CD40LG
GeneName
CD40LG
Summary
CD40LG, also known as CD40L, SNAP-25, or TRAP, is a 29kDa glycoprotein that is primarily expressed on the surface of activated T cells and plays a pivotal role in immune responses. It is localised to the cell surface and extracellular space, and is involved in the binding to the CD40 receptor on B cells and other immune cells, facilitating crucial processes such as B cell differentiation, proliferation, and isotype switching. CD40LG also participates in various signalling pathways, including the CD40 signalling pathway and integrin-mediated signalling, and is implicated in the regulation of cytokine production and inflammatory responses.
Importance
CD40LG is relevant to: - The activation and regulation of B cells, which are essential for antibody production and adaptive immunity - T cell costimulation, enhancing T cell responses during immune activation - The inflammatory response, contributing to the pathogenesis of autoimmune diseases and chronic inflammation - Platelet activation and interactions with leukocytes, influencing vascular biology and thrombosis
Top Products
For researchers investigating CD40LG, we recommend two excellent primary antibodies that cater to various experimental needs. The first is the well-cited polyclonal antibody, Anti-TRAP/CD40L antibody (ab65854), which has garnered 20 citations and is highly regarded for its performance in both Western blotting (WB) and immunohistochemistry (IHC). This antibody is a trusted choice for those requiring reliable detection of CD40LG in diverse applications.Additionally, we offer the recombinant antibody, Anti-TRAP/CD40L antibody [EP462E] (ab52750), which has also been validated for use in Western blotting (WB) and has 19 citations to its name. As a recombinant product, it provides the batch-to-batch consistency that many researchers seek. Together, these antibodies provide robust options for studying CD40LG in various experimental contexts. The Human CD40L ELISA Kit (TNFSF5) (ab196268), supported by 2 citations, is an excellent option for researchers looking to accurately measure CD40L levels in their samples.
Abcam Product Citation Summary
The data indicates that CD40LG is being investigated in the context of human nasal tissues, specifically related to Eosinophilic Chronic Rhinosinusitis (ECRS). The use of immunohistochemistry suggests a focus on the localisation and expression patterns of this target in the study.
Abcam Product Citation Table
Function
Cytokine that acts as a ligand to CD40/TNFRSF5 (PubMed:1280226, PubMed:31331973). Costimulates T-cell proliferation and cytokine production (PubMed:8617933). Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation (PubMed:8617933). Induces the activation of NF-kappa-B (PubMed:15067037, PubMed:31331973). Induces the activation of kinases MAPK8 and PAK2 in T-cells (PubMed:15067037). Induces tyrosine phosphorylation of isoform 3 of CD28 (PubMed:15067037). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4 (By similarity). Involved in immunoglobulin class switching (By similarity).
CD40 ligand, soluble form
Acts as a ligand for integrins, specifically ITGA5:ITGB1 and ITGAV:ITGB3; both integrins and the CD40 receptor are required for activation of CD40-CD40LG signaling, which have cell-type dependent effects, such as B-cell activation, NF-kappa-B signaling and anti-apoptotic signaling.
Involvement in disease
Immunodeficiency with hyper-IgM, type 1
HIGM1
Immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence.
None
The disease is caused by variants affecting the gene represented in this entry.
Post-translational modifications
The soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated.
Sequence Similarities
Belongs to the tumor necrosis factor family.
Tissue Specificity
Specifically expressed on activated CD4+ T-lymphocytes.
Cellular localization
- Cell membrane
- Single-pass type II membrane protein
- Cell surface
- CD40 ligand, soluble form
- Secreted
- Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and a matrix metalloproteinases (MMP) inhibitor-sensitive pathway.
Alternative names
CD154, CD40L, TNFSF5, TRAP, CD40LG, CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, Tumor necrosis factor ligand superfamily member 5
Database links
swissprot:P29965 entrezGene:959 omim:300386
Other research areas
- Immunology & Infectious Disease