CDK6

GeneName

CDK6

Summary

CDK6, also known as cyclin dependent kinase 6, CRK2, or CDK-6, is a 37 kDa serine/threonine kinase that plays a pivotal role in cell cycle regulation. It is primarily expressed in the nucleus and cytoplasm, forming complexes with cyclins D1, D2, and D3, which are essential for the transition from the G1 phase to the S phase of the cell cycle. CDK6 is involved in various biological processes, including cell division, DNA damage response, and differentiation of hematopoietic stem cells. Additionally, it participates in the regulation of cell motility and adhesion, influencing processes such as gliogenesis and neuronal development.

Importance

CDK6 is relevant to: - Cancer research due to its role in cell cycle regulation and its overexpression in various malignancies - Developmental biology, particularly in neurogenesis and astrocyte development - Understanding cellular senescence and differentiation, which has implications for regenerative medicine - Immune system regulation through its involvement in T cell differentiation and hematopoietic stem cell differentiation

Top Products

For researchers investigating CDK6, we highly recommend the top-selling recombinant antibody, Anti-Cdk6 antibody [EPR4515] (ab124821). This antibody has been validated in knockout models, ensuring its reliability in various applications, including Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), and flow cytometry (FC). With 138 citations, it is well-regarded in the research community, making it an excellent choice for those seeking robust and consistent results in their studies of CDK6. The Anti-Cdk6 antibody ELISA Kit (ab241554), supported by 5 citations, is an excellent option for researchers looking to accurately measure CDK6 levels in their samples.

Abcam Product Citation Summary

The data indicates that CDK6 is frequently studied in the context of human cell lines, particularly in relation to cell differentiation, proliferation, and cancer. Various studies utilise western blotting to investigate the regulation of CDK6 and its role in different cellular processes, including monocyte/macrophage differentiation and the effects of specific compounds on cellular signalling pathways.

Abcam Product Citation Table

Function

Serine/threonine-protein kinase involved in the control of the cell cycle and differentiation; promotes G1/S transition. Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclins during interphase at G1 to form a pRB/RB1 kinase and controls the entrance into the cell cycle. Involved in initiation and maintenance of cell cycle exit during cell differentiation; prevents cell proliferation and negatively regulates cell differentiation, but is required for the proliferation of specific cell types (e.g. erythroid and hematopoietic cells). Essential for cell proliferation within the dentate gyrus of the hippocampus and the subventricular zone of the lateral ventricles. Required during thymocyte development. Promotes the production of newborn neurons, probably by modulating G1 length. Promotes, at least in astrocytes, changes in patterns of gene expression, changes in the actin cytoskeleton including loss of stress fibers, and enhanced motility during cell differentiation. Prevents myeloid differentiation by interfering with RUNX1 and reducing its transcription transactivation activity, but promotes proliferation of normal myeloid progenitors. Delays senescence. Promotes the proliferation of beta-cells in pancreatic islets of Langerhans. May play a role in the centrosome organization during the cell cycle phases (PubMed:23918663).

Involvement in disease

Microcephaly 12, primary, autosomal recessive

MCPH12

A form of microcephaly, a disease defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small.

None

The disease is caused by variants affecting the gene represented in this entry.

Post-translational modifications

Thr-177 phosphorylation and Tyr-24 dephosphorylation promotes kinase activity.

Sequence Similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Tissue Specificity

Expressed ubiquitously. Accumulates in squamous cell carcinomas, proliferating hematopoietic progenitor cells, beta-cells of pancreatic islets of Langerhans, and neuroblastomas. Reduced levels in differentiating cells.

Cellular localization

• Cytoplasm
• Nucleus
• Cell projection
• Ruffle
• Cytoplasm
• Cytoskeleton
• Microtubule organizing center
• Centrosome
• Localized to the ruffling edge of spreading fibroblasts. Kinase activity only in nucleus. Localized to the cytosol of neurons and showed prominent staining around either side of the nucleus (By similarity). Present in the cytosol and in the nucleus in interphase cells and at the centrosome during mitosis from prophase to telophase (PubMed:23918663).

Alternative names

CDKN6, CDK6, Cyclin-dependent kinase 6, Cell division protein kinase 6, Serine/threonine-protein kinase PLSTIRE

Database links

swissprot:Q00534 entrezGene:1021 entrezGene:595 entrezGene:896 swissprot:P30281 swissprot:P24385 omim:603368 omim:168461 omim:123834

Other research areas

• Epigenetics
• Immuno-oncology