JavaScript is disabled in your browser. Please enable JavaScript to view this website.

Histone methyltransferase and demethylase inhibitors

Study the function of histone H3 methyltransferase and demethylase with these high quality and purified inhibitors.

Explore our best-selling histone H3 methyltransferase inhibitor

Study the function of histone H3 methyltransferase and demethylase with these high quality and purified inhibitors.

​​​Histone H3 methyltransferase inhibitors

Histone methyltransferase (HMT) inhibitors can be grouped by their specificity for different types of methyltransferases. Both lysine and arginine HMTs use S-adenosylmethionine (SAM) as a co-factor and methyl group donor. Lysine-specific  HMTs fall into two groups:

(1) Su(var)3-9, Enhancer of zeste, Trithorax (SET) domain-containing, which methylate lysines in the histone tails

(2) Non-SET domain containing, which methylate histone cores

Below, we list selected inhibitors that can be used to study the function of key SET domain-containing histone H3 HMTs:

Writer (mark added)
Inhibitor
Product code
Notes
EZH2 (H3K27me3)
3-deazaneplanocin A
3-deazaneplanocin A hydrochloride (DZNep)
ab142215
ab145628
Also inhibits trimethylation of H4K20 in vitro
EZH2 (H3K27me3)
UNC1999
ab146152
22-fold selectivity over EZH1
KMT1A/ SUV39 (H3K9me3)
Chaetocin
ab144534
Also inhibits other K9-specific HMTs
SET domain- containing HMTs
Sinefungin
ab144518
Non-selective inhibitor

Histone H3 demethylase inhibitors

Histone demethylase inhibitors can also be grouped by their specificity for different types of demethylases. Histone demethylases can be divided into 2 main groups:

(1) The flavin adenine dinucleotide (FAD)-dependent amine oxidases, such as the lysine-specific demethylase (LSD) family. These demethylate mono-and dimethylated substrates.

(2) The Fe(II) and α​-ketoglutarate-dependent dioxygenases (2-oxoglutarate dioxygenases), which include the Jumonji C (JmjC) domain-containing family. These demethylate mono-, di- and trimethylated substrates.

Key histone H3 demethylase inhibitors and their specificities are described below.

Eraser (mark removed)
Inhibitor
Product code
Notes
KDM6A/ UTX and KDM6B / JMJD3 (H3K27me3
GSK-J1
ab144396
Also inhibits other H3K27me3 demethylases and the H3K4me demethylases KDM5B/PLU1/JARID1B and KDM5C/Jarid1C/SMCX
GSK-J2
ab144398
GSK-J1 isomer for use as a less-active control
GSK-J4
ab144395
Pro-drug of GSK-J1 for cellular use
GSK-J5
ab144397
Pro-drug of GSK-J2 for cellular use
KDM2/7 JMJC subfamily of histone demethylases
Daminozide
ab144621
Highly selective over other subfamily members (KDM3, KDM4, KDM5 and KDM6) and other 2-oxoglutarate oxygenases
JMJC-domain containing histone demethylases
IOX1
ab144394
Inhibits most 2-oxoglutarate oxygenases
Methylstat
ab144566
JIB-04
ab144818