Application
Western blot
Sample
Mouse Cell lysate - whole cell (myoblase and C2C12)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
3 µg
Specification
myoblase and C2C12
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% non fat milk
Secondary antibody
Name
Non-Abcam antibody was used: jackson labs hrp anti mouse secondary
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 45 weak kDa Non-specific: 38, 56 kDa
Additional data
Abcam response
This Abreview was produced as a part of our Abtrial program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. wei xia
Verified customer
提交于 Feb 25 2019