Application
Western blot
Sample
Human Cell lysate - whole cell (Platelets)
Loading amount
20 µg
Specification
Platelets
Treatment
ADP for 30 min
Gel Running Conditions
Reduced Denaturing (4-20% Criterion 26 Well)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in PBST
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-mouse HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL+
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 37 kDa Non-specific: 95 kDa
Additional data
Additional Notes
The observed band is faint due to the fact that it is in the platelets. Although platelets are enucleate many nuclear proteins like NFkb and other proteins are present in them.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Mahesh Shivananjappa
Verified customer
提交于 Nov 30 2011