Anti-TATA binding蛋白TBP抗体[1TBP18] - ChIP Grade (ab818)

Reviews (39)Q&A (32)References (242)
ChIP - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)
  • Flow Cytometry - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)

Key features and details

  • Mouse monoclonal [1TBP18] to TATA binding protein TBP - ChIP Grade
  • Suitable for: ICC, IHC-Fr, IP, ICC/IF, IHC-P, ChIP/Chip, EMSA, ELISA, Flow Cyt, WB, ChIP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-TATA binding蛋白TBP抗体[1TBP18] - ChIP Grade
    参阅全部 TATA binding protein TBP 一抗

  • 描述

    小鼠单克隆抗体[1TBP18] to TATA binding蛋白TBP - ChIP Grade

  • 宿主

    Mouse

  • 经测试应用

    适用于: ICC, IHC-Fr, IP, ICC/IF, IHC-P, ChIP/Chip, EMSA, ELISA, Flow Cyt, WB, ChIPmore details

  • 种属反应性

    与反应: Mouse, Rat, Human
    不与反应: Saccharomyces cerevisiae, Xenopus laevis, Drosophila melanogaster, Silk worm

  • 免疫原

    Synthetic peptide corresponding to Human TATA binding protein TBP.

  • 表位

    Within amino acid residues 1-20 of human, mouse and rat TBP. The specific epitope for this antibody is accessible when the C-terminal domain of TBP is removed or when TBP is complexed with DNA.

  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 19/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab818于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC (1)
Use at an assay dependent concentration.
IHC-Fr (1)
Use at an assay dependent concentration.
IP (2)
Use at an assay dependent concentration.
ICC/IF (4)
Use at an assay dependent concentration.
IHC-P (1)
Use at an assay dependent concentration.
ChIP/Chip (1)
Use at an assay dependent concentration.
EMSA
Use at an assay dependent concentration.
ELISA (2)
Use at an assay dependent concentration.

We recommend Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab6729) secondary antibody.
Flow Cyt
Use 1µg for 106 cells.

ab170190 and ab81216 - Mouse monoclonal IgG1, are suitable for use as an isotype control with this antibody.

We recommend Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879) secondary antibody
WB (24)
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa. PubMed: 23767827
ChIP (2)
Use 5-10 µg for 25 µg of chromatin.
说明
ICC
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
ChIP/Chip
Use at an assay dependent concentration.
EMSA
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

We recommend Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab6729) secondary antibody.
Flow Cyt
Use 1µg for 106 cells.

ab170190 and ab81216 - Mouse monoclonal IgG1, are suitable for use as an isotype control with this antibody.

We recommend Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879) secondary antibody
WB
Use at an assay dependent concentration. Predicted molecular weight: 38 kDa. PubMed: 23767827
ChIP
Use 5-10 µg for 25 µg of chromatin.

靶标

  • 功能

    General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.

  • 组织特异性

    Widely expressed, with levels highest in the testis and ovary.

  • 疾病相关

    Defects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.

  • 序列相似性

    Belongs to the TBP family.

  • 细胞定位

    Nucleus.
  • Information by UniProt

  • 数据库链接

    • 别名

      • GTF2D antibody
      • GTF2D1 antibody
      • HDL4 antibody
      • MGC117320 antibody
      • MGC126054 antibody
      • MGC126055 antibody
      • SCA17 antibody
      • TATA binding factor antibody
      • TATA box factor antibody
      • TATA sequence binding protein antibody
      • TATA sequence-binding protein antibody
      • TATA-binding factor antibody
      • TATA-box binding protein N-terminal domain antibody
      • TATA-box factor antibody
      • TATA-box-binding protein antibody
      • TBP antibody
      • TBP_HUMAN antibody
      • TFIID antibody
      • Transcription initiation factor TFIID TBP subunit antibody
      see all

    图片

    • ChIP - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)
      ChIP - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)

      Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 8 µg of  ab818 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The last wash was performed with final wash buffer containing 250 mM NaCl. The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located in the core promoter region of the genes.

      This image was generated using the ascites version of the product. 

    • Flow Cytometry - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)
      Flow Cytometry - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)

      Overlay histogram showing HeLa cells stained with ab818 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab818, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879) secondary antibody at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

      This image was generated using the ascites version of the product.

    文献 (242)

    发表研究结果有使用 ab818？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab818 被引用在 242 文献中.

