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NGF

GeneName

NGF

Summary

NGF, also known as nerve growth factor, beta NGF, or beta nerve growth factor, is a 27 kDa secreted protein that plays a crucial role in the growth, maintenance, and survival of neurons. It is primarily expressed in the extracellular space and is involved in various cellular processes such as axon extension and neuron differentiation. NGF binds to its high-affinity receptor, TrkA, activating signalling pathways that promote neuronal survival and development. It is also implicated in modulating synaptic transmission and has roles in apoptotic signalling pathways, influencing both neuron maturation and the regulation of neurotransmitter secretion.

Importance

NGF is relevant to: - Neurodevelopmental research due to its essential role in neuronal growth and survival - Pain mechanisms, as it is involved in the sensory perception of pain and can influence pain pathways - Neurodegenerative diseases, where its modulation of neuronal health may provide insights into therapeutic strategies - Regenerative medicine, particularly in strategies aimed at enhancing nerve repair and regeneration following injury

Top Products

For researchers investigating NGF, we highly recommend the top-selling recombinant antibody, Anti-NGF antibody [EP1320Y] (ab52918). This antibody has been validated for use in Western blotting (WB) and immunocytochemistry (ICC), making it a versatile tool for various experimental setups. With 121 citations, it is well-regarded in the research community, reflecting its reliability and effectiveness in NGF studies. This recombinant antibody ensures batch-to-batch consistency, providing confidence in your experimental results. The Human beta Nerve Growth Factor ELISA Kit (ab193760), supported by 7 citations, is an excellent option for researchers looking to accurately measure NGF levels in their samples.

Abcam Product Citation Summary

The data indicates that NGF is being extensively studied in various contexts, particularly in relation to pancreatic cancer and neuroinflammation. The use of Abcam antibodies in both Western blotting and immunohistochemistry highlights the importance of NGF in understanding tumour biology and neuroregenerative processes. The studies span across different species, including human and rat models, suggesting a broad interest in the role of NGF in both cancer and nerve regeneration.

Abcam Product Citation Table

Product Code
Species
Application
Study Context
PMID
ab52918
Mouse
WB
Hippocampus
25514676
ab52918
Human
WB, IHC
Panc-1 cells
28440296
ab52918
Human
WB, IHC
Subcutaneous pancreatic tumours
28440296
ab52918
Human
WB, IHC
Orthotopic pancreatic tumours
28440296
ab52918
Human
WB
Corneal epithelial cells
31889912
ab52918
Human
WB, IHC
Pancreatic PDX tumours
28440296
ab6199
Mouse
IF
Tumour and stroma tissue
27000374
ab6199
Rat
WB
Schwann cell regulation
32042324
ab6199
Rat
WB
CFA treatment
31980031
ab6199
Rat
WB
Sciatic nerve tissues
32544883
ab99986
Human
WB
NPM‐ALK+ T‐cell lymphoma
28557340
ab99986
Human
WB
Pro-inflammatory/pain-related neurogenic response
27853274
ab99986
Human
WB
NP cells at varying pH levels
27853274

Function

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems (PubMed:14976160, PubMed:20978020). Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable) (PubMed:20978020). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro) (By similarity). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form (By similarity).

Involvement in disease

Neuropathy, hereditary sensory and autonomic, 5

HSAN5

A form of hereditary sensory and autonomic neuropathy, a genetically and clinically heterogeneous group of disorders characterized by degeneration of dorsal root and autonomic ganglion cells, and by sensory and/or autonomic abnormalities. HSAN5 patients manifest loss of pain perception and impaired temperature sensitivity, ulcers, and in some cases self-mutilation. The autonomic involvement is variable.

None

The disease is caused by variants affecting the gene represented in this entry.

Sequence Similarities

Belongs to the NGF-beta family.

Cellular localization

Alternative names

NGFB, NGF, Beta-nerve growth factor, Beta-NGF

swissprot:P01138 omim:162030 entrezGene:4803