Anti-TCR V beta 5抗体[MEM-262] (ab1898)

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Key features and details

  • Mouse monoclonal [MEM-262] to TCR V beta 5
  • Suitable for: Flow Cyt, IP, WB
  • Reacts with: Human
  • Isotype: IgG2a

概述

  • 产品名称

    Anti-TCR V beta 5抗体[MEM-262]

  • 描述

    小鼠单克隆抗体[MEM-262] to TCR V beta 5

  • 宿主

    Mouse

  • 特异性

    The antibody recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.

  • 经测试应用

    适用于: Flow Cyt, IP, WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Human thymoma cell line HPB-ALL.

  • 常规说明

    Functional Application Activates T cells (V beta 5-related subset).

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 纯化说明

    Purified from hybridoma culture supernatant. Purity >95% by SDS-PAGE.

  • 克隆

    单克隆

  • 克隆编号

    MEM-262

  • 同种型

    IgG2a

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab1898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Uniquely recognizes denatured TCR beta chains under the conditions of Western blotting.

靶标

  • 相关性

    T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. The Vß5 TCR is a variant of the TCR ß chain. It belongs to Ig superfamily and is expressed by a subset of peripheral blood T cells and some T cell clones. TCR Vß5 has been reported to recognize some staphylococcus bacterial superantigens.

  • 细胞定位

    Cell Membrane

  • 数据库链接

    • 别名

      • T cell receptor antibody
      • TCB antibody
      • TCRB antibody
      • TRB antibody
      • TRB@ antibody
      see all

    文献

    发表研究结果有使用 ab1898？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab1898 被引用在 3 文献中.

    • Wang CT  et al. Attenuating systemic inflammatory markers in simulated high-altitude exposure by heat shock protein 70-mediated hypobaric hypoxia preconditioning in rats. J Formos Med Assoc 114:328-38 (2015). PubMed: 25839766
    • Grabherr MG  et al. Exploiting nucleotide composition to engineer promoters. PLoS One 6:e20136 (2011). PubMed: 21625601
    • Pavlistová D  et al. A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor. Immunol Lett 88:105-8 (2003). PubMed: 12880678

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    Question

    Which lysates are recommended as positive controls for these antibodies?

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    Answer

    Thank you for your patience. For ab9219, the originator has recommended the following. Since the antibody recognizes human cytokeratin 18 we recommend using the PBS-insoluble fraction (pellet) of human cell lines containing cytokeratin 18 (for example Hela or T24, which was used for immunization of the mice) as a positive control in WB. You may also want to refer to the following publications for additional information about this antibody: Ramaekers F et al. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol 136:641-55 (1990). PubMed: 1690512 Schaafsma HE et al. Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression. Am J Pathol 136:329-43 (1990). PubMed: 1689541 If you have any additional questions, please contact us again.

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    Question

    Which lysates are recommended as positive controls for these antibodies?

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    Answer

    Thank you for your enquiry. The originator of ab1898 was able to provide me with the following information. Western blot detection: -material: HPB-ALL cell line -non-reducing conditions -detects only free beta chains -molecular weight 36 kDa The following publication has used this antibody and you may find some additional here as well: Pavlistová D et al. A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor. Immunol Lett 88:105-8 (2003). PubMed: 12880678 I have not yet received information regarding ab9219 and will contact you again when I do.

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