Key features and details
- Texas Red ® Goat polyclonal to Biotin
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: Texas Red ®. Ex: 596nm, Em: 620nm
- Isotype: IgG
产品名称Texas Red ® Anti-Biotin抗体
参阅全部 Biotin 一抗
描述Texas Red ®山羊多克隆抗体to生物素
偶联物Texas Red ®. Ex: 596nm, Em: 620nm
经测试应用适用于: WBmore details
种属反应性与反应: Species independent
Chemical/ Small Molecule conjugated to KLH.
Texas Red™ is a registered trademark of Molecular Probes Inc.
Texas Red™ Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 2.5 moles Texas Red™ per mole of Goat IgG.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
Concentration information loading...
纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to sepharose beads.
Our Abpromise guarantee covers the use of ab6653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.|
相关性Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.
- Vitamin B4 antibody
Texas Red ® Anti-Biotin antibody (ab6653) at 1/1000 dilution (60 minutes at room temperature) + Biotin conjugated Guinea Pig IgG separated by SDS-PAGE at 0.05 µg
Observed band size: 25,55 kDa why is the actual band size different from the predicted?
Blocking Buffer for Fluorescent Western Blotting for 30 min at room temperature.