Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (4-20% gradient Tris-Gly gel)
Sample
Mouse Cell lysate - whole cell (MEFs)
Specification
MEFs
Blocking step
LI-COR Odyssey Blocking Buffer as blocking agent for 45 minute(s) · Concentration: 50% · Temperature: RT°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocker 1:1 in PBS + 0.1%Tween
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: AlexaFluor 680 goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Dilution
1/10000
Detection
Detection method
LI-COR Odyssey detection system
Bands
Specific: 68 kDa Non-specific: 90, 50, 48, 17 kDa
Exposure
2 minute(s) and 0 second(s)
Negative control
-
Positive control
MEF whole cell extract
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提交于 Sep 19 2014