CXCL8

GeneName

CXCL8

Summary

CXCL8, also known as IL-8 or interleukin 8, is an 11 kDa chemokine that is secreted into the extracellular region and space. It plays a pivotal role in mediating inflammatory responses by attracting neutrophils to sites of infection or injury through chemotaxis. CXCL8 binds to CXCR chemokine receptors and has functions in various biological processes, including angiogenesis, neutrophil activation, and responses to pro-inflammatory cytokines such as interleukin-1 and tumor necrosis factor. Its expression is particularly notable in inflammatory tissues, where it contributes to the regulation of immune responses and cell adhesion.

Importance

CXCL8 is relevant to: - The recruitment and activation of neutrophils during inflammation, which is essential for host defence against pathogens - The regulation of angiogenesis, impacting wound healing and tissue repair - The modulation of immune responses in various diseases, including autoimmune disorders and cancer - The potential development of therapeutic targets for conditions characterised by excessive inflammation or impaired immune responses

Top Products

For researchers investigating CXCL8, we recommend two primary antibodies that cater to different experimental needs. The first is the well-cited polyclonal antibody, Anti-IL-8 antibody (ab7747), which has garnered 73 citations, highlighting its reliability in ELISA applications. This antibody is a trusted choice for those focusing on quantifying IL-8 levels. Additionally, we offer the recombinant antibody, Anti-IL-8 antibody [EPR22994-255] (ab235584), which has been validated in knockout models and is suitable for Western blotting (WB). With 5 citations, this product is gaining traction in the research community. The recombinant nature of this antibody ensures batch-to-batch consistency, making it an excellent option for researchers seeking dependable performance in their experiments. The Recombinant human IL-8 protein (Active) ELISA Kit (ab259397), supported by 4 citations, is an excellent option for researchers looking to accurately measure CXCL8 levels in their samples.

Abcam Product Citation Summary

The data indicates that CXCL8 is being extensively studied in various human contexts, particularly in relation to cancer, inflammation, and immune responses. The use of multiple antibodies highlights the importance of CXCL8 in different biological processes, including tumor cell interactions and responses to hypoxia. The studies span a range of applications, primarily Western blotting, suggesting a focus on protein expression and function in human samples.

Abcam Product Citation Table

Function

Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed:18692776, PubMed:7636208). Also plays an important role in neutrophil activation (PubMed:2145175, PubMed:9623510). Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells (PubMed:1840701, PubMed:1891716). G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways (PubMed:11971003, PubMed:8662698).

Post-translational modifications

Several N-terminal processed forms are produced by proteolytic cleavage after secretion from at least peripheral blood monocytes, leukcocytes and endothelial cells. In general, IL-8(1-77) is referred to as interleukin-8. IL-8(6-77) is the most promiment form.

Citrullination at Arg-27 prevents proteolysis, and dampens tissue inflammation, it also enhances leukocytosis, possibly through impaired chemokine clearance from the blood circulation.

(Microbial infection) Cleaved by group A Streptococcus protease SpyCEP; leading to impaired neutrophil endothelial transmigration and thus increased virulence.

Sequence Similarities

Belongs to the intercrine alpha (chemokine CxC) family.

Cellular localization

• Secreted

Alternative names

IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1

Database links

swissprot:P10145 entrezGene:3576 omim:146930

Other research areas

• Immuno-oncology
• Neuroscience