重组Anti-TCEA1抗体[EPR15036] (ab184158)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15036] to TCEA1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TCEA1抗体[EPR15036]
参阅全部 TCEA1 一抗 -
描述
兔单克隆抗体[EPR15036] to TCEA1 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Jurkat, HEK293, K562 cell lysates; HUVEC cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR15036 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab184158于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/10000 - 1/50000. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).
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ICC/IF |
1/250.
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IP |
1/60 - 1/80.
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说明 |
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WB
1/10000 - 1/50000. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa). |
ICC/IF
1/250. |
IP
1/60 - 1/80. |
靶标
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功能
Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. -
疾病相关
Note=A chromosomal aberration involving TCEA1 may be a cause of salivary gland pleiomorphic adenomas (PA) [181030]. Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1. -
序列相似性
Belongs to the TFS-II family.
Contains 1 TFIIS central domain.
Contains 1 TFIIS N-terminal domain.
Contains 1 TFIIS-type zinc finger. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 6917 Human
- Entrez Gene: 21399 Mouse
- Entrez Gene: 362479 Rat
- Entrez Gene: 498453 Rat
- Omim: 601425 Human
- SwissProt: P23193 Human
- SwissProt: P10711 Mouse
- SwissProt: Q4KLL0 Rat
see all -
别名
- GTF2S antibody
- SII antibody
- TCEA antibody
see all
图片
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All lanes : Anti-TCEA1 antibody [EPR15036] (ab184158) at 1/50000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : 293 cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted? -
Immunofluorescence analysis of paraformaldehyde-fixed HUVEC cells, staining TCEA1 (red) with ab184158 at 1/250 dilution. Alexa Fluor®555-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
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Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate, labeling TCEA1 immunoprecipitated using ab184158 at 1/50 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
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