Anti-Tenascin C抗体[T2H5] (ab3970)
Key features and details
- Mouse monoclonal [T2H5] to Tenascin C
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Tenascin C抗体[T2H5]
参阅全部 Tenascin C 一抗 -
描述
小鼠单克隆抗体[T2H5] to Tenascin C -
宿主
Mouse -
Tested Applications & Species
Application Species WB Human -
免疫原
Full length native protein (purified from a homogenate of a mammary tumour specimen) (Human).
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阳性对照
- IHC-P: Human tonsil tissue. WB: U-2 OS cell lysate.
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常规说明
Tenascin is a high molecular weight, multifunctional, hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM). The tenascin molecule is a disulfide-linked hexamer. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 8.0
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE. -
Primary antibody说明
Tenascin is a high molecular weight, multifunctional, hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM). The tenascin molecule is a disulfide-linked hexamer. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted. -
克隆
单克隆 -
克隆编号
T2H5 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
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WB |
Human
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应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 240 kDa.
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说明 |
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 240 kDa. |
靶标
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功能
Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6. -
序列相似性
Belongs to the tenascin family.
Contains 15 EGF-like domains.
Contains 1 fibrinogen C-terminal domain.
Contains 15 fibronectin type-III domains. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 3371 Human
- Omim: 187380 Human
- SwissProt: P24821 Human
- Unigene: 143250 Human
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别名
- 150 225 antibody
- Cytotactin antibody
- Glioma associated extracellular matrix antigen antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tenascin C antibody [T2H5] (ab3970)
ab3970 staining paraffin embedded tonsil tissue sections.
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Anti-Tenascin C antibody [T2H5] (ab3970) at 1 µg/ml + U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 265 kDa why is the actual band size different from the predicted?
Additional bands at: 34 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
Tenascin C contains an exstensive number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
实验方案
文献 (14)
ab3970 被引用在 14 文献中.
- Brechbuhl HM et al. Fibroblast subtypes define a metastatic matrisome in breast cancer. JCI Insight 5:N/A (2020). PubMed: 32045383
- Molina-Molina M et al. Anti-fibrotic effects of pirfenidone and rapamycin in primary IPF fibroblasts and human alveolar epithelial cells. BMC Pulm Med 18:63 (2018). PubMed: 29703175
- Valnegri P et al. RNF8/UBC13 ubiquitin signaling suppresses synapse formation in the mammalian brain. Nat Commun 8:1271 (2017). PubMed: 29097665
- Uchimura T et al. Insulin-Like Growth Factor II (IGF-II) Inhibits IL-1ß-Induced Cartilage Matrix Loss and Promotes Cartilage Integrity in Experimental Osteoarthritis. J Cell Biochem 116:2858-69 (2015). PubMed: 26015264
- Razafsky D & Hodzic D A variant of Nesprin1 giant devoid of KASH domain underlies the molecular etiology of autosomal recessive cerebellar ataxia type I. Neurobiol Dis 78:57-67 (2015). PubMed: 25843669