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Signal Transduction Protein Phosphorylation Tyrosine Phosphatases

Anti-TCPTP抗体(ab1996)

  • Datasheet
  • SDS
Submit a review Q&A (1)

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Western blot - Anti-TCPTP antibody (ab1996)

    Key features and details

    • Rabbit polyclonal to TCPTP
    • Suitable for: WB
    • Reacts with: Recombinant fragment
    • Isotype: IgG

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    Recombinant human TCPTP protein (ab42575)

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    概述

    • 产品名称

      Anti-TCPTP抗体
      参阅全部 TCPTP 一抗
    • 描述

      兔多克隆抗体to TCPTP
    • 宿主

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Recombinant fragment
      See all applications and species data
    • 免疫原

      Two separate synthetic peptides conjugated to KLH derived from within residues 300 - 400 of Human TCPTP.

      参阅Abcam的专有抗源政策 (Peptides available as ab14809 and ab14810.)
    • 阳性对照

      • This antibody recognises the recombinant TCPTP fusion protein (60kDa)in Western blots.
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Signal Transduction
      • Protein Phosphorylation
      • Tyrosine Phosphatases

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human TCPTP protein (ab42575)

    应用

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab1996 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    应用 Species
    WB
    Recombinant fragment
    All applications
    Human
    应用 Ab评论 说明
    WB
    1/1000. Predicted molecular weight: 48 kDa.
    说明
    WB
    1/1000. Predicted molecular weight: 48 kDa.

    靶标

    • 组织特异性

      Isoform PTPA is probably the major isoform. Isoform PTPB is expressed in T-cells and in placenta.
    • 序列相似性

      Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
      Contains 1 tyrosine-protein phosphatase domain.
    • 细胞定位

      Cytoplasm; Nucleus and Endoplasmic reticulum.
    • Target information above from: UniProt accession P17706 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 5771 Human
      • Omim: 176887 Human
      • SwissProt: P17706 Human
      • Unigene: 654527 Human
      • Unigene: 663373 Human
      • 别名

        • Protein tyrosine phosphatase non receptor type 2 antibody
        • PTN2 antibody
        • PTN2_HUMAN antibody
        • PTPN 2 antibody
        • Ptpn2 antibody
        • PTPT antibody
        • T cell protein tyrosine phosphatase antibody
        • T-cell protein-tyrosine phosphatase antibody
        • TC PTP antibody
        • TCELLPTP antibody
        • TCPTP antibody
        • Tyrosine protein phosphatase non receptor type 2 antibody
        • Tyrosine-protein phosphatase non-receptor type 2 antibody
        see all

      图片

      • Western blot - Anti-TCPTP antibody (ab1996)
        Western blot - Anti-TCPTP antibody (ab1996)Data provided by Purely Proteins
        All lanes : Anti-TCPTP antibody (ab1996) at 1/2000 dilution

        Lane 1 : PTP1B (PTPN1)
        Lane 2 : TCPTP (PTPN2)
        Lane 3 : PTPH1 (PTPN3)
        Lane 4 : MEG1 (PTPN4)
        Lane 5 : STEP (PTPN5)
        Lane 6 : SHP1 (PTPN6)
        Lane 7 : HePTP (PTPN7)
        Lane 8 : SHP2 (PTPN11)
        Lane 9 : BDP1 (PTPN18)

        Predicted band size: 48 kDa



        Recombinant PTP phosphatase domains were probed with ab1996 at 1/2000.  The antibody recognises the recombinant TCPTP fusion protein at ~60 kDa.

        Lane 1: PTP1B (PTPN1)
        Lane 2:TCPTP (PTPN2)
        Lane 3:PTPH1 (PTPN3)
        Lane 4:MEG1 (PTPN4)
        Lane 5:STEP (PTPN5)
        Lane 6:SHP1 (PTPN6)
        Lane 7:HePTP (PTPN7)
        Lane 8:SHP2 (PTPN11)
        Lane 9:BDP1 (PTPN18)

        Recombinant PTP phosphatase domains were probed with ab1996 at 1/2000. The antibody recognises the recombinant TCPTP fusion protein at ~60 kDa.

      实验方案

      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (0)

      发表研究结果有使用 ab1996?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab1996 尚未被引用在任何文献中。

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      Question

      BATCH NUMBER Lot 6575 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM wrong band size on normal western and doesn't appear to work in IP (heavy chain seen only) ABcam protocol followed SAMPLE human endothelial cells PRIMARY ANTIBODY 1 in 500 TCPTP Abcam SECONDARY ANTIBODY DAKO 2'Ab rabbit DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED background secondary antibody on blot IP without TCPTP antibody ANTIBODY STORAGE CONDITIONS at -20'C aliquoted into 5ul vols SAMPLE PREPARATION standard NP-40 and 2X sample loading buffer AMOUNT OF PROTEIN LOADED 25-30ug ELECTROPHORESIS/GEL CONDITIONS reducing gel 8-10% TRANSFER AND BLOCKING CONDITIONS standard BSA blocking wet blot HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes

      Read More

      Abcam community

      Verified customer

      Asked on Apr 05 2005

      Answer

      Thank you for your enquiry and for filling the on-line Questionnaire. This antibody has been tested for Western blot application only, and we do not have any information if it is suitable for IP. Unfortunately, we do not have any information on IP. Ab1996 has been characterised for Western blot assay using recombinant TCPTP fusion protein. As the datasheet indicates it is expected to cross-react with Human (100% identity with immunogen) due to sequence homology. We would suggest running purified protein and applying positive control (i.e. human T cell or placenta) along with the samples. Do the endothelial cells express TCPTB at high or low levels? Do you have any preliminary background information? When you prepare the cell lysate, use RIPA buffer rather than standard NP-40 and 2X sample loading buffer which contain a cocktail of different proteinase inhibitors. It is important to use RIPA in order to prevent the degradation of the target protein in the samples. Preparation of RIPA cell lysates for Western Blots: 1. Wash cell pellets once with ice-cold PBS. 2. Add 1 ml of RIPA buffer to 10x8 cells, incubate on ice for 20 min, vortex 2 to 3 times. 3. Centrifuge for 5 min at 4oC at maximum speed in a microfuge tube. 4. Transfer supernatant into clean tube. Measure protein concentration with a protein assay. 5. Adjust concentration to 5 mg/ml with RIPA lysis buffer. 6. Add equal volume of 2 x SDS sample buffer into cell lysate, boil for 5 min. 7. Store at -20oC for daily use or -80oC for long term. Avoid repeated freeze thaw cycles. RIPA Base Ingredients Tris-HCl: 50 mM, pH 7.4 NP-40: 1% Na-deoxycholate: 0.25% NaCl: 150 mM EDTA: 1 mM PMSF: 1 mM Aprotinin, leupeptin, pepstatin: 1 microgram/ml each RIPA Protease Inhibitors Phenylmethylsulfonyl fluoride (PMSF) (200 mM stock solution in isopropanol; store at room temperature) EDTA (calcium chelator; 100 mM stock solution in H2O, pH 7.4) Leupeptin (store frozen in aliquots, 1 mg/ml in H2O) Aprotinin (store frozen in aliquots, 1 mg/ml in H2O) Pepstatin (store frozen in aliquots, 1 mg/ml in methanol) We hope this information will be useful for you.

      Read More

      Abcam Scientific Support

      回复于 Apr 08 2005

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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