Key features and details
- Rabbit polyclonal to Tex19.1
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
经测试应用适用于: WBmore details
Synthetic peptide corresponding to Mouse Tex19.1 aa 300 to the C-terminus conjugated to keyhole limpet haemocyanin.
Database link: Q99MV2
- This antibody gave a positive signal in both Mouse Testis and Placenta tissue lysates.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab129726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa).|
相关性The Human testis expressed gene 19 (Tex19) is duplicated in mouse and rat to give two genes Tex19.1 and Tex19.2. Tex19.1 expression is limited to pluripotent cells and germ cells, and is downregulated after differentiation. The Tex19.1 (-/-) knockout male mice have impaired spermatogenesis.
- Testis expressed protein 19A antibody
- Tex 19.1 antibody
- Tex 19a antibody
- Tex19a antibody
All lanes : Anti-Tex19.1 antibody (ab129726) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate
Lane 2 : Mouse Placenta Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 45 kDa (possible non-specific binding)
Exposure time: 8 minutes
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab129726 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.