重组Anti-TDP43抗体[DB9] (ab254166)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [DB9] to TDP43
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-TDP43抗体[DB9]
参阅全部 TDP43 一抗 -
描述
小鼠单克隆抗体[DB9] to TDP43 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, ELISAmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Common marmoset -
免疫原
Recombinant fragment within Human TDP43 aa 200 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q13148 -
阳性对照
- WB: HeLa, Jurkat, K562, HEK-293T, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates. IHC-P: Human cerebrum and colon tissue; mouse cerebrum tissue; rat cerebrum tissue.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
DB9 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab254166于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.467 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
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IHC-P | (4) |
Use a concentration of 4.67 - 23.35 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Human: 4.67µg/ml Mouse and rat: 23.35µg/ml |
ELISA |
Use at an assay dependent concentration.
1000-10000 ng/mL |
说明 |
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WB
Use a concentration of 0.467 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). |
IHC-P
Use a concentration of 4.67 - 23.35 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Human: 4.67µg/ml Mouse and rat: 23.35µg/ml |
ELISA
Use at an assay dependent concentration. 1000-10000 ng/mL |
靶标
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功能
DNA and RNA-binding protein which regulates transcription and splicing. Involved in the regulation of CFTR splicing. It promotes CFTR exon 9 skipping by binding to the UG repeated motifs in the polymorphic region near the 3'-splice site of this exon. The resulting aberrant splicing is associated with pathological features typical of cystic fibrosis. May also be involved in microRNA biogenesis, apoptosis and cell division. Can repress HIV-1 transcription by binding to the HIV-1 long terminal repeat. Stabilizes the low molecular weight neurofilament (NFL) mRNA through a direct interaction with the 3' UTR. -
组织特异性
Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen. -
疾病相关
Defects in TARDBP are the cause of amyotrophic lateral sclerosis type 10 (ALS10) [MIM:612069]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology of ALS is likely to be multifactorial, involving both genetic and environmental factors. The disease is inherited in 5-10% of the cases. -
序列相似性
Contains 2 RRM (RNA recognition motif) domains. -
结构域
The RRM domains can bind to both DNA and RNA. -
翻译后修饰
Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU. -
细胞定位
Nucleus. In patients with frontotemporal lobar degeneration and amyotrophic lateral sclerosis, it is absent from the nucleus of affected neurons but it is the primary component of cytoplasmic ubiquitin-positive inclusion bodies. - Information by UniProt
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数据库链接
- Entrez Gene: 23435 Human
- Entrez Gene: 230908 Mouse
- Entrez Gene: 298648 Rat
- Omim: 605078 Human
- SwissProt: Q13148 Human
- SwissProt: Q921F2 Mouse
- Unigene: 300624 Human
- Unigene: 635053 Human
see all -
别名
- ALS10 antibody
- OTTHUMP00000002171 antibody
- OTTHUMP00000002172 antibody
see all
图片
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All lanes : Anti-TDP43 antibody [DB9] (ab254166) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg
Lane 3 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg
Lane 4 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
Lane 5 : C6 (rat glial tumor glial cell), whole cell lysate at 10 µg
Lane 6 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg
Lane 7 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 10 µg
Lane 8 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 10 µg
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/100000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDaBlocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lanes 1-4: 103 seconds;
Lanes 5-8: 48 seconds. -
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling TDP43 with ab254166 at 4.67µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on human colon is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling TDP43 with ab254166 at 23.35µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on rat cerebrum is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TDP43 with ab254166 at 23.25µg/ml, followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on mouse cerebrum is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling TDP43 with ab254166 at 4.67µg/ml followed by Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use. Nuclear staining on human cerebrum is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879) ready to use.Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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TDP43 = 5000 ng/mL.
ab254166 used at 0 - 10,000 ng/mL. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L)secondary antibody was used at 1000ng/ml
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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