    • Alula KM  et al. Nuclear partitioning of Prohibitin 1 inhibits Wnt/ß-catenin-dependent intestinal tumorigenesis. Oncogene 40:369-383 (2021). PubMed: 33144683
    • Miao J  et al. Klotho retards renal fibrosis through targeting mitochondrial dysfunction and cellular senescence in renal tubular cells. Physiol Rep 9:e14696 (2021). PubMed: 33463897
    • Lohakul J  et al. The Protective Effect of Polyherbal Formulation, Harak Formula, on UVA-Induced Photoaging of Human Dermal Fibroblasts and Mouse Skin via Promoting Nrf2-Regulated Antioxidant Defense. Front Pharmacol 12:649820 (2021). PubMed: 33912060
    • Xu X  et al. The SWI/SNF-Related, Matrix Associated, Actin-Dependent Regulator of Chromatin A4 Core Complex Represses Respiratory Syncytial Virus-Induced Syncytia Formation and Subepithelial Myofibroblast Transition. Front Immunol 12:633654 (2021). PubMed: 33732255
    • Liu Y  et al. Hyperlipidemic hypersensitivity to lethal microbial inflammation and its reversal by selective targeting of nuclear transport shuttles. Sci Rep 11:11907 (2021). PubMed: 34099795
    View all Publications for this product

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    1-10 of 71 Abreviews or Q&A

    Western blot abreview for Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - nuclear (cell line)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    60 µg
    Specification
    cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 05 2021

    Western blot abreview for Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (cell line)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    50 µg
    Specification
    cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 05 2021

    Western blot abreview for Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Cell line)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    80 µg
    Specification
    Cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 01 2021

    Western blot abreview for Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - nuclear (Cell line)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    57 µg
    Specification
    Cell line
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 01 2021

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade

     Below Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Spinal cord)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    Yes - 0.2% triton x100
    Specification
    Spinal cord
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 27 2015

    Western blot abreview for Anti-TATA binding protein TBP [1TBP18] antibody - ChIP Grade

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    60 µg
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Sample
    Rat Cell lysate - nuclear (airway epithelial cell)
    Specification
    airway epithelial cell
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Joseph Cichocki

    Verified customer

    提交于 Mar 07 2014

    Western blot abreview for Anti-TATA binding protein TBP antibody [1TBP18] - Nuclear Loading Control and ChIP Grade

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Denaturing (5%)
    Sample
    Mouse Cell lysate - nuclear (liver)
    Specification
    liver
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    MS. Arlene Lim

    Verified customer

    提交于 Sep 04 2013

    Western blot abreview for Anti-TATA binding protein TBP antibody [1TBP18] - Nuclear Loading Control and ChIP Grade

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Hela nuclear lysate)
    Loading amount
    20 µg
    Specification
    Hela nuclear lysate
    Gel Running Conditions
    Reduced Denaturing (12)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 15 2013

    Question

    I noticed for your antibody, Anti-TATA binding protein TBP antibody [1TBP18] - Nuclear Loading Control and ChIP Grade (ab818), one of the tested applications is EMSA. I can't find any protocol on your website for how to use this antibody in EMSA. Can you please give me a protocol for this? Thanks in advance for your help!

    Read More
    Answer

    You can see this antibody used in a gel shift assay in Thompson NE et al. 2004 Protein Expression and Purification 36: 186-197. However, this publication (which must be purchased to view the entire article) refers to using a protocol for the gel shift assay found in Wiley SR et al. 1991 Proc Natl Acad Sci USA 89: 5814-5818 (available free of charge) with the following modifications:

    A 25bp oligonucleotide containing a consensus TATA element was purchased from Promega. The reaction volumes were 20ul and contained 8ng of 5’-end-labeled 32P-labeled oligonucleotide and 10ng of human TATA Binding Protein. The reactions were incubated at 23oC for 15 minutes. For the MAb supershift assays, 40ng of MAb was added, and the reactions were allowed to incubate at 23oC for an additional 15 minutes. The reactions were subjected to electrophoresis in a nondenaturing gradient (4-15%) polyacrylamide gel on a PhastSystem for a total of 140-300Vh. The gel and buffer strips were first soaked in a solution of 1/2x TBE and 2mM MgCl2; the MgCl2 helped to stabilize the TBP/DNA complex in the absence of TFIIB.

    Read More

    Question

    I received a replacement vial but it is still not wokring as expected.

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    Answer

    Thank you for completing our survey.

    I am sorry to hear the replacement product also didn't work as expected. I would like to offer you a credit as this product is still not working as expected. Please could you confirm whether this is to your satisfaction.

    I look forward to your reply.

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