重组Anti-TBR2 / Eomes抗体[EPR21950-241] (ab216870)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21950-241] to TBR2 / Eomes
- Suitable for: IHC-P, IHC-Fr, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-TBR2 / Eomes抗体[EPR21950-241]
参阅全部 TBR2 / Eomes 一抗 -
描述
兔单克隆抗体[EPR21950-241] to TBR2 / Eomes -
宿主
Rabbit -
经测试应用
适用于: IHC-P, IHC-Fr, ICC/IF, IPmore details
不适用于: Flow Cyt or WB -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Hamster, Cat, Dog, Pig, Non human primates, Common marmoset -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human tonsil, E14.5 mouse cerebral cortex and E14.5 rat cerebral cortex tissues. IHC-Fr: Mouse E14.5 cerebrum and Rat E14.5 cerebrum tissue. ICC/IF: Mouse primary neuron/glia cells. IP: Mouse E14 brain tissue lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR21950-241 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab216870于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (5) |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IHC-Fr | (1) |
1/100.
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ICC/IF | (1) |
1/100.
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IP |
1/50.
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说明 |
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IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
1/100. |
ICC/IF
1/100. |
IP
1/50. |
靶标
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功能
Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. -
组织特异性
Expressed in CD8+ T-cells. -
疾病相关
Note=A translocation t(3;10)(p24;q23) located 215 kb 3' to the EOMES gene but leading to loss of its expression was identified in a large consanguineous family. Homozygous silencing produces microcephaly associated with corpus callosum agenesis, bilateral polymicrogyria, ventricular dilatation and a small cerebellum. -
序列相似性
Contains 1 T-box DNA-binding domain. -
发展阶段
Detected at 7 weeks of development in the forebrain floorplate of the CNS. Expressed within the mantle layer and migrating neuroblasts of the telencephalon at 12.5 weeks of development. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 8320 Human
- Entrez Gene: 13813 Mouse
- Entrez Gene: 233242 Rat
- Entrez Gene: 316052 Rat
- Omim: 604615 Human
- SwissProt: O95936 Human
- SwissProt: O54839 Mouse
- Unigene: 591663 Human
see all -
别名
- eomes antibody
- EOMES_HUMAN antibody
- Eomesodermin antibody
see all
图片
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TBR2 / Eomes was immunoprecipitated from 0.35mg Mouse E14 brain tissue lysate 10μg with ab216870 at 1/50 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab216870 1/1000 dilution (0.67 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.
Lane 1: Mouse E14 brain tissue lysate 10 μg
Lane 2: ab216870 IP in Mouse E14 brain tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216870 in Mouse E14 brain tissue lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 min
The band is consistent with what has been described in the literature (PMID: 26749212).
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Immunocytochemistry confocal image showing nuclear staining in mouse primary neuron cells. Anti-TBR2 is stained with ab216870 in a 1/100 dilution, and 2µg/ml AlexaFluor®488 Goat anti-Rabbit secondary (ab150077). Nuclear counterstaining is DAPI (blue).
The negative control is ab11267 Anti-MAP2 mouse monoclonal antibody with ab150120 AlexaFluor®594 Goat anti-Mouse secondary.
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Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat E14.5 cerebrum tissue labeling EOMES with ab216870 at 1/100 (5.75 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Positive staining in rat embryonic cerebrum (PMID: 24223221) is observed.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
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Immunohistochemical analysis of paraffin-embedded E14.5 rat cerebral cortex tissue labeling EOMES with ab216870 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on E14.5 rat cerebral cortex (PMID: 18725516) is observed. The section was incubated with ab216870 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized frozen Mouse E14.5 cerebrum tissue stained for EOMES using ab216870 at 1/100 dilution in immunohistochemical analysis. The secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. The nuclear counterstain was DAPI (Blue). Positive staining in mouse embryonic cerebrum (PMID: 24223221) is observed. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
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Immunohistochemical analysis of paraffin-embedded E14.5 mouse cerebral cortex tissue labeling EOMES with ab216870 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on E14.5 mouse cerebral cortex (PMID: 18725516) The section was incubated with ab216870 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling EOMES with ab216870 at 1/1000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human tonsil is observed. The section was incubated with ab216870 for 30 mins at RT. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (5)
ab216870 被引用在 5 文献中.
- Zhou Y et al. Molecular landscapes of human hippocampal immature neurons across lifespan. Nature N/A:N/A (2022). IHC-Fr ; Human . PubMed: 35794479
- Wedge ME et al. Virally programmed extracellular vesicles sensitize cancer cells to oncolytic virus and small molecule therapy. Nat Commun 13:1898 (2022). PubMed: 35393414
- Maiani E et al. AMBRA1 regulates cyclin D to guard S-phase entry and genomic integrity. Nature 592:799-803 (2021). PubMed: 33854232
- Moreau MX et al. Single-cell transcriptomics of the early developing mouse cerebral cortex disentangle the spatial and temporal components of neuronal fate acquisition. Development 148:N/A (2021). PubMed: 34170322
- Yuan X et al. Identification and verification of EOMEs regulated network in Alopecia areata. Int Immunopharmacol 84:106544 (2020). IHC ; Human . PubMed: 32353685