Anti-TATA binding蛋白TBP抗体[1TBP18] - ChIP Grade (ab818)
![ChIP - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)](https://www.abcam.cn/ps/products/0/ab818/Images/ab818-263656-anti-tata-binding-protein-tbp-antibody-1tbp18-chip-grade-chip.gif "ChIP - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)")
Key features and details
- Mouse monoclonal [1TBP18] to TATA binding protein TBP - ChIP Grade
- Suitable for: Flow Cyt, ChIP
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-TATA binding蛋白TBP抗体[1TBP18] - ChIP Grade
参阅全部 TATA binding protein TBP 一抗 -
描述
小鼠单克隆抗体[1TBP18] to TATA binding蛋白TBP - ChIP Grade -
宿主
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ChIP HumanFlow Cyt Human
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免疫原
Synthetic peptide (Human).
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表位
Within amino acid residues 1-20 of human, mouse and rat TBP. The specific epitope for this antibody is accessible when the C-terminal domain of TBP is removed or when TBP is complexed with DNA. -
常规说明
ab81216 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
This product was changed from ascites to tissue culture supernatant on 19/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
Purified from TCS -
克隆
单克隆 -
克隆编号
1TBP18 -
同种型
IgG1 -
研究领域
- Epigenetics and Nuclear Signaling
- Transcription
- Polymerase associated factors
- Pol II Transcription
- TFIIB
- Epigenetics and Nuclear Signaling
- Transcription
- Polymerase associated factors
- Pol II Transcription
- TFIID
相关产品
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ChIP Related Products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| 应用 | Species |
|---|---|
| ChIP |
Human
|
| Flow Cyt |
Human
|
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. We recommend Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879) secondary antibody |
|
| ChIP | (2) |
Use 5-10 µg for 25 µg of chromatin.
|
| 说明 |
|---|
|
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. We recommend Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879) secondary antibody |
|
ChIP
Use 5-10 µg for 25 µg of chromatin. |
靶标
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功能
General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. -
组织特异性
Widely expressed, with levels highest in the testis and ovary. -
疾病相关
Defects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease. -
序列相似性
Belongs to the TBP family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 6908 Human
- Omim: 600075 Human
- SwissProt: P20226 Human
- Unigene: 1100 Human
- Unigene: 590872 Human
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别名
- GTF2D antibody
- GTF2D1 antibody
- HDL4 antibody
see all
图片
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ChIP - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)
Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 8 µg of ab818 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The last wash was performed with final wash buffer containing 250 mM NaCl. The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located in the core promoter region of the genes.
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)](https://www.abcam.cn/ps/products/0/ab818/Images/ab818-3757-anti-tata-binding-protein-tbp-antibody-1tbp18-chip-grade-flow-cytometry.jpg)
Flow Cytometry - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)Overlay histogram showing HeLa cells stained with ab818 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab818, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879) secondary antibody at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (220)
ab818 被引用在 220 文献中.
- Chaudhari S et al. Inhibition of interleukin-6 on matrix protein production by glomerular mesangial cells and the pathway involved. Am J Physiol Renal Physiol 318:F1478-F1488 (2020). PubMed: 32390515
- Nakata T et al. Growth hormone activates X-box binding protein 1 in a sexually dimorphic manner through the extracellular signal-regulated protein kinase and CCAAT/enhancer-binding protein ß pathway in rat liver. Endocr J 67:185-200 (2020). PubMed: 31748431
- López de la Oliva AR et al. Nuclear Translocation of Glutaminase GLS2 in Human Cancer Cells Associates with Proliferation Arrest and Differentiation. Sci Rep 10:2259 (2020). PubMed: 32042057
- Han RW et al. Role of lateral amygdala calstabin2 in regulation of fear memory. Mol Brain 13:35 (2020). PubMed: 32151269
- Kim NY et al. Cinnamaldehyde protects against oxidative stress and inhibits the TNF-a-induced inflammatory response in human umbilical vein endothelial cells. Int J Mol Med 46:449-457 (2020). PubMed: 32319555