ® Goat polyclonal to Biotin
    • Suitable for: WB
    • Reacts with: Species independent
    • Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
    • Isotype: IgG

    概述

    • 产品名称

      Texas Red ® Anti-Biotin抗体
      参阅全部 Biotin 一抗

    • 描述

      Texas Red ®山羊多克隆抗体to生物素

    • 宿主

      Goat

    • 偶联物

      Texas Red ®. Ex: 596nm, Em: 620nm

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Species independent

    • 免疫原

      Chemical/ Small Molecule conjugated to KLH.

    • 常规说明

      Texas Red™ is a registered trademark of Molecular Probes Inc.

      Texas Red™ Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.5 moles Texas Red™ per mole of Goat IgG.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6653于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use at an assay dependent concentration.
    说明
    WB
    Use at an assay dependent concentration.

    靶标

    • 相关性

      Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.

    • 别名

      • Vitamin B4 antibody

    图片

    • Western blot - Texas Red ® Anti-Biotin antibody (ab6653)
      Western blot - Texas Red ® Anti-Biotin antibody (ab6653)
      Texas Red ® Anti-Biotin antibody (ab6653) at 1/1000 dilution (60 minutes at room temperature) + Biotin conjugated Guinea Pig IgG separated by SDS-PAGE at 0.05 µg

      Observed band size: 25,55 kDa why is the actual band size different from the predicted?



      Blocking Buffer for Fluorescent Western Blotting for 30 min at room temperature.

    文献 (0)

    发表研究结果有使用 ab6653？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab6653 尚未被引用在任何文献中。

    客户评价及客户问答

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    Question

    Our customer would like to know specifically what you used for the secondary as this primary is conjugated with Texas Red. Thanks again!

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    Answer

    There are still free epitopes available for a secondary antibody to bind. But you could also use an unconjugated secondary antibody.

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    Question

    Hi Jennifer Nice to talk with you. Please see the below answers attached to each questions what you had. Please reply ASAP.Thanks! Have a nice day! technical@abcam.com wrote: Dear Wenxin, I have some general questions, the answers to which will enable me to investigate this matter as quickly as possible. 1. Please describe the problem (high background, no staining etc). The particulate precipitate was observed from antibody-solution in the vial and also was found in the nodules section under confocal microscope. 2. On what material are you testing the antibody in IHC? •Species? Soybean (Glycine max) •Cell line? •Tissue? Nodules 3. How did you fix the samples? •Ethanol, methanol •Acetone •Paraformaldehyde and Other FAA 4. Did you apply antigen retrieval step? No •Enzymatic method •Heat mediated technique •Other 5. How did you block the unspecific binding sites? 3% BSA in TBST buffer 6. Primary antibody •Specification (in which species was it raised against)? Products was labeled with Biotin •At what dilution(s) have you tested this antibody? 1: 100 •Incubation time, wash steps (multiple short washes are more effective than fewer longer wash steps)? 1-2 hr at RT; 2 x 5 min with TBST buffer 7. Secondary antibody NO •What secondary antibody are you using? •Specification (in which species was it raised against)? •At what dilution(s) have you tested this antibody? •Incubation, wash steps? •Do you know whether the problems you are experiencing come from the secondary? •What detection method are you using? 8. Background staining •Please provide an image of your staining we have no result for this experiment for several times but we have very nice data when we use Dig-system. 9. Which detection system did you use? confocal microscope 10. Did you apply positive and negative controls along with the samples? Please specify. Yes, no products were generated under negative control. For positive, we used biotin-anti--Dig. 11. Optimization attempts •How many times have you tried the IHC? Three times for each fixed sample (FAA or Paraformaldehyde) •Do you obtain the same results every time? Yes •What steps have you altered? No

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    Answer

    Thank you very much for the information that you have provided. You should not have precipitate in your vial and so I'm sending you a replacement vial free of charge. For your record it is order# 39078 and you should receive it Tuesday or Wednesday of next week. Please let me know how the replacement works out for you.

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    Question

    Hello, Can you tell me how many dyes (texas Red) are attached per molecule of anti-biotin antibody? Waiting for your reply and thanking you in advance. Cheers.

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    Answer

    The molar ratios of dye-to-protein are varied batch to batch. The general acceptance criteria for this product is 2-5:1

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