Application
Western blot
Sample
Human Cell lysate - whole cell (Hepatic Stellate Cells)
Gel Running Conditions
Reduced Denaturing (Gradient polyacrilamide gel 4-20%)
Loading amount
17 µg
Treatment
TGF 5ng/mL for 48 hrs
Specification
Hepatic Stellate Cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA (TBS, 0.1% Tween )
Dilution
1/500
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: IRDye® 800CW Goat anti-Mouse IgG Secondary Antibod
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Fluorescence 800nm
Negative control
Untreated human HSCs
Exposure
2 minute(s) and 0 second(s)
Positive control
Human HSCs treated with 5ng/mL TGF for 48 hours.
Additional data
Additional Notes
Cells lysed in RIPA buffer.
Protein transferred to PVDF membrane
Abcam response
Our Scientific Support team has investigated this negative Abreview and we have resolved the issue in accordance with our Abpromise..
It seems this antibody does not work for this application under these conditions.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 19 2021