概述

  • 产品名称
    Anti-TB Ag85A抗体
    参阅全部 TB Ag85A 一抗
  • 描述
    兔多克隆抗体to TB Ag85A
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Mouse, Other species
  • 免疫原

    Recombinant fragment corresponding to TB Ag85A aa 53-331. (Mycobacterium tuberculosis).
    Sequence:

    YLQVPSPSMGRDIKVQFQSGGANSPALYLLDGLRAQDDFSGWDINTPAFE WYDQSGLSVVMPVGGQSSFYSDWYQPACGKAGCQTYKWETFLTSELPGWL QANRHVKPTGSAVVGLSMAASSALTLAIYHPQQFVYAGAMSGLLDPSQAM GPTLIGLAMGDAGGYKASDMWGPKEDPAWQRNDPLLNVGKLIANNTRVWV YCGNGKPSDLGGNNLPAKFLEGFVRTSNIKFQDAYNAGGGHNGVFDFPDS GTHSWEYWGAQLNAMKPDLQRALGATPNT


    Database link: P9WQP2

  • 阳性对照
    • Recombinant Mycobacterium tuberculosis TB Ag85A protein.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液
    pH: 7.4
    Preservative: 0.03% Proclin
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    >95%,Protein G purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab193499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
ELISA Use at an assay dependent concentration.

靶标

  • 相关性
    Mycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31 kDa, Ag85A, B and C. These proteins are responsible for the high affinity of mycobacteria to fibronectin. All three proteins possesses a mycolyltransferase activity - which is required for the biogenesis of trehalose dimycolate (cord factor), a dominant structure necessary for maintaining cell wall integrity. Thus all three proteins are involved in the final stages of mycobacterial cell wall assembly.
  • 细胞定位
    Secreted; cell wall. Cytoplasm
  • 别名
    • 85 A antibody
    • 85A antibody
    • Ag85A antibody
    • Antigen 85 A antibody
    • Antigen 85 complex A antibody
    • Antigen 85A antibody
    • Fbp A antibody
    • FbpA antibody
    • Fibronectin binding protein A antibody
    • Mpt 44 antibody
    • Mpt44 antibody
    • Mycobacterium tuberculosis antigen 85A antibody
    • Mycolyl transferase 85 A antibody
    • Mycolyl transferase 85A antibody
    • Rv3804c antibody
    • Secreted antigen Ag85A antibody
    see all

图片

  • All lanes : Anti-TB Ag85A antibody (ab193499) at 2 µg/ml

    Lane 1 : Recombinant Mycobacterium tuberculosis TB Ag85A protein at 1 µg
    Lane 2 : Recombinant Mycobacterium tuberculosis TB Ag85A protein at 10 µg

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution

    Predicted band size: 36 kDa
    Observed band size: 36 kDa

实验方案

文献

This product has been referenced in:
  • Sun L  et al. Novel adjuvant for immunization against tuberculosis: DNA vaccine expressing Mycobacterium tuberculosis antigen 85A and interleukin-15 fusion product elicits strong immune responses in mice. Biotechnol Lett 39:1159-1166 (2017). WB ; Mouse . Read more (PubMed: 28470626) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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