Anti-TATA binding蛋白TBP抗体[1TBP18] - ChIP Grade (ab818)
- 数据表
- 参考文献 (186)
- 实验方案
概述
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产品名称Anti-TATA binding蛋白TBP抗体[1TBP18] - ChIP Grade
参阅全部 TATA binding protein TBP 一抗 -
描述小鼠单克隆抗体[1TBP18] to TATA binding蛋白TBP - ChIP Grade
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宿主Mouse
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经测试应用适用于: ChIP/Chip, ELISA, IHC-P, EMSA, IP, WB, Flow Cyt, ChIP, ICC/IF, ICC, IHC-Frmore details
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种属反应性与反应: Mouse, Rat, Human
不与反应: Saccharomyces cerevisiae, Xenopus laevis, Drosophila melanogaster, Silk worm -
免疫原
Synthetic peptide (Human).
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表位Within amino acid residues 1-20 of human, mouse and rat TBP. The specific epitope for this antibody is accessible when the C-terminal domain of TBP is removed or when TBP is complexed with DNA.
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阳性对照
- HeLa nuclear extract
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常规说明
ab81216 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
This product was changed from ascites to tissue culture supernatant on 19/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions please do not hesitate to contact our scientific support team.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium azide
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Concentration information loading... -
纯度Protein G purified
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纯化说明Purified from TCS
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克隆单克隆
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克隆编号1TBP18
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同种型IgG1
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研究领域
- Epigenetics and Nuclear Signaling
- Transcription
- Polymerase associated factors
- Pol II Transcription
- TFIIB
- Epigenetics and Nuclear Signaling
- Transcription
- Polymerase associated factors
- Pol II Transcription
- TFIID
相关产品
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ChIP Related Products
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Isotype control
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Related Products
应用
Our Abpromise guarantee covers the use of ab818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ChIP/Chip | Use at an assay dependent concentration. | |
| ELISA | 1/1000. We recommend Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab6729) secondary antibody. |
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| IHC-P | Use at an assay dependent concentration. | |
| EMSA | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| WB | 1/2000. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). PubMed: 23767827 | |
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. We recommend Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879) secondary antibody |
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| ChIP | Use 5-10 µg for 25 µg of chromatin. | |
| ICC/IF | Use at an assay dependent concentration. | |
| ICC | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. |
靶标
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功能General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
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组织特异性Widely expressed, with levels highest in the testis and ovary.
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疾病相关Defects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.
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序列相似性Belongs to the TBP family.
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细胞定位Nucleus.
- Information by UniProt
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数据库链接
- Entrez Gene: 6908 Human
- Entrez Gene: 21374 Mouse
- Entrez Gene: 117526 Rat
- Omim: 600075 Human
- SwissProt: P20226 Human
- SwissProt: P29037 Mouse
- Unigene: 1100 Human
- Unigene: 590872 Human
see all -
别名
- GTF2D antibody
- GTF2D1 antibody
- HDL4 antibody
see all
图片
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![Western blot - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)](https://www.abcam.cn/ps/products/0/ab818/Images/TATA-binding-protein-TBP-Primary-antibodies-ab818-12.jpg)
Western blot - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)This image is a courtesy of Chien Hsin LeeAll lanes : Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818) at 1/2000 dilution
Lane 1 : Human Huh7 nuclear cell lysate
Lane 2 : Human Huh7 cytoplast cell lysate
Lane 3 : Human HepG2 nuclear cell lysate
Lane 4 : Human HepG2 cytoplast cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : HRP-conjugated goat polyclonal to mouse IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 30 secondsThis image was generated using the ascites version of the product.
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![ChIP - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)](https://www.abcam.cn/ps/products/0/ab818/Images/ab818-263656-ab8185.gif)
ChIP - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 8 µg of ab818 (blue), and 20 µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The last wash was performed with final wash buffer containing 250 mM NaCl. The immunoprecipitated DNA was quantified by real time PCR (Taqman and sybr green approach). Primers and probes are located in the core promoter region of the genes.
This image was generated using the ascites version of the product.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)](https://www.abcam.cn/ps/products/0/ab818/Images/TATA-binding-protein-TBP-Primary-antibodies-ab818-20.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)This image is courtesy of anonymous Abreview.ab818 staining TATA binding protein TBP in Human stomach tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The tissue underwent formaldehyde fixation before heat mediated antigen retrieval in 10mM Citrate buffer pH 6.0. Blocking was done in 5% serum for 1 hour at 23°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 23°C. An HRP-conjugated Goat polyclonal to Mouse IgG was used undiluted as the secondary antibody.
This image was generated using the ascites version of the product.
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![Flow Cytometry - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)](https://www.abcam.cn/ps/products/0/ab818/Images/TATA-binding-protein-TBP-Primary-antibodies-ab818-40.jpg)
Flow Cytometry - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)Overlay histogram showing HeLA cells stained with ab818 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab818, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879) secondary antibody at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)](https://www.abcam.cn/ps/products/0/ab818/Images/TATA-binding-protein-TBP-Primary-antibodies-ab818-33.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)Image courtesy of an anonymous Abreview.ab818 staining TATA binding protein TBP in NIH3T3 mouse fibroblast cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.025% Triton X and then blocked using 5% serum for 1 hour at 23°C. Samples were then incubated with primary antibody at 1/200 for 16 hours at 4°C. The secondary antibody used was a goat anti-mouse IgG conjugated to Alexa Fluor® 350 (blue) used at a 1/1000 dilution.
This image was generated using the ascites version of the product.
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![ELISA - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)](https://www.abcam.cn/ps/products/0/ab818/Images/TATA-binding-protein-TBP-Primary-antibodies-ab818-8.jpg)
ELISA - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)This image is a courtesy of Anonymous Abreviewab818 staining TATA binding protein TBP by ELISA. The sample was purified from human AGS gastric carcinoma cell line and blocked with 5% BSA for 1 hour at 25°C. The primary antibody was used at 1/1000 dilution, and incubated with sample for 16 hour at 4°C. Rabbit Anti-Mouse IgG H&L (Alkaline Phosphatase) (ab6729) secondary antibody was used as secondary, diluted at 1/1000.
This image was generated using the ascites version of the product.
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![Western blot - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)](https://www.abcam.cn/ps/products/0/ab818/Images/ab818_2.jpg)
Western blot - Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818)Anti-TATA binding protein TBP antibody [1TBP18] - ChIP Grade (ab818) at 1/2000 dilution + Ros C cells with endogenous TBP
Performed under reducing conditions.
Predicted band size: 38 kDaReview by Jilin Liu submitted 19 August 2004.
This image was generated using the ascites version of the product.
实验方案
文献
This product has been referenced in:
- Cai T et al. FHL2 promotes tubular epithelial-to-mesenchymal transition through modulating ß-catenin signalling. J Cell Mol Med 22:1684-1695 (2018). WB ; Rat . Read more (PubMed: 29193729) »
- Yin J et al. Dkk3 dependent transcriptional regulation controls age related skeletal muscle atrophy. Nat Commun 9:1752 (2018). Read more (PubMed: 29717119) »
